Applications of Raman Spectroscopy to Biology

Applications of Raman Spectroscopy to Biology

Author: M. Ghomi

Publisher: IOS Press

Published: 2012-02-23

Total Pages: 204

ISBN-13: 1614990204

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Raman spectroscopy has been known and used as a technique for 80 years, originally for the study of inorganic substances. Recent advances in underlying technology, such as lasers, detectors, filters and components, have transformed the technique into a very effective modern tool for studying complex biological problems. Professor Mahmoud Ghomi (of the University of Paris XIII) has edited this book on the applications of Raman spectroscopy to biology, covering in a readily accessible way the area from basic studies to the diagnosis of disease. The early chapters provide background information on basic principles underlying the main Raman methods covered in the book, with information on Surface-Enhanced Raman Scattering (SERS) and Surface-Enhanced Fluorescence (SEF), as well as giving accounts of applications to biomolecular and cellular investigations. Among the topics covered are studies of drugs and their complexes with biomolecules on nanoparticles, application of SERS to blood analysis, studies of single cells and of applications to human cancer diagnostics.This will be a useful book for experimental scientists in academic, governmental, industrial and clinical environments and for those entering the field of biomolecular spectroscopy.


Book Synopsis Applications of Raman Spectroscopy to Biology by : M. Ghomi

Download or read book Applications of Raman Spectroscopy to Biology written by M. Ghomi and published by IOS Press. This book was released on 2012-02-23 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Raman spectroscopy has been known and used as a technique for 80 years, originally for the study of inorganic substances. Recent advances in underlying technology, such as lasers, detectors, filters and components, have transformed the technique into a very effective modern tool for studying complex biological problems. Professor Mahmoud Ghomi (of the University of Paris XIII) has edited this book on the applications of Raman spectroscopy to biology, covering in a readily accessible way the area from basic studies to the diagnosis of disease. The early chapters provide background information on basic principles underlying the main Raman methods covered in the book, with information on Surface-Enhanced Raman Scattering (SERS) and Surface-Enhanced Fluorescence (SEF), as well as giving accounts of applications to biomolecular and cellular investigations. Among the topics covered are studies of drugs and their complexes with biomolecules on nanoparticles, application of SERS to blood analysis, studies of single cells and of applications to human cancer diagnostics.This will be a useful book for experimental scientists in academic, governmental, industrial and clinical environments and for those entering the field of biomolecular spectroscopy.


Raman Spectroscopy in Biology

Raman Spectroscopy in Biology

Author: Anthony T. Tu

Publisher: John Wiley & Sons

Published: 1982

Total Pages: 472

ISBN-13:

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Book Synopsis Raman Spectroscopy in Biology by : Anthony T. Tu

Download or read book Raman Spectroscopy in Biology written by Anthony T. Tu and published by John Wiley & Sons. This book was released on 1982 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt:


Biochemical Applications of Raman and Resonance Raman Spectroscopes

Biochemical Applications of Raman and Resonance Raman Spectroscopes

Author: P Carey

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 277

ISBN-13: 0323158129

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Biochemical Applications of Raman and Resonance Raman Spectroscopies focuses on the application of Raman and resonance Raman spectroscopies to biochemical problems. The book reviews biological systems and details the application of Raman spectroscopy to biological molecules such as proteins, nucleic acids, and lipids. It also looks at codevelopments of lasers, optics, and electronics that drive advances in experimental Raman spectroscopy, along with the important ramifications of these advances for biochemical applications. This volume is organized into eight chapters and begins with an overview of the theoretical and experimental aspects of Raman spectroscopy, including a very brief explanation of what Raman and resonance Raman spectroscopies are and a discussion of their advantages and disadvantages for biochemical studies. The explanation of the Raman and resonance Raman effects is taken up in more detail in the next chapter, which develops the concept of the vibrational motions of molecules by initially considering mechanical ""ball and spring"" models and goes on to use this concept to formulate a classical model for Raman scattering. The resonance Raman effect is then described by another model which emphasizes the discrete or quantized energy levels available to a molecule. The reader is also introduced to the experimental aspects of Raman spectroscopy and the application of Raman spectroscopy across the entire field of biochemistry. Each chapter contains an outline of the basic chemistry and biochemical nomenclature involved. This book will be of interest to chemists, biochemists, and spectroscopists, as well as graduate students and experienced research workers.


Book Synopsis Biochemical Applications of Raman and Resonance Raman Spectroscopes by : P Carey

Download or read book Biochemical Applications of Raman and Resonance Raman Spectroscopes written by P Carey and published by Elsevier. This book was released on 2012-12-02 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemical Applications of Raman and Resonance Raman Spectroscopies focuses on the application of Raman and resonance Raman spectroscopies to biochemical problems. The book reviews biological systems and details the application of Raman spectroscopy to biological molecules such as proteins, nucleic acids, and lipids. It also looks at codevelopments of lasers, optics, and electronics that drive advances in experimental Raman spectroscopy, along with the important ramifications of these advances for biochemical applications. This volume is organized into eight chapters and begins with an overview of the theoretical and experimental aspects of Raman spectroscopy, including a very brief explanation of what Raman and resonance Raman spectroscopies are and a discussion of their advantages and disadvantages for biochemical studies. The explanation of the Raman and resonance Raman effects is taken up in more detail in the next chapter, which develops the concept of the vibrational motions of molecules by initially considering mechanical ""ball and spring"" models and goes on to use this concept to formulate a classical model for Raman scattering. The resonance Raman effect is then described by another model which emphasizes the discrete or quantized energy levels available to a molecule. The reader is also introduced to the experimental aspects of Raman spectroscopy and the application of Raman spectroscopy across the entire field of biochemistry. Each chapter contains an outline of the basic chemistry and biochemical nomenclature involved. This book will be of interest to chemists, biochemists, and spectroscopists, as well as graduate students and experienced research workers.


Raman Spectroscopy in Biology

Raman Spectroscopy in Biology

Author: Anthony T. Tu

Publisher:

Published: 1982-01-01

Total Pages: 464

ISBN-13: 9780783728032

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Book Synopsis Raman Spectroscopy in Biology by : Anthony T. Tu

Download or read book Raman Spectroscopy in Biology written by Anthony T. Tu and published by . This book was released on 1982-01-01 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:


Raman Spectroscopy and Applications

Raman Spectroscopy and Applications

Author: Khan Maaz

Publisher: BoD – Books on Demand

Published: 2017-02-15

Total Pages: 366

ISBN-13: 9535129074

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Raman spectroscopy has a number of applications in various fields including material science, physics, chemistry, biology, geology, and medicine. This book illustrates necessary insight and guidance in the field of Raman spectroscopy with detailed figures and explanations. This presents deep understanding of new techniques from basic introduction to the advance level for scientists and engineers. The chapters cover all major aspects of Raman spectroscopy and its application in material characterization with special emphasis on both the theoretical and experimental aspects. This book is aimed to provide solid foundation of Raman spectroscopy to the students, scientists, and engineers working in various fields as mentioned above.


Book Synopsis Raman Spectroscopy and Applications by : Khan Maaz

Download or read book Raman Spectroscopy and Applications written by Khan Maaz and published by BoD – Books on Demand. This book was released on 2017-02-15 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: Raman spectroscopy has a number of applications in various fields including material science, physics, chemistry, biology, geology, and medicine. This book illustrates necessary insight and guidance in the field of Raman spectroscopy with detailed figures and explanations. This presents deep understanding of new techniques from basic introduction to the advance level for scientists and engineers. The chapters cover all major aspects of Raman spectroscopy and its application in material characterization with special emphasis on both the theoretical and experimental aspects. This book is aimed to provide solid foundation of Raman spectroscopy to the students, scientists, and engineers working in various fields as mentioned above.


Applications of Infrared, Raman, and Resonance Raman Spectroscopy in Biochemistry

Applications of Infrared, Raman, and Resonance Raman Spectroscopy in Biochemistry

Author: Frank S. Parker

Publisher: Springer Science & Business Media

Published: 1983-10-01

Total Pages: 582

ISBN-13: 9780306412066

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Book Synopsis Applications of Infrared, Raman, and Resonance Raman Spectroscopy in Biochemistry by : Frank S. Parker

Download or read book Applications of Infrared, Raman, and Resonance Raman Spectroscopy in Biochemistry written by Frank S. Parker and published by Springer Science & Business Media. This book was released on 1983-10-01 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt:


Biological Applications of Raman Spectroscopy

Biological Applications of Raman Spectroscopy

Author: Thomas G. Spiro

Publisher: John Wiley & Sons

Published: 1988-02

Total Pages: 1324

ISBN-13: 9780471815723

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Book Synopsis Biological Applications of Raman Spectroscopy by : Thomas G. Spiro

Download or read book Biological Applications of Raman Spectroscopy written by Thomas G. Spiro and published by John Wiley & Sons. This book was released on 1988-02 with total page 1324 pages. Available in PDF, EPUB and Kindle. Book excerpt:


Raman Spectroscopy and its Application in Nanostructures

Raman Spectroscopy and its Application in Nanostructures

Author: Shu-Lin Zhang

Publisher: John Wiley & Sons

Published: 2012-01-24

Total Pages: 519

ISBN-13: 1119966787

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Raman Spectroscopy and its Application in Nanostructures is an original and timely contribution to a very active area of physics and materials science research. This book presents the theoretical and experimental phenomena of Raman spectroscopy, with specialized discussions on the physical fundamentals, new developments and main features in low-dimensional systems of Raman spectroscopy. In recent years physicists, materials scientists and chemists have devoted increasing attention to low-dimensional systems and as Raman spectroscopy can be used to study and analyse such materials as carbon nanotubes, quantum wells, silicon nanowires, etc., it is fast becoming one of the most powerful and sensitive experimental techniques to characterize the qualities of such nanostructures. Recent scientific and technological developments have resulted in the applications of Raman spectroscopy to expand. These developments are vital in providing information for a very broad field of applications: for example in microelectronics, biology, forensics and archaeology. Thus, this book not only introduces these important new branches of Raman spectroscopy from both a theoretical and practical view point, but the resulting effects are fully explored and relevant representative models of Raman spectra are described in-depth with the inclusion of theoretical calculations, when appropriate.


Book Synopsis Raman Spectroscopy and its Application in Nanostructures by : Shu-Lin Zhang

Download or read book Raman Spectroscopy and its Application in Nanostructures written by Shu-Lin Zhang and published by John Wiley & Sons. This book was released on 2012-01-24 with total page 519 pages. Available in PDF, EPUB and Kindle. Book excerpt: Raman Spectroscopy and its Application in Nanostructures is an original and timely contribution to a very active area of physics and materials science research. This book presents the theoretical and experimental phenomena of Raman spectroscopy, with specialized discussions on the physical fundamentals, new developments and main features in low-dimensional systems of Raman spectroscopy. In recent years physicists, materials scientists and chemists have devoted increasing attention to low-dimensional systems and as Raman spectroscopy can be used to study and analyse such materials as carbon nanotubes, quantum wells, silicon nanowires, etc., it is fast becoming one of the most powerful and sensitive experimental techniques to characterize the qualities of such nanostructures. Recent scientific and technological developments have resulted in the applications of Raman spectroscopy to expand. These developments are vital in providing information for a very broad field of applications: for example in microelectronics, biology, forensics and archaeology. Thus, this book not only introduces these important new branches of Raman spectroscopy from both a theoretical and practical view point, but the resulting effects are fully explored and relevant representative models of Raman spectra are described in-depth with the inclusion of theoretical calculations, when appropriate.


Microfluidics for Single-Cell Analysis

Microfluidics for Single-Cell Analysis

Author: Jin-Ming Lin

Publisher: Springer Nature

Published: 2019-08-28

Total Pages: 261

ISBN-13: 9813297298

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This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.


Book Synopsis Microfluidics for Single-Cell Analysis by : Jin-Ming Lin

Download or read book Microfluidics for Single-Cell Analysis written by Jin-Ming Lin and published by Springer Nature. This book was released on 2019-08-28 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.


Surface-Enhanced Raman Spectroscopy

Surface-Enhanced Raman Spectroscopy

Author: Marek Prochazka

Publisher: Springer

Published: 2015-12-12

Total Pages: 221

ISBN-13: 3319239929

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This book gives an overview of recent developments in RS and SERS for sensing and biosensing considering also limitations, possibilities and prospects of this technique. Raman scattering (RS) is a widely used vibrational technique providing highly specific molecular spectral patterns. A severe limitation for the application of this spectroscopic technique lies in the low cross section of RS. Surface-enhanced Raman scattering (SERS) spectroscopy overcomes this problem by 6-11 orders of magnitude enhancement compared with the standard RS for molecules in the close vicinity of certain rough metal surfaces. Thus, SERS combines molecular fingerprint specificity with potential single-molecule sensitivity. Due to the recent development of new SERS-active substrates, labeling and derivatization chemistry as well as new instrumentations, SERS became a very promising tool for many varied applications, including bioanalytical studies and sensing. Both intrinsic and extrinsic SERS biosensing schemes have been employed to detect and identify small molecules, nucleic acids and proteins, and also for cellular and in vivo sensing.


Book Synopsis Surface-Enhanced Raman Spectroscopy by : Marek Prochazka

Download or read book Surface-Enhanced Raman Spectroscopy written by Marek Prochazka and published by Springer. This book was released on 2015-12-12 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives an overview of recent developments in RS and SERS for sensing and biosensing considering also limitations, possibilities and prospects of this technique. Raman scattering (RS) is a widely used vibrational technique providing highly specific molecular spectral patterns. A severe limitation for the application of this spectroscopic technique lies in the low cross section of RS. Surface-enhanced Raman scattering (SERS) spectroscopy overcomes this problem by 6-11 orders of magnitude enhancement compared with the standard RS for molecules in the close vicinity of certain rough metal surfaces. Thus, SERS combines molecular fingerprint specificity with potential single-molecule sensitivity. Due to the recent development of new SERS-active substrates, labeling and derivatization chemistry as well as new instrumentations, SERS became a very promising tool for many varied applications, including bioanalytical studies and sensing. Both intrinsic and extrinsic SERS biosensing schemes have been employed to detect and identify small molecules, nucleic acids and proteins, and also for cellular and in vivo sensing.