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MicroRNA Profiling

Author: Sweta Rani

Publisher: Springer Nature

Published: 2022-11-28

Total Pages: 258

ISBN-13: 1071628232

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This second edition provides updated and comprehensive methods on miRNA biogenesis and their role in the development and progression of various human diseases. Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MicroRNA Profiling: Methods and Protocols, Second Edition aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.

Book Synopsis MicroRNA Profiling by : Sweta Rani

Download or read book MicroRNA Profiling written by Sweta Rani and published by Springer Nature. This book was released on 2022-11-28 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition provides updated and comprehensive methods on miRNA biogenesis and their role in the development and progression of various human diseases. Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MicroRNA Profiling: Methods and Protocols, Second Edition aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.

MicroRNA Profiling in Cancer

Author: Yuriy Gusev

Publisher: CRC Press

Published: 2019-09-06

Total Pages: 257

ISBN-13: 9814267546

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This book presents current advances in the emerging interdisciplinary field of microRNA research of human cancers from a unique perspective of quantitative sciences: bioinformatics, computational and systems biology, and mathematical modeling. This volume contains adaptations and critical reviews of recent state-of-the-art studies, ranging from technological advances in microRNA detection and profiling, clinically oriented microRNA profiling in several human cancers, to a systems biology analysis of global patterns of microRNA regulation of signaling and metabolic pathways. Interactions with transcription factor regulatory networks and mathematical modeling of microRNA regulation are also discussed.

Book Synopsis MicroRNA Profiling in Cancer by : Yuriy Gusev

Download or read book MicroRNA Profiling in Cancer written by Yuriy Gusev and published by CRC Press. This book was released on 2019-09-06 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents current advances in the emerging interdisciplinary field of microRNA research of human cancers from a unique perspective of quantitative sciences: bioinformatics, computational and systems biology, and mathematical modeling. This volume contains adaptations and critical reviews of recent state-of-the-art studies, ranging from technological advances in microRNA detection and profiling, clinically oriented microRNA profiling in several human cancers, to a systems biology analysis of global patterns of microRNA regulation of signaling and metabolic pathways. Interactions with transcription factor regulatory networks and mathematical modeling of microRNA regulation are also discussed.

MicroRNA Expression Detection Methods

Author: Zhiguo Wang

Publisher: Springer

Published: 2009-12-23

Total Pages: 388

ISBN-13: 3642049281

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MicroRNAs (miRNAs), endogenous noncoding regulatory mRNAs of - nucleotides, have rapidly emerged as the central players in gene expression regulation. Owing to their ever-increasing implications in the control of various biological and pathological processes, miRNAs have now been considered novel biomarkers of various human diseases including, cancer, viral disease, cardiov- cular disorders, metabolic disturbances, etc. Particular expression pro?les have been associated with particular pathological states. Expression pro?ling of miR- NAs have therefore become extremely important not only for fundamentalists but also for clinicians. However, the methodologies used for detecting protein-coding mRNAs cannot be directly applied to miRNAs because of their small size. Over the past years, researchers have made great efforts to developing techniques suitable for miRNA detection and quanti?cation; a wide spectrum of creative and inno- tive techniques (more than 30 different methods) have been invented and validated. It has come to the time now to summarize these methods and present them in an orderly manner for better understanding and utilization of these methods to miRNA research and applications. In particular, the development of methods for quantifying circulating miRNAs opens up a fascinating opportunity for realizing miRNA as diagnostic and prognostic biomarkers of human disease. A book on this subject may help boosting up the passion of researchers to further improve the existing techniques and develop more new methods to ?t to new application needs. These considerations prompted us and urged us to undertake the work: writing a book focusing on miRNA expression detection methods.

Book Synopsis MicroRNA Expression Detection Methods by : Zhiguo Wang

Download or read book MicroRNA Expression Detection Methods written by Zhiguo Wang and published by Springer. This book was released on 2009-12-23 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: MicroRNAs (miRNAs), endogenous noncoding regulatory mRNAs of - nucleotides, have rapidly emerged as the central players in gene expression regulation. Owing to their ever-increasing implications in the control of various biological and pathological processes, miRNAs have now been considered novel biomarkers of various human diseases including, cancer, viral disease, cardiov- cular disorders, metabolic disturbances, etc. Particular expression pro?les have been associated with particular pathological states. Expression pro?ling of miR- NAs have therefore become extremely important not only for fundamentalists but also for clinicians. However, the methodologies used for detecting protein-coding mRNAs cannot be directly applied to miRNAs because of their small size. Over the past years, researchers have made great efforts to developing techniques suitable for miRNA detection and quanti?cation; a wide spectrum of creative and inno- tive techniques (more than 30 different methods) have been invented and validated. It has come to the time now to summarize these methods and present them in an orderly manner for better understanding and utilization of these methods to miRNA research and applications. In particular, the development of methods for quantifying circulating miRNAs opens up a fascinating opportunity for realizing miRNA as diagnostic and prognostic biomarkers of human disease. A book on this subject may help boosting up the passion of researchers to further improve the existing techniques and develop more new methods to ?t to new application needs. These considerations prompted us and urged us to undertake the work: writing a book focusing on miRNA expression detection methods.

Next-Generation MicroRNA Expression Profiling Technology

Author: Jian-Bing Fan

Publisher: Humana Press

Published: 2011-12-08

Total Pages: 341

ISBN-13: 9781617794261

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The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years. In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today. This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development. Written in the highly successful Methods in Molecular BiologyTM series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.

Book Synopsis Next-Generation MicroRNA Expression Profiling Technology by : Jian-Bing Fan

Download or read book Next-Generation MicroRNA Expression Profiling Technology written by Jian-Bing Fan and published by Humana Press. This book was released on 2011-12-08 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years. In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today. This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development. Written in the highly successful Methods in Molecular BiologyTM series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.

MicroRNA Detection and Target Identification

Author: Tamas Dalmay

Publisher: Springer Nature

Published: 2023-01-23

Total Pages: 267

ISBN-13: 1071629824

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This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Book Synopsis MicroRNA Detection and Target Identification by : Tamas Dalmay

Download or read book MicroRNA Detection and Target Identification written by Tamas Dalmay and published by Springer Nature. This book was released on 2023-01-23 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Activity-Based Protein Profiling

Author: Benjamin F. Cravatt

Publisher: Springer

Published: 2019-01-25

Total Pages: 417

ISBN-13: 3030111431

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This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.

Book Synopsis Activity-Based Protein Profiling by : Benjamin F. Cravatt

Download or read book Activity-Based Protein Profiling written by Benjamin F. Cravatt and published by Springer. This book was released on 2019-01-25 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.

Circulating microRNAs in Disease Diagnostics and their Potential Biological Relevance

Author: Peter Igaz

Publisher: Springer

Published: 2015-11-25

Total Pages: 292

ISBN-13: 3034809557

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MicroRNAs as the endogenous mediators of RNA interference have experienced an unprecedented career in recent years, highlighting their pathogenic, diagnostic and potential therapeutic relevance. Beside tissue microRNAs, they are also found in body fluids, most notably in blood. Significant differences of circulating microRNA levels have been found in various diseases, making them candidates for minimally invasive markers of disease, for example tumor malignancy. The book focuses on the potential diagnostic applicability of circulating microRNAs in various diseases and their potential biological significance.

Book Synopsis Circulating microRNAs in Disease Diagnostics and their Potential Biological Relevance by : Peter Igaz

Download or read book Circulating microRNAs in Disease Diagnostics and their Potential Biological Relevance written by Peter Igaz and published by Springer. This book was released on 2015-11-25 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: MicroRNAs as the endogenous mediators of RNA interference have experienced an unprecedented career in recent years, highlighting their pathogenic, diagnostic and potential therapeutic relevance. Beside tissue microRNAs, they are also found in body fluids, most notably in blood. Significant differences of circulating microRNA levels have been found in various diseases, making them candidates for minimally invasive markers of disease, for example tumor malignancy. The book focuses on the potential diagnostic applicability of circulating microRNAs in various diseases and their potential biological significance.

MicroRNAs as Tools in Biopharmaceutical Production

Author: Niall Barron

Publisher: Springer Science & Business Media

Published: 2012-11-15

Total Pages: 126

ISBN-13: 9400751281

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Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.

Book Synopsis MicroRNAs as Tools in Biopharmaceutical Production by : Niall Barron

Download or read book MicroRNAs as Tools in Biopharmaceutical Production written by Niall Barron and published by Springer Science & Business Media. This book was released on 2012-11-15 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.

Inherited Metabolic Disease in Adults

Author: Carla E. M. Hollak

Publisher: Oxford University Press

Published: 2016

Total Pages: 657

ISBN-13: 0199972133

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As clinical management of inherited metabolic diseases (IMDs) has improved, more patients affected by these conditions are surviving into adulthood. This trend, coupled with the widespread recognition that IMDs can present differently and for the first time during adulthood, makes the need for a working knowledge of these diseases more important than ever. Inherited Metabolic Disease in Adults offers an authoritative clinical guide to the adult manifestations of these challenging and myriad conditions. These include both the classic pediatric-onset conditions and a number of new diseases that can manifest at any age. It is the first book to give a clear and concise overview of how this group of conditions affects adult patients, a that topic will become a growing imperative for physicians across primary and specialized care.

Book Synopsis Inherited Metabolic Disease in Adults by : Carla E. M. Hollak

Download or read book Inherited Metabolic Disease in Adults written by Carla E. M. Hollak and published by Oxford University Press. This book was released on 2016 with total page 657 pages. Available in PDF, EPUB and Kindle. Book excerpt: As clinical management of inherited metabolic diseases (IMDs) has improved, more patients affected by these conditions are surviving into adulthood. This trend, coupled with the widespread recognition that IMDs can present differently and for the first time during adulthood, makes the need for a working knowledge of these diseases more important than ever. Inherited Metabolic Disease in Adults offers an authoritative clinical guide to the adult manifestations of these challenging and myriad conditions. These include both the classic pediatric-onset conditions and a number of new diseases that can manifest at any age. It is the first book to give a clear and concise overview of how this group of conditions affects adult patients, a that topic will become a growing imperative for physicians across primary and specialized care.

Microrna

Author: Xin Wang

Publisher:

Published: 2012

Total Pages:

ISBN-13:

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MicroRNAs (miRNAs) are a class of ~22 nt short, non-coding RNAs that post-transcriptionally regulate target mRNA expression. To date, ~2,000 mature miRNAs have been identified in humans and they are estimated to regulate about 50% of human genes. miRNAs, due to their ubiquitous target distribution, contribute to diverse processes including cell development, proliferation, differentiation, apoptosis, and metabolism. Dysregulation of miRNA expression has been reported in various cancers and metabolic disorders. miRNA are also implicated in the initiation and progression of those diseases. In my dissertation, I studied the differentially expressed (DE) miRNAs upon prostaglandin E2 (PGE2) stimulation in prostate cancer cells (PC-3). Concurrently I examined mRNA expression profile of the PC-3 system and determined anticorrelated miRNA:mRNA pairs. The DE miRNAs and their putative targets were affected by the induction of PGE2. They were suggested to be involved in PGE2 dysregulated signaling pathways in PC-3 prostate cancer. In the second part of the thesis work, I identified a set of adipose-enriched miRNAs from porcine tissues samples and verified that these miRNAs were conserved in humans. Adipose-enriched miRNAs were reported to be involved in metabolism, inflammation responses, and tumorigenesis. The analysis results of my thesis experiments suggested adipose-enriched miRNAs may have a potential role in connecting obesity, inflammation, and cancer. It is hoped that the understanding of the molecular basis in cancer and metabolic disorders on the miRNA level will provide new diagnostic targets and therapeutic targets for the diseases..

Book Synopsis Microrna by : Xin Wang

Download or read book Microrna written by Xin Wang and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: MicroRNAs (miRNAs) are a class of ~22 nt short, non-coding RNAs that post-transcriptionally regulate target mRNA expression. To date, ~2,000 mature miRNAs have been identified in humans and they are estimated to regulate about 50% of human genes. miRNAs, due to their ubiquitous target distribution, contribute to diverse processes including cell development, proliferation, differentiation, apoptosis, and metabolism. Dysregulation of miRNA expression has been reported in various cancers and metabolic disorders. miRNA are also implicated in the initiation and progression of those diseases. In my dissertation, I studied the differentially expressed (DE) miRNAs upon prostaglandin E2 (PGE2) stimulation in prostate cancer cells (PC-3). Concurrently I examined mRNA expression profile of the PC-3 system and determined anticorrelated miRNA:mRNA pairs. The DE miRNAs and their putative targets were affected by the induction of PGE2. They were suggested to be involved in PGE2 dysregulated signaling pathways in PC-3 prostate cancer. In the second part of the thesis work, I identified a set of adipose-enriched miRNAs from porcine tissues samples and verified that these miRNAs were conserved in humans. Adipose-enriched miRNAs were reported to be involved in metabolism, inflammation responses, and tumorigenesis. The analysis results of my thesis experiments suggested adipose-enriched miRNAs may have a potential role in connecting obesity, inflammation, and cancer. It is hoped that the understanding of the molecular basis in cancer and metabolic disorders on the miRNA level will provide new diagnostic targets and therapeutic targets for the diseases..