Calcium Measurement Methods

Calcium Measurement Methods

Author: Alexei Verkhratsky

Publisher: Humana Press

Published: 2012-02-25

Total Pages: 256

ISBN-13: 9781617796777

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Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signaling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In "Calcium Measurement Methods", a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book's contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful NeuromethodsTM series, the chapters provide authoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field. Cutting-edge and concise, "Calcium Measurement Methods" serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.


Book Synopsis Calcium Measurement Methods by : Alexei Verkhratsky

Download or read book Calcium Measurement Methods written by Alexei Verkhratsky and published by Humana Press. This book was released on 2012-02-25 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signaling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In "Calcium Measurement Methods", a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book's contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful NeuromethodsTM series, the chapters provide authoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field. Cutting-edge and concise, "Calcium Measurement Methods" serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.

Lectins and Glycobiology

Lectins and Glycobiology

Author: Hans-Joachim Gabius

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 524

ISBN-13: 3642779441

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The intriguing complexity, precision, and regulation of the wide range of biologi cal processes is determined by intricate mechanisms of molecular recognition. Their nature is under intense scrutiny. In addition to the well-appreciated interac tion of proteins either with amino acid or nucleotide sequences, the investigation of their interplay with carbohydrate elements of cellular glycoconjugates current ly exerts increasing attraction. In the group of carbohydrate-binding proteins, lec tins are distinguished from antibodies or ligand-affecting enzymes, according to the most recent definition. The thorough analysis of their structure and function is considered as a focus to collect a critical mass of information for delineating details of a further array of biochemical processes with pivotal physiological im pact. Following an already century-long history of scientific description, reflected by subjectively chosen highlights (see the Brief History of Lectin Research at page VI), the excitement in glycobiological research that prevails today can easily be ex plained by our growing awareness of the multifarious significance of a sugar-code system of biological information. This present notion unmistakably has an im pact on lines of research in diverse disciplines like cell and molecular biology, histochemistry, or clinical sciences. It also prompts inherent practical questions such as how to obtain lectins, or how to employ them as instruments in various assay systems with the best possible results. Thus, this book is devoted intentionally to cover the techniques in different research fields that deal with lectins.


Book Synopsis Lectins and Glycobiology by : Hans-Joachim Gabius

Download or read book Lectins and Glycobiology written by Hans-Joachim Gabius and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 524 pages. Available in PDF, EPUB and Kindle. Book excerpt: The intriguing complexity, precision, and regulation of the wide range of biologi cal processes is determined by intricate mechanisms of molecular recognition. Their nature is under intense scrutiny. In addition to the well-appreciated interac tion of proteins either with amino acid or nucleotide sequences, the investigation of their interplay with carbohydrate elements of cellular glycoconjugates current ly exerts increasing attraction. In the group of carbohydrate-binding proteins, lec tins are distinguished from antibodies or ligand-affecting enzymes, according to the most recent definition. The thorough analysis of their structure and function is considered as a focus to collect a critical mass of information for delineating details of a further array of biochemical processes with pivotal physiological im pact. Following an already century-long history of scientific description, reflected by subjectively chosen highlights (see the Brief History of Lectin Research at page VI), the excitement in glycobiological research that prevails today can easily be ex plained by our growing awareness of the multifarious significance of a sugar-code system of biological information. This present notion unmistakably has an im pact on lines of research in diverse disciplines like cell and molecular biology, histochemistry, or clinical sciences. It also prompts inherent practical questions such as how to obtain lectins, or how to employ them as instruments in various assay systems with the best possible results. Thus, this book is devoted intentionally to cover the techniques in different research fields that deal with lectins.

Standard Reference Materials

Standard Reference Materials

Author: J. Paul Cali

Publisher:

Published: 1972

Total Pages: 144

ISBN-13:

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Book Synopsis Standard Reference Materials by : J. Paul Cali

Download or read book Standard Reference Materials written by J. Paul Cali and published by . This book was released on 1972 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Calcium Transport Elements in Plants

Calcium Transport Elements in Plants

Author: Santosh Kumar Upadhyay

Publisher: Academic Press

Published: 2021-01-08

Total Pages: 494

ISBN-13: 0128217936

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Calcium Transport Elements in Plants discusses the role of calcium in plant development and stress signaling, the mechanism of Ca2+ homeostasis across plant membranes, and the evolution of Ca2+/cation antiporter (CaCA) superfamily proteins. Additional sections cover genome-wide analysis of Annexins and their roles in plants, the roles of calmodulin in abiotic stress responses, calcium transport in relation to plant nutrition/biofortification, and much more. Written by leading experts in the field, this title is an essential resource for students and researchers that need all of the information on calcium transport elements in one place. Calcium transport elements are involved in various structural, physiological and biochemical processes or signal transduction pathways in response to various abiotic and biotic stimuli. Development of high throughput sequencing technology has favored the identification and characterization of numerous gene families in plants in recent years, including the calcium transport elements. Provides a complete compilation of detailed information on Ca2+ efflux and influx transporters in plants Discusses the mode of action of calcium transport elements and their classification Explores the indispensable role of Ca2+ in numerous developmental and stress related pathways


Book Synopsis Calcium Transport Elements in Plants by : Santosh Kumar Upadhyay

Download or read book Calcium Transport Elements in Plants written by Santosh Kumar Upadhyay and published by Academic Press. This book was released on 2021-01-08 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calcium Transport Elements in Plants discusses the role of calcium in plant development and stress signaling, the mechanism of Ca2+ homeostasis across plant membranes, and the evolution of Ca2+/cation antiporter (CaCA) superfamily proteins. Additional sections cover genome-wide analysis of Annexins and their roles in plants, the roles of calmodulin in abiotic stress responses, calcium transport in relation to plant nutrition/biofortification, and much more. Written by leading experts in the field, this title is an essential resource for students and researchers that need all of the information on calcium transport elements in one place. Calcium transport elements are involved in various structural, physiological and biochemical processes or signal transduction pathways in response to various abiotic and biotic stimuli. Development of high throughput sequencing technology has favored the identification and characterization of numerous gene families in plants in recent years, including the calcium transport elements. Provides a complete compilation of detailed information on Ca2+ efflux and influx transporters in plants Discusses the mode of action of calcium transport elements and their classification Explores the indispensable role of Ca2+ in numerous developmental and stress related pathways

Calcium Entry Channels in Non-Excitable Cells

Calcium Entry Channels in Non-Excitable Cells

Author: Juliusz Ashot Kozak

Publisher: CRC Press

Published: 2017-07-14

Total Pages: 343

ISBN-13: 149875273X

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Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.


Book Synopsis Calcium Entry Channels in Non-Excitable Cells by : Juliusz Ashot Kozak

Download or read book Calcium Entry Channels in Non-Excitable Cells written by Juliusz Ashot Kozak and published by CRC Press. This book was released on 2017-07-14 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.

Calcium Stable Isotope Geochemistry

Calcium Stable Isotope Geochemistry

Author: Nikolaus Gussone

Publisher: Springer

Published: 2016-05-02

Total Pages: 260

ISBN-13: 3540689532

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This book provides an overview of the fundamentals and reference values for Ca stable isotope research, as well as current analytical methodologies including detailed instructions for sample preparation and isotope analysis. As such, it introduces readers to the different fields of application, including low-temperature mineral precipitation and biomineralisation, Earth surface processes and global cycling, high-temperature processes and cosmochemistry, and lastly human studies and biomedical applications. The current state of the art in these major areas is discussed, and open questions and possible future directions are identified. In terms of its depth and coverage, the current work extends and complements the previous reviews of Ca stable isotope geochemistry, addressing the needs of graduate students and advanced researchers who want to familiarize themselves with Ca stable isotope research.


Book Synopsis Calcium Stable Isotope Geochemistry by : Nikolaus Gussone

Download or read book Calcium Stable Isotope Geochemistry written by Nikolaus Gussone and published by Springer. This book was released on 2016-05-02 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an overview of the fundamentals and reference values for Ca stable isotope research, as well as current analytical methodologies including detailed instructions for sample preparation and isotope analysis. As such, it introduces readers to the different fields of application, including low-temperature mineral precipitation and biomineralisation, Earth surface processes and global cycling, high-temperature processes and cosmochemistry, and lastly human studies and biomedical applications. The current state of the art in these major areas is discussed, and open questions and possible future directions are identified. In terms of its depth and coverage, the current work extends and complements the previous reviews of Ca stable isotope geochemistry, addressing the needs of graduate students and advanced researchers who want to familiarize themselves with Ca stable isotope research.

Blood Clotting Enzymology

Blood Clotting Enzymology

Author: Walter H. Seegers

Publisher: Academic Press

Published: 2013-10-22

Total Pages: 641

ISBN-13: 1483225453

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Blood Clotting Enzymology covers the mechanisms of blood clotting and their role in thrombosis, hemostasis, and many associated ramifications. This book is composed of 12 chapters that consider the integration of knowledge on blood clotting enzymology with hemostasis physiology. The opening chapter deals with the enzymology of the sol-gel transformation was studied in terms of proteins, fats, carbohydrates, surface physics, immunology, biophysical mechanisms, structural architecture, vitamin K and its antagonists, and the rheology of the blood. The subsequent chapters review the biophysical properties and composition of active substance in blood coagulation, as well as the concepts of prothrombin activation. These topics are followed by discussions on the chemical and physicochemical properties of fibrinogen and fibrin; the application of immulogical methods to delineate important properties of plasma constituents; and the mechanisms of antithrombin activity. Other chapters tackle the importance of hirudin and cobra venom for certain experimental laboratory work in the study of the physiology of blood coagulation. The final chapters survey the role of platelets in hemostasis, bleeding disorders, and the chemistry and function of vitamin K in blood coagulation. This book will prove useful to hematologists, enzyme scientist, immunologists, and research workers who are interested in blood coagulation mechanisms.


Book Synopsis Blood Clotting Enzymology by : Walter H. Seegers

Download or read book Blood Clotting Enzymology written by Walter H. Seegers and published by Academic Press. This book was released on 2013-10-22 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: Blood Clotting Enzymology covers the mechanisms of blood clotting and their role in thrombosis, hemostasis, and many associated ramifications. This book is composed of 12 chapters that consider the integration of knowledge on blood clotting enzymology with hemostasis physiology. The opening chapter deals with the enzymology of the sol-gel transformation was studied in terms of proteins, fats, carbohydrates, surface physics, immunology, biophysical mechanisms, structural architecture, vitamin K and its antagonists, and the rheology of the blood. The subsequent chapters review the biophysical properties and composition of active substance in blood coagulation, as well as the concepts of prothrombin activation. These topics are followed by discussions on the chemical and physicochemical properties of fibrinogen and fibrin; the application of immulogical methods to delineate important properties of plasma constituents; and the mechanisms of antithrombin activity. Other chapters tackle the importance of hirudin and cobra venom for certain experimental laboratory work in the study of the physiology of blood coagulation. The final chapters survey the role of platelets in hemostasis, bleeding disorders, and the chemistry and function of vitamin K in blood coagulation. This book will prove useful to hematologists, enzyme scientist, immunologists, and research workers who are interested in blood coagulation mechanisms.

A Practical Guide to the Study of Calcium in Living Cells

A Practical Guide to the Study of Calcium in Living Cells

Author:

Publisher: Academic Press

Published: 1994-04-25

Total Pages: 393

ISBN-13: 0080859380

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A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes Fluorescent dyes Luminescent proteins Includes techniques for perturbing intracellular Ca2+ Covers detailed methodology plus problems and pitfalls of each technique Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program Color plates illustrate techniques such as Confocal ratio-imaging Use of aequorin


Book Synopsis A Practical Guide to the Study of Calcium in Living Cells by :

Download or read book A Practical Guide to the Study of Calcium in Living Cells written by and published by Academic Press. This book was released on 1994-04-25 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes Fluorescent dyes Luminescent proteins Includes techniques for perturbing intracellular Ca2+ Covers detailed methodology plus problems and pitfalls of each technique Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program Color plates illustrate techniques such as Confocal ratio-imaging Use of aequorin

Calcium in Living Cells

Calcium in Living Cells

Author:

Publisher: Academic Press

Published: 2010-11-26

Total Pages: 328

ISBN-13: 9780080951225

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Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases. Gives researchers much needed information on how to study calcium in live cells, which is becoming increasingly important in heart, musculoskeletal, and immune system research Provides an overview of the latest methods--fluorescence resonance energy transfer (FRET), for example-- that are new to the field Allows understanding of how calcium plays a role in intracellular function at the cellular level, which has proved important in Alzheimer's research, heart disease, and areas of musculoskeletal research Updated chapters reflect advancements in the classic techniques used'preparing calcium buffers, vibrating the Ca2+ Electrode and confocal imaging


Book Synopsis Calcium in Living Cells by :

Download or read book Calcium in Living Cells written by and published by Academic Press. This book was released on 2010-11-26 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases. Gives researchers much needed information on how to study calcium in live cells, which is becoming increasingly important in heart, musculoskeletal, and immune system research Provides an overview of the latest methods--fluorescence resonance energy transfer (FRET), for example-- that are new to the field Allows understanding of how calcium plays a role in intracellular function at the cellular level, which has proved important in Alzheimer's research, heart disease, and areas of musculoskeletal research Updated chapters reflect advancements in the classic techniques used'preparing calcium buffers, vibrating the Ca2+ Electrode and confocal imaging

Calcium Signaling Protocols

Calcium Signaling Protocols

Author: David G. Lambert

Publisher: Springer Science & Business Media

Published: 2008-02-03

Total Pages: 351

ISBN-13: 1592592503

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2+ The regulation of intracellular Ca is a common theme presented in many 2+ papers over the last 20 or so years, and the description of the Ca -sensitive indicator dye fura 2 in 1985 resulted in a massive increase in these types of 2+ studies. Aspects of the regulation of intracellular Ca have been dealt with in many of the subsequent chapters and will therefore not be covered again. Calcium Signaling Protocols results from a chance discussion with Dr. R. I. Norman of the Department of Medicine at Leicester University and r- resents a major effort from a group of extremely helpful and very patient - thors. Putting a book like this together takes time and I am indebted to these authors without whom this project would have remained a chance discussion. I am also very grateful to Professor J. M. Walker, the series editor, for all his help and advice over the course of this project and particularly his help editing the first batch of chapters. I would also like to thank Dr E. L. Pallett for help and advice regarding interconversion of Mac and Word files and for archiving chapters.


Book Synopsis Calcium Signaling Protocols by : David G. Lambert

Download or read book Calcium Signaling Protocols written by David G. Lambert and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: 2+ The regulation of intracellular Ca is a common theme presented in many 2+ papers over the last 20 or so years, and the description of the Ca -sensitive indicator dye fura 2 in 1985 resulted in a massive increase in these types of 2+ studies. Aspects of the regulation of intracellular Ca have been dealt with in many of the subsequent chapters and will therefore not be covered again. Calcium Signaling Protocols results from a chance discussion with Dr. R. I. Norman of the Department of Medicine at Leicester University and r- resents a major effort from a group of extremely helpful and very patient - thors. Putting a book like this together takes time and I am indebted to these authors without whom this project would have remained a chance discussion. I am also very grateful to Professor J. M. Walker, the series editor, for all his help and advice over the course of this project and particularly his help editing the first batch of chapters. I would also like to thank Dr E. L. Pallett for help and advice regarding interconversion of Mac and Word files and for archiving chapters.