Ecophysiology and Biochemistry of Cyanobacteria

Ecophysiology and Biochemistry of Cyanobacteria

Author: Rajesh Prasad Rastogi

Publisher: Springer Nature

Published: 2022-01-30

Total Pages: 485

ISBN-13: 9811648735

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This book emphasizes and presents the latest information on eco-physiology and biochemistry of cyanobacteria with special emphasis on their biodiversity, molecular mechanisms of some important biological processes and survival mechanisms under myriad of environmental conditions as well as bioremediation. Cyanobacteria are the most dominant prokaryotic floras on the Earth’s surface, and are of great importance in terms of ecological, economical and evolutionary perspectives. They are oldest groups of photosynthetic autotrophs, which create oxygenic atmosphere for the development and sustainability of ecosystems with different life forms. The book presents an integrative approach to their possible biotechnological application in the field of bio-energy and various aspects of biochemistry, biophysics and structural biology of photosynthesis. The various chapters describe the different applications of cyanobacteria as bio-energy sources and in phycoremediation. The contents incorporated in this book can be used as a textbook by undergraduate and post-graduate students, teachers, and researchers in the most interesting fields of physicochemical ecology and biochemistry of cyanobacteria.


Book Synopsis Ecophysiology and Biochemistry of Cyanobacteria by : Rajesh Prasad Rastogi

Download or read book Ecophysiology and Biochemistry of Cyanobacteria written by Rajesh Prasad Rastogi and published by Springer Nature. This book was released on 2022-01-30 with total page 485 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book emphasizes and presents the latest information on eco-physiology and biochemistry of cyanobacteria with special emphasis on their biodiversity, molecular mechanisms of some important biological processes and survival mechanisms under myriad of environmental conditions as well as bioremediation. Cyanobacteria are the most dominant prokaryotic floras on the Earth’s surface, and are of great importance in terms of ecological, economical and evolutionary perspectives. They are oldest groups of photosynthetic autotrophs, which create oxygenic atmosphere for the development and sustainability of ecosystems with different life forms. The book presents an integrative approach to their possible biotechnological application in the field of bio-energy and various aspects of biochemistry, biophysics and structural biology of photosynthesis. The various chapters describe the different applications of cyanobacteria as bio-energy sources and in phycoremediation. The contents incorporated in this book can be used as a textbook by undergraduate and post-graduate students, teachers, and researchers in the most interesting fields of physicochemical ecology and biochemistry of cyanobacteria.

The Ecology of Cyanobacteria

The Ecology of Cyanobacteria

Author: B.A. Whitton

Publisher: Springer Science & Business Media

Published: 2007-05-08

Total Pages: 718

ISBN-13: 0306468557

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Cyanobacteria make a major contribution to world photosynthesis and nitrogen fixation, but are also notorious for causing nuisances such as dense and often toxic `blooms' in lakes and the ocean. The Ecology of Cyanobacteria: Their Diversity in Time and Space is the first book to focus solely on ecological aspects of these organisms. Its twenty-two chapters are written by some thirty authors, who are leading experts in their particular subject. The book begins with an overview of the cyanobacteria - or blue-green algae, for those who are not specialists - then looks at their diversity in the geological record and goes on to describe their ecology in present environments where they play important roles. Why is one of the key groups of organisms in the Precambrian still one of the most important groups of phototrophs today? The importance of ecological information for rational management and exploitation of these organisms for commercial and other practical purposes is also assessed. Accounts are provided of nuisances as well as the ecology of the commercially successful Spirulina and the role of cyanobacteria in ecosystem recovery from oil pollution. Many chapters include aspects of physiology, biochemistry, geochemistry and molecular biology where these help general understanding of the subject. In addition there are three chapters dealing specifically with molecular ecology. Thirty-two pages of colour photos incorporate about seventy views and light micrographs. These features make the book valuable to a wide readership, including biologists, microbiologists, geologists, water managers and environmental consultants. The book complements the highly successful The Molecular Biology of Cyanobacteria already published by Kluwer.


Book Synopsis The Ecology of Cyanobacteria by : B.A. Whitton

Download or read book The Ecology of Cyanobacteria written by B.A. Whitton and published by Springer Science & Business Media. This book was released on 2007-05-08 with total page 718 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanobacteria make a major contribution to world photosynthesis and nitrogen fixation, but are also notorious for causing nuisances such as dense and often toxic `blooms' in lakes and the ocean. The Ecology of Cyanobacteria: Their Diversity in Time and Space is the first book to focus solely on ecological aspects of these organisms. Its twenty-two chapters are written by some thirty authors, who are leading experts in their particular subject. The book begins with an overview of the cyanobacteria - or blue-green algae, for those who are not specialists - then looks at their diversity in the geological record and goes on to describe their ecology in present environments where they play important roles. Why is one of the key groups of organisms in the Precambrian still one of the most important groups of phototrophs today? The importance of ecological information for rational management and exploitation of these organisms for commercial and other practical purposes is also assessed. Accounts are provided of nuisances as well as the ecology of the commercially successful Spirulina and the role of cyanobacteria in ecosystem recovery from oil pollution. Many chapters include aspects of physiology, biochemistry, geochemistry and molecular biology where these help general understanding of the subject. In addition there are three chapters dealing specifically with molecular ecology. Thirty-two pages of colour photos incorporate about seventy views and light micrographs. These features make the book valuable to a wide readership, including biologists, microbiologists, geologists, water managers and environmental consultants. The book complements the highly successful The Molecular Biology of Cyanobacteria already published by Kluwer.

Stress Biology of Cyanobacteria

Stress Biology of Cyanobacteria

Author: Ashish Kumar Srivastava

Publisher: CRC Press

Published: 2013-03-01

Total Pages: 397

ISBN-13: 1466504781

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A significant component of many different ecosystems, cyanobacteria occupy almost every niche of the earth, including fresh and salt waters, rice fields, hot springs, arid deserts, and polar regions. Cyanobacteria, along with algae, produce nearly half the global oxygen, making assessment of their ecophysiologies important for understanding climate impacts and potential remediation. Stress Biology of Cyanobacteria: Molecular Mechanisms to Cellular Responses is a compilation of holistic responses of cyanobacteria, ranging from ecological and physiological to the modern aspects of their molecular biology, genomics, and biochemistry. Covering almost every aspect of cyanobacterial stress biology, this book is divided into two parts: Bioenergetics and Molecular Mechanisms of Stress Tolerance and Cellular Responses and Ecophysiology. The first few chapters focus on the molecular bioenergetics of photosynthesis and respiration in cyanobacteria, and provide a clear perspective on different stress tolerance mechanisms. Part I also covers the effect of specific stresses—including heavy metal, high and low temperature, salt, osmotic, and UV-B stress—on a wide range of vital physiological, biochemical, and molecular processes of cyanobacteria. Part II describes mechanisms of symbiosis, stress-induced bioproducts, and the role of environmental factors on nitrogen fixation, which along with photosynthesis is a major contributor to the current geochemical status of the planet. The text also covers mutation and cyanobacterial adaptation, and the most widely studied cyanotoxin, microcystin, which has effects on both human and animal health. With contributions from experts around the world, representing the global importance of cyanobacteria, this book provides a broad compilation of research that deals with cyanobacterial stress responses in both controlled laboratory conditions as well as in their natural environment.


Book Synopsis Stress Biology of Cyanobacteria by : Ashish Kumar Srivastava

Download or read book Stress Biology of Cyanobacteria written by Ashish Kumar Srivastava and published by CRC Press. This book was released on 2013-03-01 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: A significant component of many different ecosystems, cyanobacteria occupy almost every niche of the earth, including fresh and salt waters, rice fields, hot springs, arid deserts, and polar regions. Cyanobacteria, along with algae, produce nearly half the global oxygen, making assessment of their ecophysiologies important for understanding climate impacts and potential remediation. Stress Biology of Cyanobacteria: Molecular Mechanisms to Cellular Responses is a compilation of holistic responses of cyanobacteria, ranging from ecological and physiological to the modern aspects of their molecular biology, genomics, and biochemistry. Covering almost every aspect of cyanobacterial stress biology, this book is divided into two parts: Bioenergetics and Molecular Mechanisms of Stress Tolerance and Cellular Responses and Ecophysiology. The first few chapters focus on the molecular bioenergetics of photosynthesis and respiration in cyanobacteria, and provide a clear perspective on different stress tolerance mechanisms. Part I also covers the effect of specific stresses—including heavy metal, high and low temperature, salt, osmotic, and UV-B stress—on a wide range of vital physiological, biochemical, and molecular processes of cyanobacteria. Part II describes mechanisms of symbiosis, stress-induced bioproducts, and the role of environmental factors on nitrogen fixation, which along with photosynthesis is a major contributor to the current geochemical status of the planet. The text also covers mutation and cyanobacterial adaptation, and the most widely studied cyanotoxin, microcystin, which has effects on both human and animal health. With contributions from experts around the world, representing the global importance of cyanobacteria, this book provides a broad compilation of research that deals with cyanobacterial stress responses in both controlled laboratory conditions as well as in their natural environment.

Ecology of Cyanobacteria II

Ecology of Cyanobacteria II

Author: Brian A. Whitton

Publisher: Springer Science & Business Media

Published: 2012-07-05

Total Pages: 753

ISBN-13: 9400738552

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Cyanobacteria have existed for 3.5 billion years, yet they are still the most important photosynthetic organisms on the planet for cycling carbon and nitrogen. The ecosystems where they have key roles range from the warmer oceans to many Antarctic sites. They also include dense nuisance growths in nutrient-rich lakes and nitrogen-fixers which aid the fertility of rice-fields and many soils, especially the biological soil crusts of arid regions. Molecular biology has in recent years provided major advances in our understanding of cyanobacterial ecology. Perhaps for more than any other group of organisms, it is possible to see how the ecology, physiology, biochemistry, ultrastructure and molecular biology interact. This all helps to deal with practical problems such as the control of nuisance blooms and the use of cyanobacterial inocula to manage semi-desert soils. Large-scale culture of several organisms, especially "Spirulina" (Arthrospira), for health food and specialist products is increasingly being expanded for a much wider range of uses. In view of their probable contribution to past oil deposits, much attention is currently focused on their potential as a source of biofuel. Please visit http://extras.springer.com/ to view Extra Materials belonging to this volume. This book complements the highly successful Ecology of Cyanobacteria and integrates the discoveries of the past twelve years with the older literature.


Book Synopsis Ecology of Cyanobacteria II by : Brian A. Whitton

Download or read book Ecology of Cyanobacteria II written by Brian A. Whitton and published by Springer Science & Business Media. This book was released on 2012-07-05 with total page 753 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanobacteria have existed for 3.5 billion years, yet they are still the most important photosynthetic organisms on the planet for cycling carbon and nitrogen. The ecosystems where they have key roles range from the warmer oceans to many Antarctic sites. They also include dense nuisance growths in nutrient-rich lakes and nitrogen-fixers which aid the fertility of rice-fields and many soils, especially the biological soil crusts of arid regions. Molecular biology has in recent years provided major advances in our understanding of cyanobacterial ecology. Perhaps for more than any other group of organisms, it is possible to see how the ecology, physiology, biochemistry, ultrastructure and molecular biology interact. This all helps to deal with practical problems such as the control of nuisance blooms and the use of cyanobacterial inocula to manage semi-desert soils. Large-scale culture of several organisms, especially "Spirulina" (Arthrospira), for health food and specialist products is increasingly being expanded for a much wider range of uses. In view of their probable contribution to past oil deposits, much attention is currently focused on their potential as a source of biofuel. Please visit http://extras.springer.com/ to view Extra Materials belonging to this volume. This book complements the highly successful Ecology of Cyanobacteria and integrates the discoveries of the past twelve years with the older literature.

The Molecular Biology of Cyanobacteria

The Molecular Biology of Cyanobacteria

Author: D.A. Bryant

Publisher: Springer Science & Business Media

Published: 2006-05-31

Total Pages: 889

ISBN-13: 9401102279

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More than twenty years ago, as a fledgling graduate some peculiar aspects of the genetics of these student who was just starting to learn about these organisms but to pay respects to the two volumes of organisms that would become my primary research Carr of Whitton that played important roles in my focus, the publication of Noel Carr and Brian own thinking about cyanobacteria (and no doubt in Whitton's The Biology of the Blue-Green Algae in the development of many others as well). Contri 1973 was an event of great significance. Until the buting authors were asked to describe not only what appearance of this treatise, there was no single volume we know at present, but also to point out things we available that presented a broad overview of the don't know yet. I have attempted to assemble a book biology and biochemistry ofthese organisms. Nearly that would stimulate graduate students and other ten years later, I was privileged to be a contributing researchers in the same way that I was affected by the author to Carr and Whitton's sequel volume The books mentioned above. Biology of the Cyanobacteria. Although the It appears that cyanobacterial molecular biologists intervening period had been marked by heated debates have indeed paid attention to the admonition of their over the taxonomy and taxonomic position of the erstwhile colleague, W Ford Doolittle, to 'study organisms, it was also a time when the comparative those things that cyanobacteria do well.


Book Synopsis The Molecular Biology of Cyanobacteria by : D.A. Bryant

Download or read book The Molecular Biology of Cyanobacteria written by D.A. Bryant and published by Springer Science & Business Media. This book was released on 2006-05-31 with total page 889 pages. Available in PDF, EPUB and Kindle. Book excerpt: More than twenty years ago, as a fledgling graduate some peculiar aspects of the genetics of these student who was just starting to learn about these organisms but to pay respects to the two volumes of organisms that would become my primary research Carr of Whitton that played important roles in my focus, the publication of Noel Carr and Brian own thinking about cyanobacteria (and no doubt in Whitton's The Biology of the Blue-Green Algae in the development of many others as well). Contri 1973 was an event of great significance. Until the buting authors were asked to describe not only what appearance of this treatise, there was no single volume we know at present, but also to point out things we available that presented a broad overview of the don't know yet. I have attempted to assemble a book biology and biochemistry ofthese organisms. Nearly that would stimulate graduate students and other ten years later, I was privileged to be a contributing researchers in the same way that I was affected by the author to Carr and Whitton's sequel volume The books mentioned above. Biology of the Cyanobacteria. Although the It appears that cyanobacterial molecular biologists intervening period had been marked by heated debates have indeed paid attention to the admonition of their over the taxonomy and taxonomic position of the erstwhile colleague, W Ford Doolittle, to 'study organisms, it was also a time when the comparative those things that cyanobacteria do well.

Cyanobacteria

Cyanobacteria

Author: A.K. Mishra

Publisher: Academic Press

Published: 2018-11-30

Total Pages: 541

ISBN-13: 0128146680

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Cyanobacteria constitute the most widely distributed group of photosynthetic prokaryotes found in almost all realms of the earth and play an important role in Earth’s nitrogen and carbon cycle. The gradual transformation from reducing atmosphere to oxidizing atmosphere was a turning point in the evolutionary history of the earth and made conditions for present life forms possible. Cyanobacteria: From Basic Science to Applications is the first reference volume that comprehensively discusses all aspects of cyanobacteria, including the diverse mechanisms of cyanobacteria for the advancement of cyanobacterial abilities, towards higher biofuel productivity, enhanced tolerance to environmental stress and bioactive compounds and potential for biofertilizers. Describes cyanobacterial diversity, stress biology, and biotechnological aspects of cyanobacteria Explores the global importance of cyanobacteria Provides a broad compilation of research that deals with cyanobacterial stress responses in both controlled laboratory conditions as well as in their natural environment


Book Synopsis Cyanobacteria by : A.K. Mishra

Download or read book Cyanobacteria written by A.K. Mishra and published by Academic Press. This book was released on 2018-11-30 with total page 541 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanobacteria constitute the most widely distributed group of photosynthetic prokaryotes found in almost all realms of the earth and play an important role in Earth’s nitrogen and carbon cycle. The gradual transformation from reducing atmosphere to oxidizing atmosphere was a turning point in the evolutionary history of the earth and made conditions for present life forms possible. Cyanobacteria: From Basic Science to Applications is the first reference volume that comprehensively discusses all aspects of cyanobacteria, including the diverse mechanisms of cyanobacteria for the advancement of cyanobacterial abilities, towards higher biofuel productivity, enhanced tolerance to environmental stress and bioactive compounds and potential for biofertilizers. Describes cyanobacterial diversity, stress biology, and biotechnological aspects of cyanobacteria Explores the global importance of cyanobacteria Provides a broad compilation of research that deals with cyanobacterial stress responses in both controlled laboratory conditions as well as in their natural environment

Bioenergetic Processes of Cyanobacteria

Bioenergetic Processes of Cyanobacteria

Author: Guenter A. Peschek

Publisher: Springer Science & Business Media

Published: 2011-05-26

Total Pages: 733

ISBN-13: 9400703880

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This publication is unique among a number of books on cyanobacteria because it focuses on the bioenergetics of these widespread organisms which are the evolutionary prerequisite for the development of all higher forms of life on our "blue" planet. The book primarily addresses questions of energy conversion by the fundamental bioenergetic processes: (oxygenic) photosynthesis, (aerobic) respiration, and (anaerobic) fermentation which uniquely occur together in these prokaryotic cells. Thermophilic cyanobacteria offer the most suitable material for high resolution structure analyses of Photosystem I and II and other electron transport complexes by X-ray crystallography (for example, at present the structure of Photosystem II at atomic resolution is only known for these organisms). These achievements during the last decade represent a milestone in our understanding of the complexes which are crucial for solar energy exploitation through photosynthetic water splitting. The present work represents an ambitious attempt to achieve the goal of a synoptic state-of-the-art picture by casting together the mosaics of detailed knowledge described by leading experts in the field. It contains 24 chapters written by 35 authors from Europe, USA, India and Japan. The book is aimed at reaching a broad audience ranging from students to experienced scientists. The editors wish all readers a pleasant and stimulating journey through the fascinating “world” of the bioenergetics of cyanobacteria and sincerely hope that this book will not only be of great value for the experts but also entice young people into this exciting research area with the aim to address successfully the challenging problems of high relevance that are still waiting for a satisfactory answer.


Book Synopsis Bioenergetic Processes of Cyanobacteria by : Guenter A. Peschek

Download or read book Bioenergetic Processes of Cyanobacteria written by Guenter A. Peschek and published by Springer Science & Business Media. This book was released on 2011-05-26 with total page 733 pages. Available in PDF, EPUB and Kindle. Book excerpt: This publication is unique among a number of books on cyanobacteria because it focuses on the bioenergetics of these widespread organisms which are the evolutionary prerequisite for the development of all higher forms of life on our "blue" planet. The book primarily addresses questions of energy conversion by the fundamental bioenergetic processes: (oxygenic) photosynthesis, (aerobic) respiration, and (anaerobic) fermentation which uniquely occur together in these prokaryotic cells. Thermophilic cyanobacteria offer the most suitable material for high resolution structure analyses of Photosystem I and II and other electron transport complexes by X-ray crystallography (for example, at present the structure of Photosystem II at atomic resolution is only known for these organisms). These achievements during the last decade represent a milestone in our understanding of the complexes which are crucial for solar energy exploitation through photosynthetic water splitting. The present work represents an ambitious attempt to achieve the goal of a synoptic state-of-the-art picture by casting together the mosaics of detailed knowledge described by leading experts in the field. It contains 24 chapters written by 35 authors from Europe, USA, India and Japan. The book is aimed at reaching a broad audience ranging from students to experienced scientists. The editors wish all readers a pleasant and stimulating journey through the fascinating “world” of the bioenergetics of cyanobacteria and sincerely hope that this book will not only be of great value for the experts but also entice young people into this exciting research area with the aim to address successfully the challenging problems of high relevance that are still waiting for a satisfactory answer.

CRC Handbook of Symbiotic Cyanobacteria

CRC Handbook of Symbiotic Cyanobacteria

Author: Amar Nath Rai

Publisher: CRC Press

Published: 2018-05-04

Total Pages: 259

ISBN-13: 1351079638

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In one convenient source, this ready reference brings together for the first time, all the information available on various cyanobacterial symbioses/symbiotic cyanobacteria. Comprehensive data on structure, physiology, biochemistry and molecular biology of the cyanobiont in various cyanobacterial symbioses is included. Aplied aspects such as use of Azolla in rice cultivation and artificial symbioses are addressed, along with a chapter dedicated to methodology. This informative new text is useful to researchers, teachers, and students.


Book Synopsis CRC Handbook of Symbiotic Cyanobacteria by : Amar Nath Rai

Download or read book CRC Handbook of Symbiotic Cyanobacteria written by Amar Nath Rai and published by CRC Press. This book was released on 2018-05-04 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: In one convenient source, this ready reference brings together for the first time, all the information available on various cyanobacterial symbioses/symbiotic cyanobacteria. Comprehensive data on structure, physiology, biochemistry and molecular biology of the cyanobiont in various cyanobacterial symbioses is included. Aplied aspects such as use of Azolla in rice cultivation and artificial symbioses are addressed, along with a chapter dedicated to methodology. This informative new text is useful to researchers, teachers, and students.

Handbook on Cyanobacteria

Handbook on Cyanobacteria

Author: Percy M. Gault

Publisher:

Published: 2009

Total Pages: 0

ISBN-13: 9781607410928

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Cyanobacteria, also known as blue-green algae, blue-green bacteria or cyanophyta, is a phylum of bacteria that obtain their energy through photosynthesis. They are a significant component of the marine nitrogen cycle and an important primary producer in many areas of the ocean, but are also found in habitats other than the marine environment; in particular, cyanobacteria are known to occur in both freshwater and hypersaline inland lakes. They are found in almost every conceivable environment, from oceans to fresh water to bare rock to soil. Cyanobacteria are the only group of organisms that are able to reduce nitrogen and carbon in aerobic conditions, a fact that may be responsible for their evolutionary and ecological success. Certain cyanobacteria also produce cyanotoxins. This new book presents a broad variety of international research on this important organism.


Book Synopsis Handbook on Cyanobacteria by : Percy M. Gault

Download or read book Handbook on Cyanobacteria written by Percy M. Gault and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanobacteria, also known as blue-green algae, blue-green bacteria or cyanophyta, is a phylum of bacteria that obtain their energy through photosynthesis. They are a significant component of the marine nitrogen cycle and an important primary producer in many areas of the ocean, but are also found in habitats other than the marine environment; in particular, cyanobacteria are known to occur in both freshwater and hypersaline inland lakes. They are found in almost every conceivable environment, from oceans to fresh water to bare rock to soil. Cyanobacteria are the only group of organisms that are able to reduce nitrogen and carbon in aerobic conditions, a fact that may be responsible for their evolutionary and ecological success. Certain cyanobacteria also produce cyanotoxins. This new book presents a broad variety of international research on this important organism.

Protocols in Lichenology

Protocols in Lichenology

Author: Ilse Kranner

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 586

ISBN-13: 3642563597

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As an intricate association between a fungus and one or more green algae or cyanobacteria, lichens are one of the most successful examples of symbiosis. These fascinating organisms survive extreme desiccation and temperatures. They are adapted to a great variety of habitats, from deserts to intertidal zones, from tropical rain forests to the peaks of the Himalayas and to circumpolar ecosystems. Lichens are extremely efficient accumulators of atmospherically deposited pollutants, and are therefore widely used to monitor environmental pollution. Their wide range of secondary products show pharmaceutically interesting fungicidal, antibacterial and antiviral properties. Lichens are extremely difficult to culture. This manual provides well-tested tissue culture protocols, protocols for studying lichen ultrastructure, (eco)physiology, primary and secondary compounds, and for using lichens as bioindicators.


Book Synopsis Protocols in Lichenology by : Ilse Kranner

Download or read book Protocols in Lichenology written by Ilse Kranner and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 586 pages. Available in PDF, EPUB and Kindle. Book excerpt: As an intricate association between a fungus and one or more green algae or cyanobacteria, lichens are one of the most successful examples of symbiosis. These fascinating organisms survive extreme desiccation and temperatures. They are adapted to a great variety of habitats, from deserts to intertidal zones, from tropical rain forests to the peaks of the Himalayas and to circumpolar ecosystems. Lichens are extremely efficient accumulators of atmospherically deposited pollutants, and are therefore widely used to monitor environmental pollution. Their wide range of secondary products show pharmaceutically interesting fungicidal, antibacterial and antiviral properties. Lichens are extremely difficult to culture. This manual provides well-tested tissue culture protocols, protocols for studying lichen ultrastructure, (eco)physiology, primary and secondary compounds, and for using lichens as bioindicators.