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This report presents the results of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater.
Book Synopsis Application of DNA Microarray Technology for Wastewater Analysis by : Frédéric Berthiaume
Download or read book Application of DNA Microarray Technology for Wastewater Analysis written by Frédéric Berthiaume and published by International Water Assn. This book was released on 2004 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report presents the results of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater.
Microarray Technology Through Applications provides the reader with an understanding, from an applications perspective, of the diverse range of concepts required to master the experimental and data analysis aspects of microarray technology. The first chapter is a concise introduction to the technology and provides the theoretical background required to understand the subsequent sections. The following chapters are a series of case studies representative of the most general and important applications of microarray technology, including CGH, analysis of gene expression, SNP arrays and protein arrays. The case studies are written by experts in the field and describe prototypic projects, indicating how to generalize the approach to similar studies. There are detailed step-by-step protocols describing the specific experimental and data analysis protocols mentioned in the case study section. There is also information on printing glass DNA microarray slides and data interpretation. Colour figures and data sets are provided on the website at http://www.garlandscience.com/9780415378536
Book Synopsis Microarray Technology Through Applications by : Francesco Falciani
Download or read book Microarray Technology Through Applications written by Francesco Falciani and published by Garland Science. This book was released on 2007-06-30 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarray Technology Through Applications provides the reader with an understanding, from an applications perspective, of the diverse range of concepts required to master the experimental and data analysis aspects of microarray technology. The first chapter is a concise introduction to the technology and provides the theoretical background required to understand the subsequent sections. The following chapters are a series of case studies representative of the most general and important applications of microarray technology, including CGH, analysis of gene expression, SNP arrays and protein arrays. The case studies are written by experts in the field and describe prototypic projects, indicating how to generalize the approach to similar studies. There are detailed step-by-step protocols describing the specific experimental and data analysis protocols mentioned in the case study section. There is also information on printing glass DNA microarray slides and data interpretation. Colour figures and data sets are provided on the website at http://www.garlandscience.com/9780415378536
DNA microarray technology has become a useful technique in gene expression analysis for the development of new diagnostic tools and for the identification of disease genes and therapeutic targets for human cancers. Appropriate control for DNA microarray experiment and reliable analysis of the array data are key to performing the assay and utilizing the data correctly. The most difficult challenge has been the lack of a powerful method to analyze the data for all genes (more than 30,000 genes) simultaneously and to use the microarray data in a decision-making process. In this book, the authors describe DNA microarray technology and data analysis by pointing out current advantages and disadvantages of the technique and available analytical methods. Crucially, new ideas and analytical methods based on the authors' own experience in DNA microarray study and analysis are introduced. It is believed that this new way of interpreting and analyzing microarray data will bring us closer to success in decision-making using the information obtained through the DNA microarray technology.
Book Synopsis Dna Microarray Technology And Data Analysis In Cancer Research by : Shaoguang Li
Download or read book Dna Microarray Technology And Data Analysis In Cancer Research written by Shaoguang Li and published by World Scientific. This book was released on 2009-02-04 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA microarray technology has become a useful technique in gene expression analysis for the development of new diagnostic tools and for the identification of disease genes and therapeutic targets for human cancers. Appropriate control for DNA microarray experiment and reliable analysis of the array data are key to performing the assay and utilizing the data correctly. The most difficult challenge has been the lack of a powerful method to analyze the data for all genes (more than 30,000 genes) simultaneously and to use the microarray data in a decision-making process. In this book, the authors describe DNA microarray technology and data analysis by pointing out current advantages and disadvantages of the technique and available analytical methods. Crucially, new ideas and analytical methods based on the authors' own experience in DNA microarray study and analysis are introduced. It is believed that this new way of interpreting and analyzing microarray data will bring us closer to success in decision-making using the information obtained through the DNA microarray technology.
Ithasbeenstatedthatourknowledgedoublesevery20years,butthatmaybe an understatement when considering the Life Sciences. A series of discoveries and inventions have propelled our knowledge from the recognition that DNA isthegeneticmaterialtoabasicmolecularunderstandingofourselvesandthe living world around us in less than 50 years. Crucial to this rapid progress was thediscoveryofthedouble-helicalstructureofDNA,whichlaidthefoundation forallhybridizationbasedtechnologies. Thediscoveriesofrestrictionenzymes, ligases, polymerases, combined with key innovations in DNA synthesis and sequencing ushered in the era of biotechnologyas a new science with profound sociological and economic implications that are likely to have a dominating in?uence on the development of our society during this century. Given the process by which science builds on prior knowledge, it is perhaps unfair to single out a few inventions and credit them with having contributed most to thisavalancheofknowledge. Yet,therearesurelysomethatwillberecognized as having had a more profound impact than others, not just in the furthering of our scienti?c knowledge, but by leveraging commercial applications that provide a tangible return to our society. The now famous Polymerase Chain Reaction, or PCR, is surely one of those, as it has uniquely catalyzed molecular biology during the past 20 years, and continues to have a signi?cant impact on all areas that involve nucleic acids, ranging from molecular pathology to forensics. Ten years ago micro- ray technology emerged as a new and powerful tool to study nucleic acid - quences in a highly multiplexed manner, and has since found equally exciting and useful applications in the study of proteins, metabolites, toxins, viruses, whole cells and even tissues.
Book Synopsis Microarray Technology and Its Applications by : Uwe R. Müller
Download or read book Microarray Technology and Its Applications written by Uwe R. Müller and published by Springer Science & Business Media. This book was released on 2006-03-30 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ithasbeenstatedthatourknowledgedoublesevery20years,butthatmaybe an understatement when considering the Life Sciences. A series of discoveries and inventions have propelled our knowledge from the recognition that DNA isthegeneticmaterialtoabasicmolecularunderstandingofourselvesandthe living world around us in less than 50 years. Crucial to this rapid progress was thediscoveryofthedouble-helicalstructureofDNA,whichlaidthefoundation forallhybridizationbasedtechnologies. Thediscoveriesofrestrictionenzymes, ligases, polymerases, combined with key innovations in DNA synthesis and sequencing ushered in the era of biotechnologyas a new science with profound sociological and economic implications that are likely to have a dominating in?uence on the development of our society during this century. Given the process by which science builds on prior knowledge, it is perhaps unfair to single out a few inventions and credit them with having contributed most to thisavalancheofknowledge. Yet,therearesurelysomethatwillberecognized as having had a more profound impact than others, not just in the furthering of our scienti?c knowledge, but by leveraging commercial applications that provide a tangible return to our society. The now famous Polymerase Chain Reaction, or PCR, is surely one of those, as it has uniquely catalyzed molecular biology during the past 20 years, and continues to have a signi?cant impact on all areas that involve nucleic acids, ranging from molecular pathology to forensics. Ten years ago micro- ray technology emerged as a new and powerful tool to study nucleic acid - quences in a highly multiplexed manner, and has since found equally exciting and useful applications in the study of proteins, metabolites, toxins, viruses, whole cells and even tissues.
Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays—from their conception to their ready off-the-shelf availability—for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T .
Book Synopsis DNA Arrays by : Jang B. Rampal
Download or read book DNA Arrays written by Jang B. Rampal and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays—from their conception to their ready off-the-shelf availability—for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T .
Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique. Cohesive overview of the technology and available platforms, followed by detailed discussion of experimental design and analysis of microarray experiments Up-to-date description of normalization methods and current methods for sample amplification and labeling Deep focus on oligonucleotide design, printing, labeling and hybridization, data acquisition, normalization, and meta-analysis Additional uses of microarray technology such as ChIP (chromatin immunoprecipitation) with hybridization to DNA arrays, microarray-based comparative genomic hybridization (CGH), and cell and tissue arrays
Book Synopsis Microarray Technology in Practice by : Steve Russell
Download or read book Microarray Technology in Practice written by Steve Russell and published by Academic Press. This book was released on 2008-11-21 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique. Cohesive overview of the technology and available platforms, followed by detailed discussion of experimental design and analysis of microarray experiments Up-to-date description of normalization methods and current methods for sample amplification and labeling Deep focus on oligonucleotide design, printing, labeling and hybridization, data acquisition, normalization, and meta-analysis Additional uses of microarray technology such as ChIP (chromatin immunoprecipitation) with hybridization to DNA arrays, microarray-based comparative genomic hybridization (CGH), and cell and tissue arrays
The analysis of gene expression profile data from DNA micorarray studies are discussed in this book. It provides a review of available methods and presents it in a manner that is intelligible to biologists. It offers an understanding of the design and analysis of experiments utilizing microarrays to benefit scientists. It includes an Appendix tutorial on the use of BRB-ArrayTools and step by step analyses of several major datasets using this software which is available from the National Cancer Institute.
Book Synopsis Design and Analysis of DNA Microarray Investigations by : Richard M. Simon
Download or read book Design and Analysis of DNA Microarray Investigations written by Richard M. Simon and published by Springer Science & Business Media. This book was released on 2006-05-09 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis of gene expression profile data from DNA micorarray studies are discussed in this book. It provides a review of available methods and presents it in a manner that is intelligible to biologists. It offers an understanding of the design and analysis of experiments utilizing microarrays to benefit scientists. It includes an Appendix tutorial on the use of BRB-ArrayTools and step by step analyses of several major datasets using this software which is available from the National Cancer Institute.
Massive data acquisition technologies, such as genome sequencing, high-throughput drug screening, and DNA arrays are in the process of revolutionizing biology and medicine. Using the mRNA of a given cell, at a given time, under a given set of conditions, DNA microarrays can provide a snapshot of the level of expression of all the genes in the cell. Such snapshots can be used to study fundamental biological phenomena such as development or evolution, to determine the function of new genes, to infer the role individual genes or groups of genes may play in diseases, and to monitor the effect of drugs and other compounds on gene expression. Originally published in 2002, this inter-disciplinary introduction to DNA arrays will be of value to anyone with an a interest in this powerful technology.
Book Synopsis DNA Microarrays and Gene Expression by : Pierre Baldi
Download or read book DNA Microarrays and Gene Expression written by Pierre Baldi and published by Cambridge University Press. This book was released on 2011-04-28 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Massive data acquisition technologies, such as genome sequencing, high-throughput drug screening, and DNA arrays are in the process of revolutionizing biology and medicine. Using the mRNA of a given cell, at a given time, under a given set of conditions, DNA microarrays can provide a snapshot of the level of expression of all the genes in the cell. Such snapshots can be used to study fundamental biological phenomena such as development or evolution, to determine the function of new genes, to infer the role individual genes or groups of genes may play in diseases, and to monitor the effect of drugs and other compounds on gene expression. Originally published in 2002, this inter-disciplinary introduction to DNA arrays will be of value to anyone with an a interest in this powerful technology.
DNA Microarrays: Methods Express covers the very latest in DNA microarray technology, with a clear focus on how these techniques can be used in the lab to gain the very best results. The authors are from some of the leading laboratories in the field and write with real authority on the latest methodology. Every chapter provides detailed step-by-step protocols with valuable hints and tips for success, as well as giving typical experimental results and selected literature citations. This book is a 'must have' manual for researchers in all fields of biology, medicine and agriculture.
Book Synopsis DNA Microarrays by : Mark Schena
Download or read book DNA Microarrays written by Mark Schena and published by Scion Publishing Ltd. This book was released on 2007-10-01 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA Microarrays: Methods Express covers the very latest in DNA microarray technology, with a clear focus on how these techniques can be used in the lab to gain the very best results. The authors are from some of the leading laboratories in the field and write with real authority on the latest methodology. Every chapter provides detailed step-by-step protocols with valuable hints and tips for success, as well as giving typical experimental results and selected literature citations. This book is a 'must have' manual for researchers in all fields of biology, medicine and agriculture.
Wastewater Treatment: Molecular Tools, Techniques, and Applications provides an insight about the application of different tools and technology for exploring microbial structure-function relationships that involved in WWTPs. From the present day consequence of alarming usable water crysis throughout the globe, an immediate action on water cycle is necessary. Along with other options the waste water recycling is one major opportunity to combat the future scarcity. The book aims to provide a comprehensive view of advanced emerging technologies for wastewater treatment, heavy metal removal, pesticide degradation, dye removal, waste management, microbial transformation of environmental contaminants, etc. It also describes different application of Omic tools in Waste water treatment plants (WWTPs),describes the role of microorganisms in WWTPs, points out the reuse of treated wastewater through emerging technologies, also includes the recovery of resources from wastewater and emphasizes on cutting edge molecular tools for WWTPs. We hope the content of the book will be very much usefull for the community who are directly associated in wastewater management research, people who are associated with environmental awarness programme and the students of UG and PG courses. Features: This book highlights the importance of molecular genomics, molecular biology techniques to sort out the problems faced by industrialist who operates wastewater treatment plant with the ever-increasing number of environmental pollutants. Describes application of different Omic tools in Wastewater treatment plants (WWTPs) Describes the role of microorganisms in WWTPs Points out the reuse of treated wastewater through emerging technologies. Includes the recovery of resources from wastewater Emphasizes on cutting edge molecular tools This book targets engineers, scientists and managers who require an excellent introduction and basic knowledge to the principles of molecular biology or molecular genomics in the area of wastewater treatment. Different professionals working or interested in the Environmental Microbiology or Bioremediation or Environmental Genomics field. Students on Environmental Biotechnology/Microbiology.
Book Synopsis Wastewater Treatment by : Maulin P. Shah
Download or read book Wastewater Treatment written by Maulin P. Shah and published by CRC Press. This book was released on 2022-02-16 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wastewater Treatment: Molecular Tools, Techniques, and Applications provides an insight about the application of different tools and technology for exploring microbial structure-function relationships that involved in WWTPs. From the present day consequence of alarming usable water crysis throughout the globe, an immediate action on water cycle is necessary. Along with other options the waste water recycling is one major opportunity to combat the future scarcity. The book aims to provide a comprehensive view of advanced emerging technologies for wastewater treatment, heavy metal removal, pesticide degradation, dye removal, waste management, microbial transformation of environmental contaminants, etc. It also describes different application of Omic tools in Waste water treatment plants (WWTPs),describes the role of microorganisms in WWTPs, points out the reuse of treated wastewater through emerging technologies, also includes the recovery of resources from wastewater and emphasizes on cutting edge molecular tools for WWTPs. We hope the content of the book will be very much usefull for the community who are directly associated in wastewater management research, people who are associated with environmental awarness programme and the students of UG and PG courses. Features: This book highlights the importance of molecular genomics, molecular biology techniques to sort out the problems faced by industrialist who operates wastewater treatment plant with the ever-increasing number of environmental pollutants. Describes application of different Omic tools in Wastewater treatment plants (WWTPs) Describes the role of microorganisms in WWTPs Points out the reuse of treated wastewater through emerging technologies. Includes the recovery of resources from wastewater Emphasizes on cutting edge molecular tools This book targets engineers, scientists and managers who require an excellent introduction and basic knowledge to the principles of molecular biology or molecular genomics in the area of wastewater treatment. Different professionals working or interested in the Environmental Microbiology or Bioremediation or Environmental Genomics field. Students on Environmental Biotechnology/Microbiology.
