Atlas of Human Pluripotent Stem Cells in Culture

Atlas of Human Pluripotent Stem Cells in Culture

Author: Lyn Healy

Publisher: Springer

Published: 2014-11-07

Total Pages: 216

ISBN-13: 1489975071

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This lavishly-illustrated, authoritative atlas explores the intricate art of culturing human pluripotent stem cells. Twelve chapters – containing more than 280 color illustrations – cover a variety of topics in pluripotent stem cell culturing including mouse and human fibroblasts, human embryonic stem cells and induced pluripotent stem cells, characteristic staining patterns, and abnormal cultures, among others. Atlas of Human Pluripotent Stem Cells in Culture is a comprehensive collection of illustrated techniques complemented by informative and educational captions examining what good quality cells look like and how they behave in various environments. Examples of perfect cultures are compared side-by-side to less-than-perfect and unacceptable examples of human embryonic and induced pluripotent stem cell colonies. This detailed and thorough atlas is an invaluable resource for researchers, teachers, and students who are interested in or working with stem cell culturing.


Book Synopsis Atlas of Human Pluripotent Stem Cells in Culture by : Lyn Healy

Download or read book Atlas of Human Pluripotent Stem Cells in Culture written by Lyn Healy and published by Springer. This book was released on 2014-11-07 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: This lavishly-illustrated, authoritative atlas explores the intricate art of culturing human pluripotent stem cells. Twelve chapters – containing more than 280 color illustrations – cover a variety of topics in pluripotent stem cell culturing including mouse and human fibroblasts, human embryonic stem cells and induced pluripotent stem cells, characteristic staining patterns, and abnormal cultures, among others. Atlas of Human Pluripotent Stem Cells in Culture is a comprehensive collection of illustrated techniques complemented by informative and educational captions examining what good quality cells look like and how they behave in various environments. Examples of perfect cultures are compared side-by-side to less-than-perfect and unacceptable examples of human embryonic and induced pluripotent stem cell colonies. This detailed and thorough atlas is an invaluable resource for researchers, teachers, and students who are interested in or working with stem cell culturing.

Atlas of Human Pluripotent Stem Cells

Atlas of Human Pluripotent Stem Cells

Author: Michal Amit

Publisher: Springer Science & Business Media

Published: 2011-11-19

Total Pages: 151

ISBN-13: 1617795488

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Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research. The emergence of state of the art culturing techniques is promoting the realization of the full potential of pluripotent stem cells in basic and translational research and in cell-based therapies. This comprehensive and authoritative atlas summarizes more than a decade of experience accumulated by a leading research team in this field. Hands-on step-by-step guidance for the derivation and culturing of human pluripotent stem cells in defined conditions (animal product-free, serum-free, feeder-free) and in non-adhesion suspension culture are provided, as well as methods for examining pluripotency (embryoid body and teratoma formation) and karyotype stability. The Atlas of Human Pluripotent Stem Cells - Derivation and Culturing will serve as a reference and guide to established researchers and those wishing to enter the promising field of pluripotent stem cells.


Book Synopsis Atlas of Human Pluripotent Stem Cells by : Michal Amit

Download or read book Atlas of Human Pluripotent Stem Cells written by Michal Amit and published by Springer Science & Business Media. This book was released on 2011-11-19 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research. The emergence of state of the art culturing techniques is promoting the realization of the full potential of pluripotent stem cells in basic and translational research and in cell-based therapies. This comprehensive and authoritative atlas summarizes more than a decade of experience accumulated by a leading research team in this field. Hands-on step-by-step guidance for the derivation and culturing of human pluripotent stem cells in defined conditions (animal product-free, serum-free, feeder-free) and in non-adhesion suspension culture are provided, as well as methods for examining pluripotency (embryoid body and teratoma formation) and karyotype stability. The Atlas of Human Pluripotent Stem Cells - Derivation and Culturing will serve as a reference and guide to established researchers and those wishing to enter the promising field of pluripotent stem cells.

Human Naïve Pluripotent Stem Cells

Human Naïve Pluripotent Stem Cells

Author: Peter Rugg-Gunn

Publisher: Humana

Published: 2021-12-07

Total Pages: 288

ISBN-13: 9781071619070

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This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.


Book Synopsis Human Naïve Pluripotent Stem Cells by : Peter Rugg-Gunn

Download or read book Human Naïve Pluripotent Stem Cells written by Peter Rugg-Gunn and published by Humana. This book was released on 2021-12-07 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.

Human Pluripotent Stem Cells

Human Pluripotent Stem Cells

Author: Philip H. Schwartz

Publisher: Humana Press

Published: 2011-08-09

Total Pages: 468

ISBN-13: 9781617792007

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Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments. Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.


Book Synopsis Human Pluripotent Stem Cells by : Philip H. Schwartz

Download or read book Human Pluripotent Stem Cells written by Philip H. Schwartz and published by Humana Press. This book was released on 2011-08-09 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments. Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.

Induced Pluripotent Stem (iPS) Cells

Induced Pluripotent Stem (iPS) Cells

Author: Andras Nagy

Publisher: Springer Nature

Published: 2022-06-22

Total Pages: 829

ISBN-13: 107162119X

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This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols, Second Edition aims to arm stem cell biologists, both novice and expert, with invaluable protocols that are currently being used in various laboratories around the world.


Book Synopsis Induced Pluripotent Stem (iPS) Cells by : Andras Nagy

Download or read book Induced Pluripotent Stem (iPS) Cells written by Andras Nagy and published by Springer Nature. This book was released on 2022-06-22 with total page 829 pages. Available in PDF, EPUB and Kindle. Book excerpt: This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols, Second Edition aims to arm stem cell biologists, both novice and expert, with invaluable protocols that are currently being used in various laboratories around the world.

Human Pluripotent Stem Cells

Human Pluripotent Stem Cells

Author: Uma Lakshmipathy

Publisher: John Wiley & Sons

Published: 2018-02-13

Total Pages: 175

ISBN-13: 1119394333

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Comprehensive coverage of the entire induced pluripotent stem cell basic work flow Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential. Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms. Includes helpful tips and tricks that are normally omitted from regular research papers Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations Presents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner Written by experts with extensive experience in stem cell technologies Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.


Book Synopsis Human Pluripotent Stem Cells by : Uma Lakshmipathy

Download or read book Human Pluripotent Stem Cells written by Uma Lakshmipathy and published by John Wiley & Sons. This book was released on 2018-02-13 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive coverage of the entire induced pluripotent stem cell basic work flow Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential. Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms. Includes helpful tips and tricks that are normally omitted from regular research papers Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations Presents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner Written by experts with extensive experience in stem cell technologies Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.

Hematopoietic Differentiation of Human Pluripotent Stem Cells

Hematopoietic Differentiation of Human Pluripotent Stem Cells

Author: Tao Cheng

Publisher: Springer

Published: 2015-08-25

Total Pages: 128

ISBN-13: 9401773122

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This book features the most cutting-edge work from the world’s leading laboratories in this field and provides practical methods for differentiating pluripotent stem cells into hematopoietic lineages in the blood system. Pluripotent stem cells have attracted major interest from a fast-growing and multidisciplinary community of researchers who are developing new techniques for the derivation and differentiation of these cells into specific cell lineages. These direct differentiation methods hold great promise for the translational applications of these cells. This book is an essential reference work for researchers at all levels in the fields of hematology and stem cell biology, as well as clinical practitioners in regenerative medicine.


Book Synopsis Hematopoietic Differentiation of Human Pluripotent Stem Cells by : Tao Cheng

Download or read book Hematopoietic Differentiation of Human Pluripotent Stem Cells written by Tao Cheng and published by Springer. This book was released on 2015-08-25 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book features the most cutting-edge work from the world’s leading laboratories in this field and provides practical methods for differentiating pluripotent stem cells into hematopoietic lineages in the blood system. Pluripotent stem cells have attracted major interest from a fast-growing and multidisciplinary community of researchers who are developing new techniques for the derivation and differentiation of these cells into specific cell lineages. These direct differentiation methods hold great promise for the translational applications of these cells. This book is an essential reference work for researchers at all levels in the fields of hematology and stem cell biology, as well as clinical practitioners in regenerative medicine.

Human Pluripotent Stem Cells

Human Pluripotent Stem Cells

Author:

Publisher: Springer

Published: 2011-08-07

Total Pages: 490

ISBN-13: 9781617792021

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Book Synopsis Human Pluripotent Stem Cells by :

Download or read book Human Pluripotent Stem Cells written by and published by Springer. This book was released on 2011-08-07 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Pluripotent Stem Cells

Pluripotent Stem Cells

Author: Minoru Tomizawa

Publisher: BoD – Books on Demand

Published: 2016-07-20

Total Pages: 534

ISBN-13: 9535124714

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Pluripotent stem cells have distinct characteristics: self-renewal and the potential to differentiate into various somatic cells. In recent years, substantial advances have been made from basic science to clinical applications. The vast amount knowledge available makes obtaining concise yet sufficient information difficult, hence the purpose of this book. In this book, embryonic stem cells, induced pluripotent stem cells, and mesenchymal stem cells are discussed. The book is divided into five sections: pluripotency, culture methods, toxicology, disease models, and regenerative medicine. The topics covered range from new concepts to current technologies. Readers are expected to gain useful information from expert contributors.


Book Synopsis Pluripotent Stem Cells by : Minoru Tomizawa

Download or read book Pluripotent Stem Cells written by Minoru Tomizawa and published by BoD – Books on Demand. This book was released on 2016-07-20 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pluripotent stem cells have distinct characteristics: self-renewal and the potential to differentiate into various somatic cells. In recent years, substantial advances have been made from basic science to clinical applications. The vast amount knowledge available makes obtaining concise yet sufficient information difficult, hence the purpose of this book. In this book, embryonic stem cells, induced pluripotent stem cells, and mesenchymal stem cells are discussed. The book is divided into five sections: pluripotency, culture methods, toxicology, disease models, and regenerative medicine. The topics covered range from new concepts to current technologies. Readers are expected to gain useful information from expert contributors.

Stem Cells and the Future of Regenerative Medicine

Stem Cells and the Future of Regenerative Medicine

Author: Institute of Medicine

Publisher: National Academies Press

Published: 2002-01-25

Total Pages: 112

ISBN-13: 0309170427

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Recent scientific breakthroughs, celebrity patient advocates, and conflicting religious beliefs have come together to bring the state of stem cell researchâ€"specifically embryonic stem cell researchâ€"into the political crosshairs. President Bush's watershed policy statement allows federal funding for embryonic stem cell research but only on a limited number of stem cell lines. Millions of Americans could be affected by the continuing political debate among policymakers and the public. Stem Cells and the Future of Regenerative Medicine provides a deeper exploration of the biological, ethical, and funding questions prompted by the therapeutic potential of undifferentiated human cells. In terms accessible to lay readers, the book summarizes what we know about adult and embryonic stem cells and discusses how to go about the transition from mouse studies to research that has therapeutic implications for people. Perhaps most important, Stem Cells and the Future of Regenerative Medicine also provides an overview of the moral and ethical problems that arise from the use of embryonic stem cells. This timely book compares the impact of public and private research funding and discusses approaches to appropriate research oversight. Based on the insights of leading scientists, ethicists, and other authorities, the book offers authoritative recommendations regarding the use of existing stem cell lines versus new lines in research, the important role of the federal government in this field of research, and other fundamental issues.


Book Synopsis Stem Cells and the Future of Regenerative Medicine by : Institute of Medicine

Download or read book Stem Cells and the Future of Regenerative Medicine written by Institute of Medicine and published by National Academies Press. This book was released on 2002-01-25 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent scientific breakthroughs, celebrity patient advocates, and conflicting religious beliefs have come together to bring the state of stem cell researchâ€"specifically embryonic stem cell researchâ€"into the political crosshairs. President Bush's watershed policy statement allows federal funding for embryonic stem cell research but only on a limited number of stem cell lines. Millions of Americans could be affected by the continuing political debate among policymakers and the public. Stem Cells and the Future of Regenerative Medicine provides a deeper exploration of the biological, ethical, and funding questions prompted by the therapeutic potential of undifferentiated human cells. In terms accessible to lay readers, the book summarizes what we know about adult and embryonic stem cells and discusses how to go about the transition from mouse studies to research that has therapeutic implications for people. Perhaps most important, Stem Cells and the Future of Regenerative Medicine also provides an overview of the moral and ethical problems that arise from the use of embryonic stem cells. This timely book compares the impact of public and private research funding and discusses approaches to appropriate research oversight. Based on the insights of leading scientists, ethicists, and other authorities, the book offers authoritative recommendations regarding the use of existing stem cell lines versus new lines in research, the important role of the federal government in this field of research, and other fundamental issues.