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This book integrates the experimental procedures and theoretical principles for undergraduate, postgraduate, academicians and researchers in the area of agriculture and life sciences. The experiments have been updated and extended to reflect developments in the respective fields. In-text worked examples are again used to enhance student’s understanding of each topic. The book is designed to provide students with the experience of how scientists use their knowledge to understand real-world science related issues that confront them in their professional lives. Experimental procedures and troubleshooting of data are emphasised throughout the book. The book integrates theory and practices to ensure students understand why and how each technique is used.
Book Synopsis Molecular Methods in Plant Biology: A Comprehensive Book on Biotechnicological by : Krishnananda Pralhad Ingle
Download or read book Molecular Methods in Plant Biology: A Comprehensive Book on Biotechnicological written by Krishnananda Pralhad Ingle and published by I K International Pvt Ltd. This book was released on 2020-12-30 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book integrates the experimental procedures and theoretical principles for undergraduate, postgraduate, academicians and researchers in the area of agriculture and life sciences. The experiments have been updated and extended to reflect developments in the respective fields. In-text worked examples are again used to enhance student’s understanding of each topic. The book is designed to provide students with the experience of how scientists use their knowledge to understand real-world science related issues that confront them in their professional lives. Experimental procedures and troubleshooting of data are emphasised throughout the book. The book integrates theory and practices to ensure students understand why and how each technique is used.
Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods - such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.
Book Synopsis Methods in Plant Molecular Biology and Biotechnology by : Bernard R. Glick
Download or read book Methods in Plant Molecular Biology and Biotechnology written by Bernard R. Glick and published by CRC Press. This book was released on 2018-05-04 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods - such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.
Practical Applications of Plant Molecular Biology is an important new title which covers the major techniques and how they are applied to a range of vitally important areas. Divided broadly into four sections, this book covers key subjects including the identification of plants and plant pathogens using molecular techniques, the estimation of genetic variation in plants, the use of molecular markers in plant improvement and the use of plant transformation techniques for the improvement of quality and the introduction of resistance. Also included is a comprehensive listing and description of the most frequently used techniques and a set of appendices covering useful topics of reference for the reader. All undergraduates studying plant sciences, molecular biology, biotechnology and agricultural sciences would benefit from having access to this title as would those studying for upper-level Masters courses concentrating on the disciplines covered. This book also provides an invaluable source of reference for professionals in agriculture, plant breeding, crop protection and improvement, biotechnology and molecular biology.
Book Synopsis Practical Applications of Plant Molecular Biology by : Robert J. Henry
Download or read book Practical Applications of Plant Molecular Biology written by Robert J. Henry and published by Nelson Thornes. This book was released on 1997 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Practical Applications of Plant Molecular Biology is an important new title which covers the major techniques and how they are applied to a range of vitally important areas. Divided broadly into four sections, this book covers key subjects including the identification of plants and plant pathogens using molecular techniques, the estimation of genetic variation in plants, the use of molecular markers in plant improvement and the use of plant transformation techniques for the improvement of quality and the introduction of resistance. Also included is a comprehensive listing and description of the most frequently used techniques and a set of appendices covering useful topics of reference for the reader. All undergraduates studying plant sciences, molecular biology, biotechnology and agricultural sciences would benefit from having access to this title as would those studying for upper-level Masters courses concentrating on the disciplines covered. This book also provides an invaluable source of reference for professionals in agriculture, plant breeding, crop protection and improvement, biotechnology and molecular biology.
Modern plant science research currently integrates biochemistry and molecular biology. This book highlights recent trends in plant biotechnology and molecular genetics, serving as a working manual for scientists in academic, industrial, and federal laboratories. A wide variety of authors have contributed to this book, reflecting the thinking and expertise of active investigators who generate advances in technology. The authors were selected especially for their ability to create and/or implement novel research methods.
Book Synopsis Methods in Plant Biochemistry and Molecular Biology by : William V. Dashek
Download or read book Methods in Plant Biochemistry and Molecular Biology written by William V. Dashek and published by CRC Press. This book was released on 2018-05-04 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern plant science research currently integrates biochemistry and molecular biology. This book highlights recent trends in plant biotechnology and molecular genetics, serving as a working manual for scientists in academic, industrial, and federal laboratories. A wide variety of authors have contributed to this book, reflecting the thinking and expertise of active investigators who generate advances in technology. The authors were selected especially for their ability to create and/or implement novel research methods.
Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods - such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.
Book Synopsis Methods in Plant Molecular Biology and Biotechnology by : Bernard R. Glick
Download or read book Methods in Plant Molecular Biology and Biotechnology written by Bernard R. Glick and published by . This book was released on with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods - such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.
Examine the most recent developments in molecular plant pathology! This comprehensive reference book describes the molecular biology of plant-pathogen interactions in depth. With Dr. Vidhyasekaran’s keen insights and experienced critical viewpoint, Bacterial Disease Resistance in Plants: Molecular Biology and Biotechnological Applications not only presents reviews of current research but goes on to suggest future research strategies to exploit the studies in interventions with biotechnological, commercial, and field applications. This extraordinarily well-referenced book delivers in-depth examinations of: the molecular recognition process between plants and bacterial pathogens bacterial genes involved in the recognition process hrp, avr, dsp, and hsv genes the transcription of bacterial genes in plants signal transduction systems in bacteria and plants the functions of resistance genes and defense genes at the molecular level the elicitor molecules of bacterial pathogens and plants and their interactions plant and bacterial cell wall modifications and their role in triggering host defense mechanisms Bacterial Disease Resistance in Plants also explores active oxygen species, inducible plant proteins and their signals and transcription mechanisms, inducible secondary metabolites, and more. It introduces novel strategies for bacterial disease management using genes from human beings, birds, crabs, insects, fungi, bacteria, and bacteriophages; and genetic engineering techniques that can be used to develop transgenic, disease-resistant plants. Generously illustrated with figures and tables that make the data more quickly understandable, Bacterial Disease Resistance in Plants will be an invaluable resource and textbook for plant pathologists, bacteriologists, botanists, plant physiologists, plant molecular biologists, microbiologists, biochemists, plant cell and applied biologists, genetic engineers, and graduate-level students in these disciplines.
Book Synopsis Bacterial Disease Resistance in Plants by : P. Vidhyasekaran
Download or read book Bacterial Disease Resistance in Plants written by P. Vidhyasekaran and published by CRC Press. This book was released on 2002-10-23 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Examine the most recent developments in molecular plant pathology! This comprehensive reference book describes the molecular biology of plant-pathogen interactions in depth. With Dr. Vidhyasekaran’s keen insights and experienced critical viewpoint, Bacterial Disease Resistance in Plants: Molecular Biology and Biotechnological Applications not only presents reviews of current research but goes on to suggest future research strategies to exploit the studies in interventions with biotechnological, commercial, and field applications. This extraordinarily well-referenced book delivers in-depth examinations of: the molecular recognition process between plants and bacterial pathogens bacterial genes involved in the recognition process hrp, avr, dsp, and hsv genes the transcription of bacterial genes in plants signal transduction systems in bacteria and plants the functions of resistance genes and defense genes at the molecular level the elicitor molecules of bacterial pathogens and plants and their interactions plant and bacterial cell wall modifications and their role in triggering host defense mechanisms Bacterial Disease Resistance in Plants also explores active oxygen species, inducible plant proteins and their signals and transcription mechanisms, inducible secondary metabolites, and more. It introduces novel strategies for bacterial disease management using genes from human beings, birds, crabs, insects, fungi, bacteria, and bacteriophages; and genetic engineering techniques that can be used to develop transgenic, disease-resistant plants. Generously illustrated with figures and tables that make the data more quickly understandable, Bacterial Disease Resistance in Plants will be an invaluable resource and textbook for plant pathologists, bacteriologists, botanists, plant physiologists, plant molecular biologists, microbiologists, biochemists, plant cell and applied biologists, genetic engineers, and graduate-level students in these disciplines.
This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. The chapters in this book cover topics such as: microbial manipulation of auxin and cytokinins in plants; interplay between auxin and cytokinin and its impact on MAPK; H2O2 production in Arabidopsis leaves; crosstalk between jasmonate and auxin in plant stress responses of roots; and high-throughput protoplast trans-activation (PTA) screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Auxins and Cytokinins in Plant Biology: Methods and Protocols is a valuable resource to plant scientists, graduate and under graduate students in addressing their biological questions relevant to the functional implications of auxin and cytokinins.
Book Synopsis Auxins and Cytokinins in Plant Biology by : Thomas Dandekar
Download or read book Auxins and Cytokinins in Plant Biology written by Thomas Dandekar and published by Humana. This book was released on 2018-06-09 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. The chapters in this book cover topics such as: microbial manipulation of auxin and cytokinins in plants; interplay between auxin and cytokinin and its impact on MAPK; H2O2 production in Arabidopsis leaves; crosstalk between jasmonate and auxin in plant stress responses of roots; and high-throughput protoplast trans-activation (PTA) screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Auxins and Cytokinins in Plant Biology: Methods and Protocols is a valuable resource to plant scientists, graduate and under graduate students in addressing their biological questions relevant to the functional implications of auxin and cytokinins.
This laboratory guide comes at a time when several other method books have already been published in this field. Is this one different from the others? Yes and no. There was no attempt made to be comprehensive. Rather, data were brought to bear on areas where enough competence has been gathered in our laboratories and to complement recent method books (many of which cover extensively various aspects of molecular biology) in those matters which appeared to us somewhat neglected. There was a constant preoccupation and effort to provide miniaturized proce dures that are both simple and time-saving. Interest was devoted to standardized procedures and culture conditions, avoiding dogmas such as those giving excessive importance to sophisticated culture media with endless adjustments for local or personal considerations. The key to success is the quality of the plant material serving as a source of cells. Consequently, isolation. extraction or culture techniques can be simplified and standardized. This is symptomatic for our times as it marks the end of a period when methodological matters were frequently above the biological problems. The times of "methods above all" is basically over, despite the fact that many of us still believe that, say, tissue culture is a "science" per se. By presenting a few original techniques we believe that one seriously reduces the empiricism still prevailing in this area of research.
Book Synopsis A Laboratory Guide for Cellular and Molecular Plant Biology by : I. Negrutiu
Download or read book A Laboratory Guide for Cellular and Molecular Plant Biology written by I. Negrutiu and published by Birkhäuser. This book was released on 2013-03-09 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide comes at a time when several other method books have already been published in this field. Is this one different from the others? Yes and no. There was no attempt made to be comprehensive. Rather, data were brought to bear on areas where enough competence has been gathered in our laboratories and to complement recent method books (many of which cover extensively various aspects of molecular biology) in those matters which appeared to us somewhat neglected. There was a constant preoccupation and effort to provide miniaturized proce dures that are both simple and time-saving. Interest was devoted to standardized procedures and culture conditions, avoiding dogmas such as those giving excessive importance to sophisticated culture media with endless adjustments for local or personal considerations. The key to success is the quality of the plant material serving as a source of cells. Consequently, isolation. extraction or culture techniques can be simplified and standardized. This is symptomatic for our times as it marks the end of a period when methodological matters were frequently above the biological problems. The times of "methods above all" is basically over, despite the fact that many of us still believe that, say, tissue culture is a "science" per se. By presenting a few original techniques we believe that one seriously reduces the empiricism still prevailing in this area of research.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Book Synopsis Molecular Biology Techniques by : Heather Miller
Download or read book Molecular Biology Techniques written by Heather Miller and published by Academic Press. This book was released on 2011-10-18 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
Book Synopsis Plant Molecular Biology — A Laboratory Manual by : Melody S. Clark
Download or read book Plant Molecular Biology — A Laboratory Manual written by Melody S. Clark and published by Springer. This book was released on 2014-04-15 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
