Calcium-Binding Protein Protocols

Calcium-Binding Protein Protocols

Author: Hans J. Vogel

Publisher: Springer Science & Business Media

Published: 2008-02-04

Total Pages: 346

ISBN-13: 1592591833

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Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.


Book Synopsis Calcium-Binding Protein Protocols by : Hans J. Vogel

Download or read book Calcium-Binding Protein Protocols written by Hans J. Vogel and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.


Calcium-binding Protein Protocols

Calcium-binding Protein Protocols

Author: Hans J. Vogel

Publisher:

Published: 2002

Total Pages: 0

ISBN-13:

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This handbook focuses on modern experimental analyses and methodologies for the study of calcium-binding proteins.


Book Synopsis Calcium-binding Protein Protocols by : Hans J. Vogel

Download or read book Calcium-binding Protein Protocols written by Hans J. Vogel and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook focuses on modern experimental analyses and methodologies for the study of calcium-binding proteins.


Calcium-Binding Protein Protocols

Calcium-Binding Protein Protocols

Author: Hans J. Vogel

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 415

ISBN-13: 1592591841

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Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.


Book Synopsis Calcium-Binding Protein Protocols by : Hans J. Vogel

Download or read book Calcium-Binding Protein Protocols written by Hans J. Vogel and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.


Calcium-Binding Proteins of the EF-Hand Superfamily

Calcium-Binding Proteins of the EF-Hand Superfamily

Author: Claus W. Heizmann

Publisher:

Published: 2019

Total Pages: 779

ISBN-13: 9781493990306

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This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry.


Book Synopsis Calcium-Binding Proteins of the EF-Hand Superfamily by : Claus W. Heizmann

Download or read book Calcium-Binding Proteins of the EF-Hand Superfamily written by Claus W. Heizmann and published by . This book was released on 2019 with total page 779 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry.


Calcium-Binding Proteins of the EF-Hand Superfamily

Calcium-Binding Proteins of the EF-Hand Superfamily

Author: Claus W. Heizmann

Publisher: Humana Press

Published: 2019-02-02

Total Pages: 779

ISBN-13: 9781493990290

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This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry.


Book Synopsis Calcium-Binding Proteins of the EF-Hand Superfamily by : Claus W. Heizmann

Download or read book Calcium-Binding Proteins of the EF-Hand Superfamily written by Claus W. Heizmann and published by Humana Press. This book was released on 2019-02-02 with total page 779 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry.


Calcium Binding Proteins

Calcium Binding Proteins

Author: Eugene Permyakov

Publisher: John Wiley & Sons

Published: 2011-03-21

Total Pages: 456

ISBN-13: 1118099540

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Calcium Binding Proteins explains the unique and highly diverse functions of calcium in biology, which are realized by calcium binding proteins. The structures and physical characteristics of these calcium binding proteins are described, as well as their functions and general patterns of their evolution. Techniques that underlie the description of proteins are discussed, including NMR, circular dichroism, optical rotatory dispersion spectroscopy, calorimetry,and crystallography. The book discusses the patterns of bochmical phenomena such as calcium homeostasis, mineralization, and cell signaling that involve specific proteins. It summarizes ongoing research and presents general hypotheses that help to focus future research, and also provides a conceptual framework and a description of the underlying techniques that permits someone entering the field to become conversant.


Book Synopsis Calcium Binding Proteins by : Eugene Permyakov

Download or read book Calcium Binding Proteins written by Eugene Permyakov and published by John Wiley & Sons. This book was released on 2011-03-21 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calcium Binding Proteins explains the unique and highly diverse functions of calcium in biology, which are realized by calcium binding proteins. The structures and physical characteristics of these calcium binding proteins are described, as well as their functions and general patterns of their evolution. Techniques that underlie the description of proteins are discussed, including NMR, circular dichroism, optical rotatory dispersion spectroscopy, calorimetry,and crystallography. The book discusses the patterns of bochmical phenomena such as calcium homeostasis, mineralization, and cell signaling that involve specific proteins. It summarizes ongoing research and presents general hypotheses that help to focus future research, and also provides a conceptual framework and a description of the underlying techniques that permits someone entering the field to become conversant.


Calcium-Binding Proteins and Rage

Calcium-Binding Proteins and Rage

Author: Claus Heizmann

Publisher: Humana Press

Published: 2016-05-01

Total Pages: 440

ISBN-13: 9781493959761

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This Methods in Molecular Biology book reviews common techniques for studying calcium binding proteins, underlining the diagnostic and clinical importance of this family of proteins. Offers materials lists, protocols, troubleshooting advice and more."


Book Synopsis Calcium-Binding Proteins and Rage by : Claus Heizmann

Download or read book Calcium-Binding Proteins and Rage written by Claus Heizmann and published by Humana Press. This book was released on 2016-05-01 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Methods in Molecular Biology book reviews common techniques for studying calcium binding proteins, underlining the diagnostic and clinical importance of this family of proteins. Offers materials lists, protocols, troubleshooting advice and more."


Calcium and Calcium Binding Proteins

Calcium and Calcium Binding Proteins

Author: Charles Gerday

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 265

ISBN-13: 3642730426

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The idea of Professors Bolis and Gilles to gather together for a 3 days' meeting in the splendid environment of Crans-Montana in Switzerland a limited number of people around the subject of calcium and calcium bind ing proteins seemed at first particularly attractive, and when they asked me to take charge of the scientific organization of the symposium, I accepted with enthusiasm. It rapidly became clear that the major problem would be the selection of the topics, since it was impossible to cover completely and in depth such a broad and dynamic area of research. In our view, one imperative was to associate as intimately as possible the structural and the functional aspects of the areas covered. Apart from one whole day focused on the fascinating roles played by calmodulin in cellular activities, the other sessions were devoted to calmodulin-related calcium binding proteins in muscle and non muscle tissues and to some selected biological systems such as mitochondria, secretory cells or sarcoplasmic reticulum in which calcium also plays a crucial role. The presentations were made by leading investigators in their field. Some of them do not, however, appear in the present volume, for which there are two reasons: first, some of the contributions were somewhat outside the scope of the book; second, three speakers, for valid reasons, simply found no opportunity to write a manuscript in the allotted time.


Book Synopsis Calcium and Calcium Binding Proteins by : Charles Gerday

Download or read book Calcium and Calcium Binding Proteins written by Charles Gerday and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: The idea of Professors Bolis and Gilles to gather together for a 3 days' meeting in the splendid environment of Crans-Montana in Switzerland a limited number of people around the subject of calcium and calcium bind ing proteins seemed at first particularly attractive, and when they asked me to take charge of the scientific organization of the symposium, I accepted with enthusiasm. It rapidly became clear that the major problem would be the selection of the topics, since it was impossible to cover completely and in depth such a broad and dynamic area of research. In our view, one imperative was to associate as intimately as possible the structural and the functional aspects of the areas covered. Apart from one whole day focused on the fascinating roles played by calmodulin in cellular activities, the other sessions were devoted to calmodulin-related calcium binding proteins in muscle and non muscle tissues and to some selected biological systems such as mitochondria, secretory cells or sarcoplasmic reticulum in which calcium also plays a crucial role. The presentations were made by leading investigators in their field. Some of them do not, however, appear in the present volume, for which there are two reasons: first, some of the contributions were somewhat outside the scope of the book; second, three speakers, for valid reasons, simply found no opportunity to write a manuscript in the allotted time.


Calcium-binding Proteins

Calcium-binding Proteins

Author: Marvin P. Thompson

Publisher: C R C Press Reprints

Published: 1988

Total Pages: 168

ISBN-13:

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Book Synopsis Calcium-binding Proteins by : Marvin P. Thompson

Download or read book Calcium-binding Proteins written by Marvin P. Thompson and published by C R C Press Reprints. This book was released on 1988 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:


Calcium-Binding Proteins in Health and Disease

Calcium-Binding Proteins in Health and Disease

Author: Anthony Norman

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 650

ISBN-13: 0323141420

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Calcium-Binding Proteins in Health and Disease includes the papers prepared by the invited speakers as well as many of the free communications. The contributions are grouped according to their general subject matter, based on the classification made by the authors, e.g., Extracellular Metabolism: Calcium Homeostasis; Low-Affinity Calcium-Binding Proteins. There can be little doubt that calcium-binding proteins is a field of scientific endeavor which will continue to produce results of great interest to modern biology. This book is divided into eight sections, the first of which, tackle extracellular calcium metabolism or calcium homeostasis. The next sections focus on topics such as low-affinity calcium-binding proteins; calcium and membranes, channels and transport (pumps); the role of calcium in complex metabolic processes; and gene structure of calcium-binding proteins and their expression. Other chapters cover physical studies on calcium-binding proteins, including X-ray, crystallography, and NMR; structure-function relationships of calcium-binding proteins and their targets; and calcium-binding proteins in health and disease. This book will be of interest to practitioners in the fields of biology and medicine.


Book Synopsis Calcium-Binding Proteins in Health and Disease by : Anthony Norman

Download or read book Calcium-Binding Proteins in Health and Disease written by Anthony Norman and published by Elsevier. This book was released on 2012-12-02 with total page 650 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calcium-Binding Proteins in Health and Disease includes the papers prepared by the invited speakers as well as many of the free communications. The contributions are grouped according to their general subject matter, based on the classification made by the authors, e.g., Extracellular Metabolism: Calcium Homeostasis; Low-Affinity Calcium-Binding Proteins. There can be little doubt that calcium-binding proteins is a field of scientific endeavor which will continue to produce results of great interest to modern biology. This book is divided into eight sections, the first of which, tackle extracellular calcium metabolism or calcium homeostasis. The next sections focus on topics such as low-affinity calcium-binding proteins; calcium and membranes, channels and transport (pumps); the role of calcium in complex metabolic processes; and gene structure of calcium-binding proteins and their expression. Other chapters cover physical studies on calcium-binding proteins, including X-ray, crystallography, and NMR; structure-function relationships of calcium-binding proteins and their targets; and calcium-binding proteins in health and disease. This book will be of interest to practitioners in the fields of biology and medicine.