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Book Synopsis Hyperspectral and Filter-based Raman Microscopy and Imaging by : Kenneth Alan Christensen
Download or read book Hyperspectral and Filter-based Raman Microscopy and Imaging written by Kenneth Alan Christensen and published by . This book was released on 1997 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:
This second edition of the successful ready reference is updated and revised with approximately 30% new content to reflect the numerous instrumental developments and improvements, as well as the significant expansion of this rapidly developing field. For example, the combination of IR imaging with AFM has enhanced the achievable lateral resolution by an order of magnitude down to a few hundred nanometers, thus launching a multiplicity of new applications in material science. Furthermore, Raman and IR spectroscopic imaging have become key technologies for the life sciences and today contribute tremendously to a better and more detailed understanding of numerous biological and medical research topics. The topical structure of this new edition is now subdivided into four parts. The first treats the fundamentals of the instrumentation for infrared and Raman imaging and mapping and an overview on the chemometric tools for image analysis. The second part describes a wide varie-ty of applications ranging from biomedical via food, agriculture and plants to polymers and pharmaceuticals. This is followed by a description of imaging techniques operating beyond the diffraction limit, while the final part covers special methodical developments and their utility in specific fields. With its many valuable practical tips, this is a must-have overview for researchers in academic and industrial laboratories wishing to obtain reliable results with this method.
Book Synopsis Infrared and Raman Spectroscopic Imaging by : Reiner Salzer
Download or read book Infrared and Raman Spectroscopic Imaging written by Reiner Salzer and published by John Wiley & Sons. This book was released on 2014-11-03 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition of the successful ready reference is updated and revised with approximately 30% new content to reflect the numerous instrumental developments and improvements, as well as the significant expansion of this rapidly developing field. For example, the combination of IR imaging with AFM has enhanced the achievable lateral resolution by an order of magnitude down to a few hundred nanometers, thus launching a multiplicity of new applications in material science. Furthermore, Raman and IR spectroscopic imaging have become key technologies for the life sciences and today contribute tremendously to a better and more detailed understanding of numerous biological and medical research topics. The topical structure of this new edition is now subdivided into four parts. The first treats the fundamentals of the instrumentation for infrared and Raman imaging and mapping and an overview on the chemometric tools for image analysis. The second part describes a wide varie-ty of applications ranging from biomedical via food, agriculture and plants to polymers and pharmaceuticals. This is followed by a description of imaging techniques operating beyond the diffraction limit, while the final part covers special methodical developments and their utility in specific fields. With its many valuable practical tips, this is a must-have overview for researchers in academic and industrial laboratories wishing to obtain reliable results with this method.
Raman imaging has long been used to probe the chemical nature of a sample, providing information on molecular orientation, symmetry and structure with sub-micron spatial resolution. Recent technical developments have pushed the limits of micro-Raman microscopy, enabling the acquisition of Raman spectra with unprecedented speed, and opening a pathway to fast chemical imaging for many applications from material science and semiconductors to pharmaceutical drug development and cell biology, and even art and forensic science. The promise of tip-enhanced raman spectroscopy (TERS) and near-field techniques is pushing the envelope even further by breaking the limit of diffraction and enabling nano-Raman microscopy.
Book Synopsis Raman Imaging by : Arnaud Zoubir
Download or read book Raman Imaging written by Arnaud Zoubir and published by Springer. This book was released on 2012-07-27 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: Raman imaging has long been used to probe the chemical nature of a sample, providing information on molecular orientation, symmetry and structure with sub-micron spatial resolution. Recent technical developments have pushed the limits of micro-Raman microscopy, enabling the acquisition of Raman spectra with unprecedented speed, and opening a pathway to fast chemical imaging for many applications from material science and semiconductors to pharmaceutical drug development and cell biology, and even art and forensic science. The promise of tip-enhanced raman spectroscopy (TERS) and near-field techniques is pushing the envelope even further by breaking the limit of diffraction and enabling nano-Raman microscopy.
This book includes some very recent applications and the newest emerging trends of hyper-spectral imaging (HSI). HSI is a very recent and strange beast, a sort of a melting pot of previous techniques and scientific interests, merging and concentrating the efforts of physicists, chemists, botanists, biologists, and physicians, to mention just a few, as well as experts in data crunching and statistical elaboration. For almost a century, scientific observation, from looking to planets and stars down to our own cells and below, could be divided into two main categories: analyzing objects on the basis of their physical dimension (recording size, position, weight, etc. and their variations) or on how the object emits, reflects, or absorbs part of the electromagnetic spectrum, i.e., spectroscopy. While the two aspects have been obviously entangled, instruments and skills have always been clearly distinct from each other. With HSI now available, this is no longer the case. This instrument can return specimen dimensionalities and spectroscopic properties to any single pixel of your specimen, in a single set of data. HSI modality is ubiquitous and scale-invariant enough to be used to mark terrestrial resources on the basis of a land map obtained from satellite observation (actually, the oldest application of this type) or to understand if the cell you are looking at is cancerous or perfectly healthy. For all these reasons, HSI represents one of the most exciting methodologies of the new millennium.
Book Synopsis The Future of Hyperspectral Imaging by : Stefano Selci
Download or read book The Future of Hyperspectral Imaging written by Stefano Selci and published by MDPI. This book was released on 2019-11-20 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book includes some very recent applications and the newest emerging trends of hyper-spectral imaging (HSI). HSI is a very recent and strange beast, a sort of a melting pot of previous techniques and scientific interests, merging and concentrating the efforts of physicists, chemists, botanists, biologists, and physicians, to mention just a few, as well as experts in data crunching and statistical elaboration. For almost a century, scientific observation, from looking to planets and stars down to our own cells and below, could be divided into two main categories: analyzing objects on the basis of their physical dimension (recording size, position, weight, etc. and their variations) or on how the object emits, reflects, or absorbs part of the electromagnetic spectrum, i.e., spectroscopy. While the two aspects have been obviously entangled, instruments and skills have always been clearly distinct from each other. With HSI now available, this is no longer the case. This instrument can return specimen dimensionalities and spectroscopic properties to any single pixel of your specimen, in a single set of data. HSI modality is ubiquitous and scale-invariant enough to be used to mark terrestrial resources on the basis of a land map obtained from satellite observation (actually, the oldest application of this type) or to understand if the cell you are looking at is cancerous or perfectly healthy. For all these reasons, HSI represents one of the most exciting methodologies of the new millennium.
The First Book on CRS MicroscopyCompared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Sc
Book Synopsis Coherent Raman Scattering Microscopy by : Ji-Xin Cheng
Download or read book Coherent Raman Scattering Microscopy written by Ji-Xin Cheng and published by CRC Press. This book was released on 2016-04-19 with total page 603 pages. Available in PDF, EPUB and Kindle. Book excerpt: The First Book on CRS MicroscopyCompared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Sc
Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Book Synopsis Stimulated Raman Scattering Microscopy by : Ji-Xin Cheng
Download or read book Stimulated Raman Scattering Microscopy written by Ji-Xin Cheng and published by Elsevier. This book was released on 2021-12-04 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Hyperspectral Imaging, Volume 32, presents a comprehensive exploration of the different analytical methodologies applied on hyperspectral imaging and a state-of-the-art analysis of applications in different scientific and industrial areas. This book presents, for the first time, a comprehensive collection of the main multivariate algorithms used for hyperspectral image analysis in different fields of application. The benefits, drawbacks and suitability of each are fully discussed, along with examples of their application. Users will find state-of-the art information on the machinery for hyperspectral image acquisition, along with a critical assessment of the usage of hyperspectral imaging in diverse scientific fields. Provides a comprehensive roadmap of hyperspectral image analysis, with benefits and considerations for each method discussed Covers state-of-the-art applications in different scientific fields Discusses the implementation of hyperspectral devices in different environments
Book Synopsis Hyperspectral Imaging by :
Download or read book Hyperspectral Imaging written by and published by Elsevier. This book was released on 2019-09-29 with total page 800 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hyperspectral Imaging, Volume 32, presents a comprehensive exploration of the different analytical methodologies applied on hyperspectral imaging and a state-of-the-art analysis of applications in different scientific and industrial areas. This book presents, for the first time, a comprehensive collection of the main multivariate algorithms used for hyperspectral image analysis in different fields of application. The benefits, drawbacks and suitability of each are fully discussed, along with examples of their application. Users will find state-of-the art information on the machinery for hyperspectral image acquisition, along with a critical assessment of the usage of hyperspectral imaging in diverse scientific fields. Provides a comprehensive roadmap of hyperspectral image analysis, with benefits and considerations for each method discussed Covers state-of-the-art applications in different scientific fields Discusses the implementation of hyperspectral devices in different environments
Raman microscopy is now well-established as one of the most powerful and versatile techniques for a diverse range of applications in both research and analytical laboratories. Its unique advantage is its ability to noninvasively characterize chemically complex and spatially inhomogeneous samples with a sub-micron spatial resolution. Modern confocal Raman scanning microscopy, which allows one to obtain two- and three-dimensional spectrochemical images of samples in various states and forms, has become a method of choice for a wide range of applications including the study of biological cells, tissues, and microorganisms, characterization of pharmaceutical drugs and formulations, forensic evidence, minerals and gems, carbon nanomaterials, semiconductors, composite polymers, and more. This book presents the techniques of confocal Raman microscopy and imaging for researchers and engineers from a variety of disciplines. It highlights the key aspects of this technique in order to effectively apply it in practice. It will appeal to a wide circle of readers who are interested in, or are already, using the methods of confocal Raman microscopy and imaging in their work, and will also be beneficial for novice Raman microscopy users.
Book Synopsis Modern Raman Microscopy by : Alexander Rzhevskii
Download or read book Modern Raman Microscopy written by Alexander Rzhevskii and published by Cambridge Scholars Publishing. This book was released on 2021-06-02 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Raman microscopy is now well-established as one of the most powerful and versatile techniques for a diverse range of applications in both research and analytical laboratories. Its unique advantage is its ability to noninvasively characterize chemically complex and spatially inhomogeneous samples with a sub-micron spatial resolution. Modern confocal Raman scanning microscopy, which allows one to obtain two- and three-dimensional spectrochemical images of samples in various states and forms, has become a method of choice for a wide range of applications including the study of biological cells, tissues, and microorganisms, characterization of pharmaceutical drugs and formulations, forensic evidence, minerals and gems, carbon nanomaterials, semiconductors, composite polymers, and more. This book presents the techniques of confocal Raman microscopy and imaging for researchers and engineers from a variety of disciplines. It highlights the key aspects of this technique in order to effectively apply it in practice. It will appeal to a wide circle of readers who are interested in, or are already, using the methods of confocal Raman microscopy and imaging in their work, and will also be beneficial for novice Raman microscopy users.
One of the first books devoted entirely to the subject of Raman microscopy, Raman Microscopy addresses issues of great interest to engineers working in Raman-microscope development and researchers concerned with areas ofapplication for this science. The book is written by several world recognized experts, who summarize the Raman effect before discussing the hardware and software involved in todays instruments. This format provides an excellent introduction to this up-and-coming discipline. All important applications, including those in materials science and earth science are covered in depth. Includes extensive description of the instrumentation, the Raman microspectrograph, the treatment of data, and micro-Raman imaging Examines the use of Raman microscopy in diverse applications, including some of the hyphenated methods Summarizes the Raman effect Discusses new uses for this technology
Book Synopsis Raman Microscopy by : George Turrell
Download or read book Raman Microscopy written by George Turrell and published by Academic Press. This book was released on 1996-06-24 with total page 493 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the first books devoted entirely to the subject of Raman microscopy, Raman Microscopy addresses issues of great interest to engineers working in Raman-microscope development and researchers concerned with areas ofapplication for this science. The book is written by several world recognized experts, who summarize the Raman effect before discussing the hardware and software involved in todays instruments. This format provides an excellent introduction to this up-and-coming discipline. All important applications, including those in materials science and earth science are covered in depth. Includes extensive description of the instrumentation, the Raman microspectrograph, the treatment of data, and micro-Raman imaging Examines the use of Raman microscopy in diverse applications, including some of the hyphenated methods Summarizes the Raman effect Discusses new uses for this technology
Chemical imaging and hyperspectral imaging modalities, which are non-destructive and non-invasive, are gaining a lot of interest in industrial and research fields. In particular, Raman spectroscopy and Raman hyperspectral imaging are rapidly becoming analytical methods of choice. High resolution and high fidelity Raman Imaging is challenging, however, due to the performance limitations of commercially available electronically tunable filters. The emphasis of my research has been to develop high resolution spectral filters and Raman imaging system for bio-materials. Raman scattering is commonly used for the nondestructive identification of minerals and polymers, as well as many other materials, and it is suitable for characterizing the degree of crystallinity, which can impart material properties such as piezoelectricity. Another type of inelastic scattering, Brillouin scattering, provides an indirect measure of sample elasticity and results from the interaction of light with time dependent optical density (refractive index) variations imparted by acoustic phonons. Achieving the necessary spectral resolution (bandwidth“1nm) to separate Rayleigh scattered light and Brillouin scattered light is challenging, and conventional grating monochromators are often replaced with a cascaded series of Fabry-Perot filters. Developing electronically tunable filters for Brillouin scattering is extremely challenging. A more recent technology, the virtually imaged phased array (VIPA), is capable of narrow passbands and high angular dispersion. As a part of this work generalized dispersion law for the VIPA, employing both anisotropic and isotropic dielectrics has been developed. Low light applications like Raman and Brillouin imaging techniques can take advantage of spatial multiplexing strategies in which the intensity of light from many pixel locations on the sample are detected simultaneously. Cyclic simplex matrices have been employed as encoding masks for binary state multiplexing applications and are employed in this work as part of the development of a multiplexed Hadamard transform Raman imaging system. The Hadamard transform Raman instrument utilizes an electro mechanically controllable digital micro-mirror device (DMD) as a spatial modulator. The result is a significant improvement in the signal-to-noise ratio of the Raman hyperspectral data which can enable faster acquisition times. In addition, correction methods have been developed to completely remove or reduce errors caused by photobleaching and stray cosmic radiation. In parallel with this work, novel signal processing and multivariate analyses have been investigated for the particularly challenging application of the Raman modality to biological samples. In particular, Raman markers have been identified using these methods to differentiate normal retinal tissue from glaucomatous tissue in mice. Glaucoma is a major neurodegenerative disease that often leads to irreversible vision loss. Its early detection relies on new analytical methods, such as Raman spectroscopy and Raman imaging that can reveal early biochemical changes associated with its onset.
Book Synopsis Development of a High Resolution Wavelength Filter and a Spatially Multiplexed Raman Imaging System by : Rajesh Morampudi
Download or read book Development of a High Resolution Wavelength Filter and a Spatially Multiplexed Raman Imaging System written by Rajesh Morampudi and published by . This book was released on 2014 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical imaging and hyperspectral imaging modalities, which are non-destructive and non-invasive, are gaining a lot of interest in industrial and research fields. In particular, Raman spectroscopy and Raman hyperspectral imaging are rapidly becoming analytical methods of choice. High resolution and high fidelity Raman Imaging is challenging, however, due to the performance limitations of commercially available electronically tunable filters. The emphasis of my research has been to develop high resolution spectral filters and Raman imaging system for bio-materials. Raman scattering is commonly used for the nondestructive identification of minerals and polymers, as well as many other materials, and it is suitable for characterizing the degree of crystallinity, which can impart material properties such as piezoelectricity. Another type of inelastic scattering, Brillouin scattering, provides an indirect measure of sample elasticity and results from the interaction of light with time dependent optical density (refractive index) variations imparted by acoustic phonons. Achieving the necessary spectral resolution (bandwidth“1nm) to separate Rayleigh scattered light and Brillouin scattered light is challenging, and conventional grating monochromators are often replaced with a cascaded series of Fabry-Perot filters. Developing electronically tunable filters for Brillouin scattering is extremely challenging. A more recent technology, the virtually imaged phased array (VIPA), is capable of narrow passbands and high angular dispersion. As a part of this work generalized dispersion law for the VIPA, employing both anisotropic and isotropic dielectrics has been developed. Low light applications like Raman and Brillouin imaging techniques can take advantage of spatial multiplexing strategies in which the intensity of light from many pixel locations on the sample are detected simultaneously. Cyclic simplex matrices have been employed as encoding masks for binary state multiplexing applications and are employed in this work as part of the development of a multiplexed Hadamard transform Raman imaging system. The Hadamard transform Raman instrument utilizes an electro mechanically controllable digital micro-mirror device (DMD) as a spatial modulator. The result is a significant improvement in the signal-to-noise ratio of the Raman hyperspectral data which can enable faster acquisition times. In addition, correction methods have been developed to completely remove or reduce errors caused by photobleaching and stray cosmic radiation. In parallel with this work, novel signal processing and multivariate analyses have been investigated for the particularly challenging application of the Raman modality to biological samples. In particular, Raman markers have been identified using these methods to differentiate normal retinal tissue from glaucomatous tissue in mice. Glaucoma is a major neurodegenerative disease that often leads to irreversible vision loss. Its early detection relies on new analytical methods, such as Raman spectroscopy and Raman imaging that can reveal early biochemical changes associated with its onset.