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The Baculoviruses

Author: Lois K. Miller

Publisher: Springer Science & Business Media

Published: 2013-06-29

Total Pages: 459

ISBN-13: 1489918345

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The past decade has witnessed an explosion of information on the molecular biology of insect viruses and a frenzy of activity in applying this information to medicine and agriculture. Genetically engineered baculoviruses are presently being tested for commercial use as pesticides, and the study of such viruses is also revealing remarkable insights into basic cellular processes such as apoptosis. This comprehensive volume provides readers with knowledge of basic and applied baculovirology so that current literature in the field can be appreciated.

Book Synopsis The Baculoviruses by : Lois K. Miller

Download or read book The Baculoviruses written by Lois K. Miller and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has witnessed an explosion of information on the molecular biology of insect viruses and a frenzy of activity in applying this information to medicine and agriculture. Genetically engineered baculoviruses are presently being tested for commercial use as pesticides, and the study of such viruses is also revealing remarkable insights into basic cellular processes such as apoptosis. This comprehensive volume provides readers with knowledge of basic and applied baculovirology so that current literature in the field can be appreciated.

BIOLOGY OF BACULOVIRUSES

Author: Robert R. Granados

Publisher: Springer

Published: 1986

Total Pages: 296

ISBN-13:

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V.1 - Biological properties and molecular biology: Historical perspectives; Taxonomy and identification of baculoviruses; Ultrastructure of baculoviruses; In vivo infection and replication of baculoviruses; In vitro replication of baculoviruses; Baculovirus structural proteins and protein synthesis; Current developments in baculovirus serology; Persistent baculovirus infections; Specificity and safety of baculoviruses; Evolution of occluded baculoviruses; The genetics of baculoviruses; Organization and expression of the baculovirus genome; v.2 - Practical application for insect control: In vivo and in vitro bioassay methods for baculoviruses; In vivo production of baculoviruses; Cell culture methods for large-scale propagation of baculoviruses; Ecology and ens, chemical pesticides, and parasitoids; Formulation and application of baculoviruses; Use of baculoviruses in pest management programs; Registration of baculoviruses as pesticides; Practical factors influencing the utilization of baculoviruses as pesticides; Insect resistance to baculoviruses.

Book Synopsis BIOLOGY OF BACULOVIRUSES by : Robert R. Granados

Download or read book BIOLOGY OF BACULOVIRUSES written by Robert R. Granados and published by Springer. This book was released on 1986 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: V.1 - Biological properties and molecular biology: Historical perspectives; Taxonomy and identification of baculoviruses; Ultrastructure of baculoviruses; In vivo infection and replication of baculoviruses; In vitro replication of baculoviruses; Baculovirus structural proteins and protein synthesis; Current developments in baculovirus serology; Persistent baculovirus infections; Specificity and safety of baculoviruses; Evolution of occluded baculoviruses; The genetics of baculoviruses; Organization and expression of the baculovirus genome; v.2 - Practical application for insect control: In vivo and in vitro bioassay methods for baculoviruses; In vivo production of baculoviruses; Cell culture methods for large-scale propagation of baculoviruses; Ecology and ens, chemical pesticides, and parasitoids; Formulation and application of baculoviruses; Use of baculoviruses in pest management programs; Registration of baculoviruses as pesticides; Practical factors influencing the utilization of baculoviruses as pesticides; Insect resistance to baculoviruses.

Baculovirus and Insect Cell Expression Protocols

Author: David W. Murhammer

Publisher: Methods in Molecular Biology

Published: 2018-04-15

Total Pages: 522

ISBN-13: 9781493979783

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The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

Book Synopsis Baculovirus and Insect Cell Expression Protocols by : David W. Murhammer

Download or read book Baculovirus and Insect Cell Expression Protocols written by David W. Murhammer and published by Methods in Molecular Biology. This book was released on 2018-04-15 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

Insect Viruses

Author: Byrony C Bonning

Publisher: Academic Press

Published: 2006-09-22

Total Pages: 513

ISBN-13: 0120398680

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Baculoviruses are perhaps unique among viruses in the breadth of their biotechnological applications: these insect specific viruses are used not only for insect pest management purposes, but also as laboratory research tools for production of recombinant proteins and for protein display, and as potential vectors for human gene therapy. In addition to highlighting recent advances, this volume provides a comprehensive review of the biotechnological applications of these and other insect viruses in both the academic and private sectors.

Book Synopsis Insect Viruses by : Byrony C Bonning

Download or read book Insect Viruses written by Byrony C Bonning and published by Academic Press. This book was released on 2006-09-22 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: Baculoviruses are perhaps unique among viruses in the breadth of their biotechnological applications: these insect specific viruses are used not only for insect pest management purposes, but also as laboratory research tools for production of recombinant proteins and for protein display, and as potential vectors for human gene therapy. In addition to highlighting recent advances, this volume provides a comprehensive review of the biotechnological applications of these and other insect viruses in both the academic and private sectors.

Insect Cell Culture Engineering

Author: Mattheus F. A. Goosen

Publisher: CRC Press

Published: 2020-07-24

Total Pages: 260

ISBN-13: 1000105660

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Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.

Book Synopsis Insect Cell Culture Engineering by : Mattheus F. A. Goosen

Download or read book Insect Cell Culture Engineering written by Mattheus F. A. Goosen and published by CRC Press. This book was released on 2020-07-24 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.

BIOLOGY OF BACULOVIRUSES

Author: Robert R. Granados

Publisher: Springer

Published: 1986

Total Pages: 294

ISBN-13:

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Book Synopsis BIOLOGY OF BACULOVIRUSES by : Robert R. Granados

Download or read book BIOLOGY OF BACULOVIRUSES written by Robert R. Granados and published by Springer. This book was released on 1986 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Viral Insecticides for Biological Control

Author: Karl Maramorosch

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 823

ISBN-13: 0323142370

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Viral Insecticides for Biological Control focuses on the basic as well as applied aspects of viral insecticides, which have the potential to significantly reduce the current reliance on chemical pesticide technology. This book serves as a guide for the development of means to identify hazardous problems and prevent them. Organized into six parts with a total of 23 chapters, this book describes the taxonomy, nomenclature, identification, physical, biological, as well as chemical characteristics, replication, and pathology of insect viruses. This reference material also explores the dispersal, stability, and utilization of insect viruses as biological control agents. The factors and considerations that must be taken into account when a viral insecticide is sought as a large-scale commercial alternative to other more traditional methods of pest control are also addressed. Because this reference material collates information in this field of interest, it will benefit a wide audience of readers, including researchers, students, and those working directly in crop protection.

Book Synopsis Viral Insecticides for Biological Control by : Karl Maramorosch

Download or read book Viral Insecticides for Biological Control written by Karl Maramorosch and published by Elsevier. This book was released on 2012-12-02 with total page 823 pages. Available in PDF, EPUB and Kindle. Book excerpt: Viral Insecticides for Biological Control focuses on the basic as well as applied aspects of viral insecticides, which have the potential to significantly reduce the current reliance on chemical pesticide technology. This book serves as a guide for the development of means to identify hazardous problems and prevent them. Organized into six parts with a total of 23 chapters, this book describes the taxonomy, nomenclature, identification, physical, biological, as well as chemical characteristics, replication, and pathology of insect viruses. This reference material also explores the dispersal, stability, and utilization of insect viruses as biological control agents. The factors and considerations that must be taken into account when a viral insecticide is sought as a large-scale commercial alternative to other more traditional methods of pest control are also addressed. Because this reference material collates information in this field of interest, it will benefit a wide audience of readers, including researchers, students, and those working directly in crop protection.

Baculovirus Expression Vectors

Author: David R. O'Reilly

Publisher:

Published: 1994

Total Pages: 366

ISBN-13: 9780195091311

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Baculoviruses have proven to be the most powerful and versatile eukaryotic expression vectors available. This unique laboratory manual is designed to help both beginning and experienced researchers construct and use baculovirus vector systems. It simplifies selection of the most appropriatebaculovirus vector design for a given problem, then describes each step of the implementation process--from vector construction to large-scale protein production. The book provides an understanding of how the vectors work; a biological overview of cells, viruses, plasmids, and promoters; guidelinesfor choosing optimum vectors; protocols for growing insect cells and recombinant viruses; methods of analyzing protein products and scaling up protein production; techniques for producing proteins in insect larvae; and easy-to-use maps charting available expression vectors. This comprehensiveapproach has many benefits for researchers and students alike. It allows them to understand how and why the vector system works and offers a rapid comparison of options for choosing the right virus, plasmid or promoter for vector design and construction, with a minimum amount of lost time. Themanual is an invaluable resource for every individual engaged in the production of proteins for any purpose.

Book Synopsis Baculovirus Expression Vectors by : David R. O'Reilly

Download or read book Baculovirus Expression Vectors written by David R. O'Reilly and published by . This book was released on 1994 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: Baculoviruses have proven to be the most powerful and versatile eukaryotic expression vectors available. This unique laboratory manual is designed to help both beginning and experienced researchers construct and use baculovirus vector systems. It simplifies selection of the most appropriatebaculovirus vector design for a given problem, then describes each step of the implementation process--from vector construction to large-scale protein production. The book provides an understanding of how the vectors work; a biological overview of cells, viruses, plasmids, and promoters; guidelinesfor choosing optimum vectors; protocols for growing insect cells and recombinant viruses; methods of analyzing protein products and scaling up protein production; techniques for producing proteins in insect larvae; and easy-to-use maps charting available expression vectors. This comprehensiveapproach has many benefits for researchers and students alike. It allows them to understand how and why the vector system works and offers a rapid comparison of options for choosing the right virus, plasmid or promoter for vector design and construction, with a minimum amount of lost time. Themanual is an invaluable resource for every individual engaged in the production of proteins for any purpose.

Baculovirus Expression Systems and Biopesticides

Author: Michael L. Shuler

Publisher: Wiley-Liss

Published: 1995

Total Pages: 280

ISBN-13:

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Developing effective baculovirus-insect culture systems. Insect cell culture methods and their use in virus research. Comparison of mammalian and insect cell cultures. protein production and processing from baculovirus. Development and testing of genetically improved baculovirus insecticides. Fundamentals of baculovirus-insect cell attachment and infection. Development and evaluation of host insect cells. Bioreactor design and scale-up issues. The effect of hydrodynamic forces on insect cells. Commercial application of insect cell culture. Baculovirus-mediated production of proteins in insect cells. Potential application of insect cell-based expression systems in the bio/pharmaceutical industry.

Book Synopsis Baculovirus Expression Systems and Biopesticides by : Michael L. Shuler

Download or read book Baculovirus Expression Systems and Biopesticides written by Michael L. Shuler and published by Wiley-Liss. This book was released on 1995 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developing effective baculovirus-insect culture systems. Insect cell culture methods and their use in virus research. Comparison of mammalian and insect cell cultures. protein production and processing from baculovirus. Development and testing of genetically improved baculovirus insecticides. Fundamentals of baculovirus-insect cell attachment and infection. Development and evaluation of host insect cells. Bioreactor design and scale-up issues. The effect of hydrodynamic forces on insect cells. Commercial application of insect cell culture. Baculovirus-mediated production of proteins in insect cells. Potential application of insect cell-based expression systems in the bio/pharmaceutical industry.

Biological Control of Pest and Vector Insects

Author: Vonnie D.C. Shields

Publisher: BoD – Books on Demand

Published: 2017-04-05

Total Pages: 362

ISBN-13: 9535130358

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This book provides recent contributions of current strategies to control insect pests written by experts in their respective fields. Topics include semiochemicals based insect management techniques, assessment of lethal dose/concentrations, strategies for efficient biological control practices, bioinsecticidal formulations and mechanisms of action involving RNAi technology, light-trap collection of insects, the use of sex pheromonal components and attractants for pest insect capture, measures to increase plant resistance in forest plantations, the use of various baculoviruses as biopesticides, and effect of a pathogenic bacterium against an endangered butterfly species. There are several other chapters that focus on insect vectors, including biting midges as livestock vectors in Tunisia, mosquitoes as vectors in Brazil, human disease vectors in Tanzania, pathogenic livestock and human vectors in Africa, insect vectors of Chagas disease, and transgenic and paratransgenic biotechnologies against dipteran pests and vectors. This book targets general biologists, entomologists, ecologists, zoologists, virologists, and epidemiologists, including both teachers and students.

Book Synopsis Biological Control of Pest and Vector Insects by : Vonnie D.C. Shields

Download or read book Biological Control of Pest and Vector Insects written by Vonnie D.C. Shields and published by BoD – Books on Demand. This book was released on 2017-04-05 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides recent contributions of current strategies to control insect pests written by experts in their respective fields. Topics include semiochemicals based insect management techniques, assessment of lethal dose/concentrations, strategies for efficient biological control practices, bioinsecticidal formulations and mechanisms of action involving RNAi technology, light-trap collection of insects, the use of sex pheromonal components and attractants for pest insect capture, measures to increase plant resistance in forest plantations, the use of various baculoviruses as biopesticides, and effect of a pathogenic bacterium against an endangered butterfly species. There are several other chapters that focus on insect vectors, including biting midges as livestock vectors in Tunisia, mosquitoes as vectors in Brazil, human disease vectors in Tanzania, pathogenic livestock and human vectors in Africa, insect vectors of Chagas disease, and transgenic and paratransgenic biotechnologies against dipteran pests and vectors. This book targets general biologists, entomologists, ecologists, zoologists, virologists, and epidemiologists, including both teachers and students.