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Book Synopsis Enzyme or Whole Cell Immobilization for Efficient Biocatalysis: Focusing on Novel Supporting Platforms and Immobilization Techniques by : Wen-Yong Lou
Download or read book Enzyme or Whole Cell Immobilization for Efficient Biocatalysis: Focusing on Novel Supporting Platforms and Immobilization Techniques written by Wen-Yong Lou and published by Frontiers Media SA. This book was released on 2021-04-21 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Lignin Chemistry A thorough reference guide to Lignin Chemistry, from inherent structure revealing to transformation into chemicals, fuels, and materials Climate change, driven by rising greenhouse gas emissions, is the defining challenge of our time. Reducing our dependence on non-renewable resources such as fossil fuels will require alternative, more sustainable resources. Lignin, the only widely-occurring, renewable, aromatic bio-polymer in Nature, has a range of application potential in the production of chemicals, fuels, and other industrial materials. Lignin science has become one of the fastest-growing and most significant areas of sustainable chemistry in the world. Lignin Chemistry: Characterization, Isolation, and Valorization presents a systematic, multidisciplinary overview of this cutting-edge field and its current state of research. Beginning with a robust characterization of lignin, the book addresses the isolation and transformation of lignin, as well as the book inspires with a plethora of applications. The result is a critical resource for researchers and professionals in any area of academic or industry where renewable biomass, in particular lignin, has importance. Lignin Chemistry readers will find: Thermochemical and catalytic strategies for lignin conversion Detailed discussion of the valorization of lignin towards biopolymers, nanoparticles, carbon fibers and materials, and hydrogels An authorial team with immense and varied research experience Lignin Chemistry is ideal for chemical engineers, catalytic chemists, biochemists, material scientists, and analytical chemists in industry.
Book Synopsis Lignin Chemistry by : Yuhe Liao
Download or read book Lignin Chemistry written by Yuhe Liao and published by John Wiley & Sons. This book was released on 2024-09-03 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lignin Chemistry A thorough reference guide to Lignin Chemistry, from inherent structure revealing to transformation into chemicals, fuels, and materials Climate change, driven by rising greenhouse gas emissions, is the defining challenge of our time. Reducing our dependence on non-renewable resources such as fossil fuels will require alternative, more sustainable resources. Lignin, the only widely-occurring, renewable, aromatic bio-polymer in Nature, has a range of application potential in the production of chemicals, fuels, and other industrial materials. Lignin science has become one of the fastest-growing and most significant areas of sustainable chemistry in the world. Lignin Chemistry: Characterization, Isolation, and Valorization presents a systematic, multidisciplinary overview of this cutting-edge field and its current state of research. Beginning with a robust characterization of lignin, the book addresses the isolation and transformation of lignin, as well as the book inspires with a plethora of applications. The result is a critical resource for researchers and professionals in any area of academic or industry where renewable biomass, in particular lignin, has importance. Lignin Chemistry readers will find: Thermochemical and catalytic strategies for lignin conversion Detailed discussion of the valorization of lignin towards biopolymers, nanoparticles, carbon fibers and materials, and hydrogels An authorial team with immense and varied research experience Lignin Chemistry is ideal for chemical engineers, catalytic chemists, biochemists, material scientists, and analytical chemists in industry.
Nanomaterials for Biocatalysis explains the fundamental design concepts and emerging applications of nanoscale biocatalysts, such as bioconversions, bioelectronics, biosensors, biocomputing and therapeutic applications. Nano-biocatalysts refers to the incorporation of enzymes into nanomaterials. These enzyme-enhanced nanocarriers have many advantages, including low mass transfer limitation, high enzyme capacity, better stabilization, and the formation of single-enzyme nanoparticles. Smart nanocontainers have been developed for the smart release of their embedded active substances. These smart releases can be obtained by using smart coatings as their outer nanoshells. In addition, these nanocontainers could protect the enzymes from chemical or metabolic alterations on their delivering pathways towards the target. This is an important reference source for materials scientists and chemical engineers who want to know more about how nanomaterials are being used for biocatalysis applications. Explains the major fabrication techniques and applications of nanobiocatalysts Shows how nanobiocatalysts are used in a variety of environmental and biomedical sectors Assesses the major challenges associated with the widespread manufacture of nanobiocatalysts
Book Synopsis Nanomaterials for Biocatalysis by : Guillermo R. Castro
Download or read book Nanomaterials for Biocatalysis written by Guillermo R. Castro and published by Elsevier. This book was released on 2021-10-12 with total page 755 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomaterials for Biocatalysis explains the fundamental design concepts and emerging applications of nanoscale biocatalysts, such as bioconversions, bioelectronics, biosensors, biocomputing and therapeutic applications. Nano-biocatalysts refers to the incorporation of enzymes into nanomaterials. These enzyme-enhanced nanocarriers have many advantages, including low mass transfer limitation, high enzyme capacity, better stabilization, and the formation of single-enzyme nanoparticles. Smart nanocontainers have been developed for the smart release of their embedded active substances. These smart releases can be obtained by using smart coatings as their outer nanoshells. In addition, these nanocontainers could protect the enzymes from chemical or metabolic alterations on their delivering pathways towards the target. This is an important reference source for materials scientists and chemical engineers who want to know more about how nanomaterials are being used for biocatalysis applications. Explains the major fabrication techniques and applications of nanobiocatalysts Shows how nanobiocatalysts are used in a variety of environmental and biomedical sectors Assesses the major challenges associated with the widespread manufacture of nanobiocatalysts
This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.
Book Synopsis Biocatalysis for Practitioners by : Gonzalo de Gonzalo
Download or read book Biocatalysis for Practitioners written by Gonzalo de Gonzalo and published by John Wiley & Sons. This book was released on 2021-04-13 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.
The concept of a circular economy relies on waste reduction, valorization, and recycling. Global trends for “green” synthesis of chemicals have positioned the field of enzyme technology and biocatalysis (multi-enzymes and whole-cells) as an alternative for the synthesis of more social- and environmentally-responsible bio-based chemicals. Recent advances in synthetic biology, computational tools, and metabolic engineering have supported the discovery of new enzymes and the rational design of whole-cell biocatalysts. In this book, we highlight these current advances in the field of biocatalysis, with special emphasis on novel enzymes and whole-cell biocatalysts for applications in several industrial biotechnological applications.
Book Synopsis Novel Enzyme and Whole-Cell Biocatalysts by : Anwar Sunna
Download or read book Novel Enzyme and Whole-Cell Biocatalysts written by Anwar Sunna and published by MDPI. This book was released on 2020-11-04 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: The concept of a circular economy relies on waste reduction, valorization, and recycling. Global trends for “green” synthesis of chemicals have positioned the field of enzyme technology and biocatalysis (multi-enzymes and whole-cells) as an alternative for the synthesis of more social- and environmentally-responsible bio-based chemicals. Recent advances in synthetic biology, computational tools, and metabolic engineering have supported the discovery of new enzymes and the rational design of whole-cell biocatalysts. In this book, we highlight these current advances in the field of biocatalysis, with special emphasis on novel enzymes and whole-cell biocatalysts for applications in several industrial biotechnological applications.
This book reviews the development of cell-free production platforms and offers an authoritative perspective of the latest advances and methodologies in cell-free production systems. Readers will discover the biomanufacturing potential of in vitro biotransformation (ivBT) employing purified cascade multi-enzymes, the development of hydrogel-based multi-enzymatic systems for biosynthesis, and novel insights into the optimization of biocatalytic processes. Additionally, the book explores the cell-free production and regeneration of cofactors, shedding light on strategies to enhance the efficiency and sustainability of cellular processes. In this book, particular attention is given to the progress of cell-free in vitro evolution techniques for optimizing enzyme performance, and the book also presents the integration of rapid and finely-tuned expression systems for deployable sensing applications, revolutionizing the field of biosensing. The synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes are explored, providing a deep understanding of cellular function at the molecular level. Lastly, the book covers compartmentalized cell-free expression systems for building synthetic cells, showcasing the potential for constructing artificial cellular systems with unique functionalities. Given its breadth, this book appeals to academics, researchers, and professionals interested in the forefront of biotechnology, and together with the companion volume “Cell-free Macromolecular Synthesis”, both books highlight the research progresses on the basic and applied research of cell-free production systems in the last few years, being invaluable resources in the field. Chapter “Cell-free synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Book Synopsis Cell-free Production by : Yuan Lu
Download or read book Cell-free Production written by Yuan Lu and published by Springer Nature. This book was released on 2023-10-18 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the development of cell-free production platforms and offers an authoritative perspective of the latest advances and methodologies in cell-free production systems. Readers will discover the biomanufacturing potential of in vitro biotransformation (ivBT) employing purified cascade multi-enzymes, the development of hydrogel-based multi-enzymatic systems for biosynthesis, and novel insights into the optimization of biocatalytic processes. Additionally, the book explores the cell-free production and regeneration of cofactors, shedding light on strategies to enhance the efficiency and sustainability of cellular processes. In this book, particular attention is given to the progress of cell-free in vitro evolution techniques for optimizing enzyme performance, and the book also presents the integration of rapid and finely-tuned expression systems for deployable sensing applications, revolutionizing the field of biosensing. The synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes are explored, providing a deep understanding of cellular function at the molecular level. Lastly, the book covers compartmentalized cell-free expression systems for building synthetic cells, showcasing the potential for constructing artificial cellular systems with unique functionalities. Given its breadth, this book appeals to academics, researchers, and professionals interested in the forefront of biotechnology, and together with the companion volume “Cell-free Macromolecular Synthesis”, both books highlight the research progresses on the basic and applied research of cell-free production systems in the last few years, being invaluable resources in the field. Chapter “Cell-free synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
With more than 40 contributions from expert authors, this is an extensive overview of all important research topics in the field of bioengineering, including metabolic engineering, biotransformations and biomedical applications. Alongside several chapters dealing with biotransformations and biocatalysis, a whole section is devoted to biofuels and the utilization of biomass. Current perspectives on synthetic biology and metabolic engineering approaches are presented, involving such example organisms as Escherichia coli and Corynebacterium glutamicum, while a further section covers topics in biomedical engineering including drug delivery systems and biopharmaceuticals. The book concludes with chapters on computer-aided bioprocess engineering and systems biology. This is a part of the Advanced Biotechnology book series, covering all pertinent aspects of the field with each volume prepared by eminent scientists who are experts on the topic in question. Invaluable reading for biotechnologists and bioengineers, as well as those working in the chemical and pharmaceutical industries. Advanced Biotechnology Biotechnology is a broad, interdisciplinary field of science, combining biological sciences and relevant engineering disciplines, that is becoming increasingly important as it benefits the environment and society as a whole. Recent years have seen substantial advances in all areas of biotechnology, resulting in the emergence of brand new fields. To reflect this progress, Sang-Yup Lee (KAIST, South Korea), Jens Nielsen (Chalmers University, Sweden), and Gregory Stephanopoulos (MIT, USA) have joined forces as the editors of a new Wiley-VCH book series. Advanced Biotechnology will cover all pertinent aspects of the field and each volume will be prepared by eminent scientists who are experts on the topic in question.
Book Synopsis Emerging Areas in Bioengineering by : Ho Nam Chang
Download or read book Emerging Areas in Bioengineering written by Ho Nam Chang and published by John Wiley & Sons. This book was released on 2018-03-12 with total page 898 pages. Available in PDF, EPUB and Kindle. Book excerpt: With more than 40 contributions from expert authors, this is an extensive overview of all important research topics in the field of bioengineering, including metabolic engineering, biotransformations and biomedical applications. Alongside several chapters dealing with biotransformations and biocatalysis, a whole section is devoted to biofuels and the utilization of biomass. Current perspectives on synthetic biology and metabolic engineering approaches are presented, involving such example organisms as Escherichia coli and Corynebacterium glutamicum, while a further section covers topics in biomedical engineering including drug delivery systems and biopharmaceuticals. The book concludes with chapters on computer-aided bioprocess engineering and systems biology. This is a part of the Advanced Biotechnology book series, covering all pertinent aspects of the field with each volume prepared by eminent scientists who are experts on the topic in question. Invaluable reading for biotechnologists and bioengineers, as well as those working in the chemical and pharmaceutical industries. Advanced Biotechnology Biotechnology is a broad, interdisciplinary field of science, combining biological sciences and relevant engineering disciplines, that is becoming increasingly important as it benefits the environment and society as a whole. Recent years have seen substantial advances in all areas of biotechnology, resulting in the emergence of brand new fields. To reflect this progress, Sang-Yup Lee (KAIST, South Korea), Jens Nielsen (Chalmers University, Sweden), and Gregory Stephanopoulos (MIT, USA) have joined forces as the editors of a new Wiley-VCH book series. Advanced Biotechnology will cover all pertinent aspects of the field and each volume will be prepared by eminent scientists who are experts on the topic in question.
Book Synopsis Microorganism and process engineering for biosynthesis by : Qi Xianghui
Download or read book Microorganism and process engineering for biosynthesis written by Qi Xianghui and published by Frontiers Media SA. This book was released on 2023-07-27 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt:
This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.
Book Synopsis Biocatalysis for Practitioners by : Gonzalo de Gonzalo
Download or read book Biocatalysis for Practitioners written by Gonzalo de Gonzalo and published by John Wiley & Sons. This book was released on 2021-07-19 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.
Free enzymes are promising catalytic agents because their activities can be achieved in the absence of growth substrates and conditions required for live microorganisms. Immobilizing enzymes on solid supports have been shown to improve their stability and longevity. To date, numerous enzyme immobilization supports and techniques have been developed, however, several associated issues, such as reduced enzymatic activity, low immobilization efficiency, and involvement of toxic substances, still limit the application of enzymes, particularly in environmental remediation. In this research, a novel enzyme immobilization approach was developed and evaluated for removing environmental contaminants. The vault particles, members of protein nanocages, are entirely biocompatible and biodegradable, and have large empty cores for anchoring enzyme molecules, thus serving as eco-friendly enzyme carriers in environmental applications. Manganese peroxidase (MnP), which has been demonstrated to be efficient in biodegrading various environmental contaminants, was employed as a model enzyme in the present study. MnP was encapsulated into vault nanoparticles through the previously developed INT strategy, and the resultant vault-encapsulated MnP showed improved thermal stability and wider pH adaptability than free enzymes. The encapsulated MnP exhibited significantly better biodegradation of phenol than that catalyzed by native MnP. The performance of vault-encapsulated MnP was subsequently evaluated for removing and detoxifying three bisphenolic compounds, BPA, BPF, BPAP, which are trace contaminants of concern in water supplies. Compared with free MnP enzymes, encapsulated MnP showed longer durability in reactions, high removal rates and efficiency, and different product profiles. Further reproductive toxicity studies in Caenorhabditis elegans demonstrated that products resulted from catalysis by vault-encapsulated MnP induced less germline apoptosis and led to lower fertility damage. Synthesis and proper assembly of vault particles in yeast Pichia pastoris cells were demonstrated for the first time in this study. The yeast production system showed comparable vault yield to the currently used insect cell system, but at more than ten times lower cost, which makes production of large quantities of vault particles for industrial, commercial, and environmental applications possible. A vault-templating route to porous silica nanoparticles was developed. Combining it with vault encapsulation, a new enzyme immobilization approach with high immobilization efficiency and enzyme activity yield, and low leakage was demonstrated. MnP immobilized in vault/silica was more stable than free MnP as well as vault-encapsulated MnP, and accomplished high activity and excellent reusability. Taken together, this research demonstrates the feasibility of using vault particles as enzyme carriers for water treatment and environmental applications. This work serves as a foundation for using customized vaults packaged with biodegradative enzymes to target specific contaminant groups in various industrial and environmental applications.
Book Synopsis Vault Nanoparticles-Based Enzyme Immobilization Platform for Environmental Applications by : Meng Wang
Download or read book Vault Nanoparticles-Based Enzyme Immobilization Platform for Environmental Applications written by Meng Wang and published by . This book was released on 2018 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: Free enzymes are promising catalytic agents because their activities can be achieved in the absence of growth substrates and conditions required for live microorganisms. Immobilizing enzymes on solid supports have been shown to improve their stability and longevity. To date, numerous enzyme immobilization supports and techniques have been developed, however, several associated issues, such as reduced enzymatic activity, low immobilization efficiency, and involvement of toxic substances, still limit the application of enzymes, particularly in environmental remediation. In this research, a novel enzyme immobilization approach was developed and evaluated for removing environmental contaminants. The vault particles, members of protein nanocages, are entirely biocompatible and biodegradable, and have large empty cores for anchoring enzyme molecules, thus serving as eco-friendly enzyme carriers in environmental applications. Manganese peroxidase (MnP), which has been demonstrated to be efficient in biodegrading various environmental contaminants, was employed as a model enzyme in the present study. MnP was encapsulated into vault nanoparticles through the previously developed INT strategy, and the resultant vault-encapsulated MnP showed improved thermal stability and wider pH adaptability than free enzymes. The encapsulated MnP exhibited significantly better biodegradation of phenol than that catalyzed by native MnP. The performance of vault-encapsulated MnP was subsequently evaluated for removing and detoxifying three bisphenolic compounds, BPA, BPF, BPAP, which are trace contaminants of concern in water supplies. Compared with free MnP enzymes, encapsulated MnP showed longer durability in reactions, high removal rates and efficiency, and different product profiles. Further reproductive toxicity studies in Caenorhabditis elegans demonstrated that products resulted from catalysis by vault-encapsulated MnP induced less germline apoptosis and led to lower fertility damage. Synthesis and proper assembly of vault particles in yeast Pichia pastoris cells were demonstrated for the first time in this study. The yeast production system showed comparable vault yield to the currently used insect cell system, but at more than ten times lower cost, which makes production of large quantities of vault particles for industrial, commercial, and environmental applications possible. A vault-templating route to porous silica nanoparticles was developed. Combining it with vault encapsulation, a new enzyme immobilization approach with high immobilization efficiency and enzyme activity yield, and low leakage was demonstrated. MnP immobilized in vault/silica was more stable than free MnP as well as vault-encapsulated MnP, and accomplished high activity and excellent reusability. Taken together, this research demonstrates the feasibility of using vault particles as enzyme carriers for water treatment and environmental applications. This work serves as a foundation for using customized vaults packaged with biodegradative enzymes to target specific contaminant groups in various industrial and environmental applications.
