Advanced Protocols for Animal Transgenesis

Advanced Protocols for Animal Transgenesis

Author: Shirley Pease

Publisher: Springer Nature

Published: 2011-08-30

Total Pages: 671

ISBN-13: 3642207928

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This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.


Book Synopsis Advanced Protocols for Animal Transgenesis by : Shirley Pease

Download or read book Advanced Protocols for Animal Transgenesis written by Shirley Pease and published by Springer Nature. This book was released on 2011-08-30 with total page 671 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Advanced Protocols for Animal Transgenesis

Advanced Protocols for Animal Transgenesis

Author: Shirley Pease

Publisher: Springer

Published: 2016-08-23

Total Pages: 669

ISBN-13: 9783662519431

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This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.


Book Synopsis Advanced Protocols for Animal Transgenesis by : Shirley Pease

Download or read book Advanced Protocols for Animal Transgenesis written by Shirley Pease and published by Springer. This book was released on 2016-08-23 with total page 669 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Transgenic Animal Technology

Transgenic Animal Technology

Author: Carl A. Pinkert

Publisher: Academic Press

Published: 2012-12-02

Total Pages: 377

ISBN-13: 0323137830

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Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions, as well as to make practical applications to production agriculture. This well-illustrated handbook covers the technical aspects of gene transfer in animals--from molecular methods to whole animal considerations--for the important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Describes mouse model systems Covers laboratory and domestic animal species Illustrated with diagrams and photographs Includes useful tables and charts


Book Synopsis Transgenic Animal Technology by : Carl A. Pinkert

Download or read book Transgenic Animal Technology written by Carl A. Pinkert and published by Academic Press. This book was released on 2012-12-02 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions, as well as to make practical applications to production agriculture. This well-illustrated handbook covers the technical aspects of gene transfer in animals--from molecular methods to whole animal considerations--for the important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Describes mouse model systems Covers laboratory and domestic animal species Illustrated with diagrams and photographs Includes useful tables and charts

Guidelines On - Animal Use Protocol Review

Guidelines On - Animal Use Protocol Review

Author: Canadian Council on Animal Care

Publisher:

Published: 1998-05-01

Total Pages: 12

ISBN-13: 9780919087279

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Book Synopsis Guidelines On - Animal Use Protocol Review by : Canadian Council on Animal Care

Download or read book Guidelines On - Animal Use Protocol Review written by Canadian Council on Animal Care and published by . This book was released on 1998-05-01 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Welfare Aspects of Transgenic Animals

Welfare Aspects of Transgenic Animals

Author: L.F.M. van Zutphen

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 131

ISBN-13: 3642605893

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v Preface The quality of animal models used in biomedical research has increased substantially since the first successful microinjection of foreign DNA frag ments into a mouse zygote in the early 1980s. New well-defined animal models, developed either by micro injection techniques or by homologous recombination in embryonic stem (ES) cells, have become widely available and several classical animal models have now been replaced by these transgenic models. Transgenic animals also fulfill increasingly important roles in other fields, like livestock production or in biopharming. The potential benefits of this technology are overwhelming. It is not surprising that many research groups have rushed to jump on this wagon of scientific progress. However, no matter how important these developments are, they can not relieve us of the moral obligations towards the animals. It is generally recognized that animal use is allowed only if the harmful effects are out weighed by the potential benefits of the experimental results. But what are the harmful effects? What is the impact of trans genesis on the well-being of animals? In order to gain more insight into this aspect of trans genesis the European Commission (DGXII) has funded a 12-month project with the aim of col lecting information on the production and use of transgenic animals in EU Member States and, more specifically, to obtain information as to what extent specific welfare problems may arise and whether or not studies are being performed on the incidence of welfare problems.


Book Synopsis Welfare Aspects of Transgenic Animals by : L.F.M. van Zutphen

Download or read book Welfare Aspects of Transgenic Animals written by L.F.M. van Zutphen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt: v Preface The quality of animal models used in biomedical research has increased substantially since the first successful microinjection of foreign DNA frag ments into a mouse zygote in the early 1980s. New well-defined animal models, developed either by micro injection techniques or by homologous recombination in embryonic stem (ES) cells, have become widely available and several classical animal models have now been replaced by these transgenic models. Transgenic animals also fulfill increasingly important roles in other fields, like livestock production or in biopharming. The potential benefits of this technology are overwhelming. It is not surprising that many research groups have rushed to jump on this wagon of scientific progress. However, no matter how important these developments are, they can not relieve us of the moral obligations towards the animals. It is generally recognized that animal use is allowed only if the harmful effects are out weighed by the potential benefits of the experimental results. But what are the harmful effects? What is the impact of trans genesis on the well-being of animals? In order to gain more insight into this aspect of trans genesis the European Commission (DGXII) has funded a 12-month project with the aim of col lecting information on the production and use of transgenic animals in EU Member States and, more specifically, to obtain information as to what extent specific welfare problems may arise and whether or not studies are being performed on the incidence of welfare problems.

Animal Biotechnology

Animal Biotechnology

Author: National Research Council

Publisher: National Academies Press

Published: 2002-12-29

Total Pages: 201

ISBN-13: 0309084393

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Genetic-based animal biotechnology has produced new food and pharmaceutical products and promises many more advances to benefit humankind. These exciting prospects are accompanied by considerable unease, however, about matters such as safety and ethics. This book identifies science-based and policy-related concerns about animal biotechnologyâ€"key issues that must be resolved before the new breakthroughs can reach their potential. The book includes a short history of the field and provides understandable definitions of terms like cloning. Looking at technologies on the near horizon, the authors discuss what we know and what we fear about their effectsâ€"the inadvertent release of dangerous microorganisms, the safety of products derived from biotechnology, the impact of genetically engineered animals on their environment. In addition to these concerns, the book explores animal welfare concerns, and our societal and institutional capacity to manage and regulate the technology and its products. This accessible volume will be important to everyone interested in the implications of the use of animal biotechnology.


Book Synopsis Animal Biotechnology by : National Research Council

Download or read book Animal Biotechnology written by National Research Council and published by National Academies Press. This book was released on 2002-12-29 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic-based animal biotechnology has produced new food and pharmaceutical products and promises many more advances to benefit humankind. These exciting prospects are accompanied by considerable unease, however, about matters such as safety and ethics. This book identifies science-based and policy-related concerns about animal biotechnologyâ€"key issues that must be resolved before the new breakthroughs can reach their potential. The book includes a short history of the field and provides understandable definitions of terms like cloning. Looking at technologies on the near horizon, the authors discuss what we know and what we fear about their effectsâ€"the inadvertent release of dangerous microorganisms, the safety of products derived from biotechnology, the impact of genetically engineered animals on their environment. In addition to these concerns, the book explores animal welfare concerns, and our societal and institutional capacity to manage and regulate the technology and its products. This accessible volume will be important to everyone interested in the implications of the use of animal biotechnology.

Transgenic Animals in Agriculture

Transgenic Animals in Agriculture

Author: James Dickson Murray

Publisher: Cabi

Published: 1999

Total Pages: 312

ISBN-13:

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In the past decade, a number of advances have been made in genetic engineering as applied to farmed animals. This book has been developed from invited presentations at a conference held in California in August 1997, to address this issue. It is written by representatives from the leading laboratories involved in attempts to improve agriculturally important mammals, poultry and fish. Current knowledge, methodology, technical improvements and successes in the applications of transgenic technology to a range of animals which are important in agriculture are brought together for the first time under one cover. This book is essential reading for research workers in animal genetics, breeding and biotechnology.


Book Synopsis Transgenic Animals in Agriculture by : James Dickson Murray

Download or read book Transgenic Animals in Agriculture written by James Dickson Murray and published by Cabi. This book was released on 1999 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past decade, a number of advances have been made in genetic engineering as applied to farmed animals. This book has been developed from invited presentations at a conference held in California in August 1997, to address this issue. It is written by representatives from the leading laboratories involved in attempts to improve agriculturally important mammals, poultry and fish. Current knowledge, methodology, technical improvements and successes in the applications of transgenic technology to a range of animals which are important in agriculture are brought together for the first time under one cover. This book is essential reading for research workers in animal genetics, breeding and biotechnology.

Sustainable Food Production

Sustainable Food Production

Author: Paul Christou

Publisher: Springer

Published: 2012-12-05

Total Pages: 1869

ISBN-13: 9781461457961

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Gathering some 90 entries from the Encyclopedia of Sustainability Science and Technology, this book covers animal breeding and genetics for food, crop science and technology, ocean farming and sustainable aquaculture, transgenic livestock for food and more.


Book Synopsis Sustainable Food Production by : Paul Christou

Download or read book Sustainable Food Production written by Paul Christou and published by Springer. This book was released on 2012-12-05 with total page 1869 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gathering some 90 entries from the Encyclopedia of Sustainability Science and Technology, this book covers animal breeding and genetics for food, crop science and technology, ocean farming and sustainable aquaculture, transgenic livestock for food and more.

Transgenic Animals

Transgenic Animals

Author: Louis-Marie Houdebine

Publisher: CRC Press

Published: 2022-04-19

Total Pages: 122

ISBN-13: 1000448436

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During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues. Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics. Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.


Book Synopsis Transgenic Animals by : Louis-Marie Houdebine

Download or read book Transgenic Animals written by Louis-Marie Houdebine and published by CRC Press. This book was released on 2022-04-19 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues. Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics. Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.

Transgenic Animal Technology

Transgenic Animal Technology

Author: Carl A. Pinkert

Publisher: Newnes

Published: 2014-06-17

Total Pages: 715

ISBN-13: 0124095364

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Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Fully revised with updates to reflect state-of-the-art technology and associated changes to date


Book Synopsis Transgenic Animal Technology by : Carl A. Pinkert

Download or read book Transgenic Animal Technology written by Carl A. Pinkert and published by Newnes. This book was released on 2014-06-17 with total page 715 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Fully revised with updates to reflect state-of-the-art technology and associated changes to date