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Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Book Synopsis Molecular Biomethods Handbook by : John M. Walker
Download or read book Molecular Biomethods Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2008-09-04 with total page 1148 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
Book Synopsis Medical BioMethods Handbook by : John M. Walker
Download or read book Medical BioMethods Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2007-10-09 with total page 635 pages. Available in PDF, EPUB and Kindle. Book excerpt: John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Book Synopsis Molecular Biomethods Handbook by : John M. Walker
Download or read book Molecular Biomethods Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2008-11-04 with total page 1105 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.
Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition by : Leland J. Cseke
Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition written by Leland J. Cseke and published by CRC Press. This book was released on 2003-11-24 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.
Includes peer-reviewed life science and biomedical research protocols compiled from Methods in Molecular Biology, Methods in Molecular Medicine, Methods in Biotechnology, Methods in Pharmacology and Toxicology, Neuromethods, the Biomethods Handbook, the Proteomics Handbook, and Springer Laboratory Manuals.
Book Synopsis Springer Protocols by :
Download or read book Springer Protocols written by and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Includes peer-reviewed life science and biomedical research protocols compiled from Methods in Molecular Biology, Methods in Molecular Medicine, Methods in Biotechnology, Methods in Pharmacology and Toxicology, Neuromethods, the Biomethods Handbook, the Proteomics Handbook, and Springer Laboratory Manuals.
Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology
Book Synopsis Handbook of Molecular and Cellular Methods in Biology and Medicine by : Leland J. Cseke
Download or read book Handbook of Molecular and Cellular Methods in Biology and Medicine written by Leland J. Cseke and published by CRC Press. This book was released on 2016-04-19 with total page 735 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology
Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use
Book Synopsis Human Molecular Biology Laboratory Manual by : Stefan Surzycki
Download or read book Human Molecular Biology Laboratory Manual written by Stefan Surzycki and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use
This laboratory guide comes at a time when several other method books have already been published in this field. Is this one different from the others? Yes and no. There was no attempt made to be comprehensive. Rather, data were brought to bear on areas where enough competence has been gathered in our laboratories and to complement recent method books (many of which cover extensively various aspects of molecular biology) in those matters which appeared to us somewhat neglected. There was a constant preoccupation and effort to provide miniaturized proce dures that are both simple and time-saving. Interest was devoted to standardized procedures and culture conditions, avoiding dogmas such as those giving excessive importance to sophisticated culture media with endless adjustments for local or personal considerations. The key to success is the quality of the plant material serving as a source of cells. Consequently, isolation. extraction or culture techniques can be simplified and standardized. This is symptomatic for our times as it marks the end of a period when methodological matters were frequently above the biological problems. The times of "methods above all" is basically over, despite the fact that many of us still believe that, say, tissue culture is a "science" per se. By presenting a few original techniques we believe that one seriously reduces the empiricism still prevailing in this area of research.
Book Synopsis A Laboratory Guide for Cellular and Molecular Plant Biology by : I. Negrutiu
Download or read book A Laboratory Guide for Cellular and Molecular Plant Biology written by I. Negrutiu and published by Birkhäuser. This book was released on 2013-03-09 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide comes at a time when several other method books have already been published in this field. Is this one different from the others? Yes and no. There was no attempt made to be comprehensive. Rather, data were brought to bear on areas where enough competence has been gathered in our laboratories and to complement recent method books (many of which cover extensively various aspects of molecular biology) in those matters which appeared to us somewhat neglected. There was a constant preoccupation and effort to provide miniaturized proce dures that are both simple and time-saving. Interest was devoted to standardized procedures and culture conditions, avoiding dogmas such as those giving excessive importance to sophisticated culture media with endless adjustments for local or personal considerations. The key to success is the quality of the plant material serving as a source of cells. Consequently, isolation. extraction or culture techniques can be simplified and standardized. This is symptomatic for our times as it marks the end of a period when methodological matters were frequently above the biological problems. The times of "methods above all" is basically over, despite the fact that many of us still believe that, say, tissue culture is a "science" per se. By presenting a few original techniques we believe that one seriously reduces the empiricism still prevailing in this area of research.
Book Synopsis Protein and Cell Biomethods Handbook by :
Download or read book Protein and Cell Biomethods Handbook written by and published by . This book was released on 2008-01-01 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
The biological world operates on a multitude of scales - from molecules to tissues to organisms to ecosystems. Throughout these myriad levels runs a common thread: the communication and onward passage of information, from cell to cell, from organism to organism and ultimately, from generation to generation. But how does this information come alive to govern the processes that constitute life? The answer lies in the molecular components that cooperate through a series of carefully-regulated processes to bring the information in our genome to life. These components and processes lie at the heart of one of the most fascinating subjects to engage the minds of scientists today: molecular biology. Molecular Biology: Principles of Genome Function, Second Edition, offers a fresh approach to the teaching of molecular biology by focusing on the commonalities that exist between the three kingdoms of life, and discussing the differences between the three kingdoms to offer instructive insights into molecular processes and components. This gives students an accurate depiction of our current understanding of the conserved nature of molecular biology, and the differences that underpin biological diversity. Additionally, an integrated approach demonstrates how certain molecular phenomena have diverse impacts on genome function by presenting them as themes that recur throughout the book, rather than as artificially separated topics As an experimental science, molecular biology requires an appreciation for the approaches taken to yield the information from which concepts and principles are deduced. Experimental Approach panels throughout the text describe research that has been particularly valuable in elucidating difference aspects of molecular biology. Each panel is carefully cross-referenced to the discussion of key molecular biology tools and techniques, which are presented in a dedicated chapter at the end of the book. Molecular Biology further enriches the learning experience with full-color artwork, end-of-chapter questions and summaries, suggested further readings grouped by topic, and an extensive glossary of key terms. Features: A focus on the underlying principles of molecular biology equips students with a robust conceptual framework on which to build their knowledge An emphasis on their commonalities reflects the processes and components that exist between bacteria, archae, and eukaryotes Experimental Approach panels demonstrate the importance of experimental evidence by describing research that has been particularly valuable in the field
Book Synopsis Molecular Biology by : Nancy Craig
Download or read book Molecular Biology written by Nancy Craig and published by Oxford University Press. This book was released on 2014-05 with total page 945 pages. Available in PDF, EPUB and Kindle. Book excerpt: The biological world operates on a multitude of scales - from molecules to tissues to organisms to ecosystems. Throughout these myriad levels runs a common thread: the communication and onward passage of information, from cell to cell, from organism to organism and ultimately, from generation to generation. But how does this information come alive to govern the processes that constitute life? The answer lies in the molecular components that cooperate through a series of carefully-regulated processes to bring the information in our genome to life. These components and processes lie at the heart of one of the most fascinating subjects to engage the minds of scientists today: molecular biology. Molecular Biology: Principles of Genome Function, Second Edition, offers a fresh approach to the teaching of molecular biology by focusing on the commonalities that exist between the three kingdoms of life, and discussing the differences between the three kingdoms to offer instructive insights into molecular processes and components. This gives students an accurate depiction of our current understanding of the conserved nature of molecular biology, and the differences that underpin biological diversity. Additionally, an integrated approach demonstrates how certain molecular phenomena have diverse impacts on genome function by presenting them as themes that recur throughout the book, rather than as artificially separated topics As an experimental science, molecular biology requires an appreciation for the approaches taken to yield the information from which concepts and principles are deduced. Experimental Approach panels throughout the text describe research that has been particularly valuable in elucidating difference aspects of molecular biology. Each panel is carefully cross-referenced to the discussion of key molecular biology tools and techniques, which are presented in a dedicated chapter at the end of the book. Molecular Biology further enriches the learning experience with full-color artwork, end-of-chapter questions and summaries, suggested further readings grouped by topic, and an extensive glossary of key terms. Features: A focus on the underlying principles of molecular biology equips students with a robust conceptual framework on which to build their knowledge An emphasis on their commonalities reflects the processes and components that exist between bacteria, archae, and eukaryotes Experimental Approach panels demonstrate the importance of experimental evidence by describing research that has been particularly valuable in the field