Affinity Labeling

Affinity Labeling

Author: William B. Jakoby

Publisher: Academic Press

Published: 1977-08-28

Total Pages: 808

ISBN-13:

DOWNLOAD EBOOK

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.


Book Synopsis Affinity Labeling by : William B. Jakoby

Download or read book Affinity Labeling written by William B. Jakoby and published by Academic Press. This book was released on 1977-08-28 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.


Theory and Practice in Affinity Techniques

Theory and Practice in Affinity Techniques

Author: P. V. Sundaram

Publisher:

Published: 1978

Total Pages: 264

ISBN-13:

DOWNLOAD EBOOK


Book Synopsis Theory and Practice in Affinity Techniques by : P. V. Sundaram

Download or read book Theory and Practice in Affinity Techniques written by P. V. Sundaram and published by . This book was released on 1978 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:


Affinity Labeling of Nucleotide Sites and Synthesis of a Novel Bifunctional Affinity Reagent

Affinity Labeling of Nucleotide Sites and Synthesis of a Novel Bifunctional Affinity Reagent

Author: James R. LaDine

Publisher:

Published: 1991

Total Pages: 114

ISBN-13:

DOWNLOAD EBOOK


Book Synopsis Affinity Labeling of Nucleotide Sites and Synthesis of a Novel Bifunctional Affinity Reagent by : James R. LaDine

Download or read book Affinity Labeling of Nucleotide Sites and Synthesis of a Novel Bifunctional Affinity Reagent written by James R. LaDine and published by . This book was released on 1991 with total page 114 pages. Available in PDF, EPUB and Kindle. Book excerpt:


Affinity Labeling Studies on Antibodies to a Polysaccaride Antigen and to a Carbohydrate Hapten

Affinity Labeling Studies on Antibodies to a Polysaccaride Antigen and to a Carbohydrate Hapten

Author: Isobel Contento Cornell

Publisher:

Published: 1969

Total Pages:

ISBN-13:

DOWNLOAD EBOOK


Book Synopsis Affinity Labeling Studies on Antibodies to a Polysaccaride Antigen and to a Carbohydrate Hapten by : Isobel Contento Cornell

Download or read book Affinity Labeling Studies on Antibodies to a Polysaccaride Antigen and to a Carbohydrate Hapten written by Isobel Contento Cornell and published by . This book was released on 1969 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:


Proximity Labeling

Proximity Labeling

Author: Murat Sunbul

Publisher: Springer Nature

Published: 2019-05-23

Total Pages: 231

ISBN-13: 1493995375

DOWNLOAD EBOOK

This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.


Book Synopsis Proximity Labeling by : Murat Sunbul

Download or read book Proximity Labeling written by Murat Sunbul and published by Springer Nature. This book was released on 2019-05-23 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.


Methods in Enzymology

Methods in Enzymology

Author: Nathan O. Kaplan

Publisher:

Published: 1977

Total Pages: 774

ISBN-13:

DOWNLOAD EBOOK


Book Synopsis Methods in Enzymology by : Nathan O. Kaplan

Download or read book Methods in Enzymology written by Nathan O. Kaplan and published by . This book was released on 1977 with total page 774 pages. Available in PDF, EPUB and Kindle. Book excerpt:


Affinity Modification Of Biopolymers

Affinity Modification Of Biopolymers

Author: Dmitri G Knorre

Publisher: CRC Press

Published: 2018-01-18

Total Pages: 277

ISBN-13: 1351078011

DOWNLOAD EBOOK

The goal of this book is to give a systematic description of the main principles of affinity modification and applications, consideration of possibilities, and restrictions of the method. Modification within specific complexes is a special case of chemical modification which is widely used in the nonaddressed version in biochemistry and related areas. Therefore, we have included in the first introductory paper chapter of the book general considerations of chemical modifications of biopolymers and the application of biopolymers.


Book Synopsis Affinity Modification Of Biopolymers by : Dmitri G Knorre

Download or read book Affinity Modification Of Biopolymers written by Dmitri G Knorre and published by CRC Press. This book was released on 2018-01-18 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of this book is to give a systematic description of the main principles of affinity modification and applications, consideration of possibilities, and restrictions of the method. Modification within specific complexes is a special case of chemical modification which is widely used in the nonaddressed version in biochemistry and related areas. Therefore, we have included in the first introductory paper chapter of the book general considerations of chemical modifications of biopolymers and the application of biopolymers.


A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis

A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis

Author: T. Meier

Publisher: Birkhäuser

Published: 1996-02

Total Pages: 264

ISBN-13:

DOWNLOAD EBOOK

2.1 Introduction ........................................................... 32 2.2 Technical Procedures ................................................... 32 Chemical Synthesis ..................................................... 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 38 . . . . . . . . . . . . . Chemical Synthesis ..................................................... 38 Binding to anti-CCK antisera and CCK-B receptors .......................... 40 Binding to streptavidin agarose and photoelution ........................... 40 Affinity chromatography ................................................. 41 2.4 Troubleshooting....................................................... 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ......................... 45 Summary ............................................................. 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures ................................................... 48 Preparation of a Biotinylated Ligand ...................................... 48 Preparation of Membrane Fraction ........................................ 50 Solubilization of the PACAP Receptor ..................................... 51 Partial Purification of the PACAP Receptor ................................. 52 Affinity Purification of the PACAP Receptor ................................ 54 Final Purification of the PACAP Receptor .................................. 56 Receptor-Binding Assay ................................................. 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting ....................................................... 61 Impurity in PACAP27-Cys-NH............................................ 61 2 Low specific activity of the PACAP receptor despite a single band ............. 62 Acknowledgments ..................................................... 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ......... " 65 Summary ............................................................. 65 4.1 Introduction................................................ . . . . . . . .. . 66 . 4.2 Technical Procedures ................................................. " 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ............. 67 Synthesis of photo reactive biotinylated peptide hormones ................... 67 Site-specific incorporation of biotin and photo labels in separate steps ........ 69 Photoaffinity labeling ................................................... 72 4.3 Results and Discussion .................................................. 76 Synthesis of photoactivatable insulins with permanent biotin labels ............ 76 Applications: Insulin .................................................... 78 VI Examples for other applications .......................................... 78 4.4 Troubleshooting....................................................... 79 References . . . . . . . . . . . . . . . . . . . . .


Book Synopsis A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis by : T. Meier

Download or read book A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis written by T. Meier and published by Birkhäuser. This book was released on 1996-02 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: 2.1 Introduction ........................................................... 32 2.2 Technical Procedures ................................................... 32 Chemical Synthesis ..................................................... 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 38 . . . . . . . . . . . . . Chemical Synthesis ..................................................... 38 Binding to anti-CCK antisera and CCK-B receptors .......................... 40 Binding to streptavidin agarose and photoelution ........................... 40 Affinity chromatography ................................................. 41 2.4 Troubleshooting....................................................... 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ......................... 45 Summary ............................................................. 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures ................................................... 48 Preparation of a Biotinylated Ligand ...................................... 48 Preparation of Membrane Fraction ........................................ 50 Solubilization of the PACAP Receptor ..................................... 51 Partial Purification of the PACAP Receptor ................................. 52 Affinity Purification of the PACAP Receptor ................................ 54 Final Purification of the PACAP Receptor .................................. 56 Receptor-Binding Assay ................................................. 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting ....................................................... 61 Impurity in PACAP27-Cys-NH............................................ 61 2 Low specific activity of the PACAP receptor despite a single band ............. 62 Acknowledgments ..................................................... 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ......... " 65 Summary ............................................................. 65 4.1 Introduction................................................ . . . . . . . .. . 66 . 4.2 Technical Procedures ................................................. " 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ............. 67 Synthesis of photo reactive biotinylated peptide hormones ................... 67 Site-specific incorporation of biotin and photo labels in separate steps ........ 69 Photoaffinity labeling ................................................... 72 4.3 Results and Discussion .................................................. 76 Synthesis of photoactivatable insulins with permanent biotin labels ............ 76 Applications: Insulin .................................................... 78 VI Examples for other applications .......................................... 78 4.4 Troubleshooting....................................................... 79 References . . . . . . . . . . . . . . . . . . . . .


Affinity Labelling and Cloning of Steroid and Thyroid Hormone Receptors

Affinity Labelling and Cloning of Steroid and Thyroid Hormone Receptors

Author: Hinrich Gronemeyer

Publisher:

Published: 1988

Total Pages: 336

ISBN-13:

DOWNLOAD EBOOK


Book Synopsis Affinity Labelling and Cloning of Steroid and Thyroid Hormone Receptors by : Hinrich Gronemeyer

Download or read book Affinity Labelling and Cloning of Steroid and Thyroid Hormone Receptors written by Hinrich Gronemeyer and published by . This book was released on 1988 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:


The Antibody Molecule

The Antibody Molecule

Author: Alfred Nisonoff

Publisher: Academic Press

Published: 2014-06-28

Total Pages: 560

ISBN-13: 1483273857

DOWNLOAD EBOOK

The Antibody Molecule reviews the literature leading to current knowledge of the structure of immunoglobulins. The book begins by outlining some of the basic structural characteristics of immunoglobulins without citing the references on which the information is based. Separate chapters follow covering the chemical nature of the active site of an antibody molecule and mechanisms of interaction with hapten; the general structural features and properties of the various classes of human immunoglobulin; and amino acid sequences of human and mouse L chains and of human and rabbit H chains. Subsequent chapters deal with the evolution of the immunoglobulin classes; special properties of mouse, guinea pig, rabbit, and horse immunoglobulins; idiotypic specificities of immunglobulins; and the genetic control of antibodies. This book is meant for immunologists who have not personally observed the development of this exciting period in the history of immunology. It will also provide useful supplemental reading for the serious student or investigator who wishes to become familiar with the nature of the antibody molecule, its genetic control, and mode of action.


Book Synopsis The Antibody Molecule by : Alfred Nisonoff

Download or read book The Antibody Molecule written by Alfred Nisonoff and published by Academic Press. This book was released on 2014-06-28 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Antibody Molecule reviews the literature leading to current knowledge of the structure of immunoglobulins. The book begins by outlining some of the basic structural characteristics of immunoglobulins without citing the references on which the information is based. Separate chapters follow covering the chemical nature of the active site of an antibody molecule and mechanisms of interaction with hapten; the general structural features and properties of the various classes of human immunoglobulin; and amino acid sequences of human and mouse L chains and of human and rabbit H chains. Subsequent chapters deal with the evolution of the immunoglobulin classes; special properties of mouse, guinea pig, rabbit, and horse immunoglobulins; idiotypic specificities of immunglobulins; and the genetic control of antibodies. This book is meant for immunologists who have not personally observed the development of this exciting period in the history of immunology. It will also provide useful supplemental reading for the serious student or investigator who wishes to become familiar with the nature of the antibody molecule, its genetic control, and mode of action.