Mycotoxin Protocols

Mycotoxin Protocols

Author: Mary W. Trucksess

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 245

ISBN-13: 1592590640

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Mycotoxins produced by molds are common contaminants of many important crops, including wheat, corn, rice, and peanuts. Some mycotoxins are found in fruits and vegetables. These contaminants have a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. The occurrence of mycotoxins in foods is an unavoidable worldwide problem. About 80 countries have imposed regulatory limits to minimize human and animal exposure to mycotoxins. Regulatory limits, including international standards, have tremendous economic impact and must be developed using science-based risk assessments. The purpose of Mycotoxin Protocols is to provide the scientific and technological basis for analytical methods for use in obtaining the exposure data needed for risk assessments. Mycotoxin Protocols is divided into four sections, which are interc- nected. The first section: Chapters 1–5 describe the general techniques for mycotoxin analysis with emphasis on the importance of method validation based on statistical parameters; sampling procedures for collecting a sample as representative as possible of a bulk lot; the isolation of mycotoxins for use as analytical standards or for toxicological studies; the evaluation of purity and preparation of standards; and the detection and identification of impu- ties in isolated mycotoxins. Sections 2–4: Chapters 6–19 describe the most current chromatographic and immunochemical methods for studies on the major mycotoxins.


Book Synopsis Mycotoxin Protocols by : Mary W. Trucksess

Download or read book Mycotoxin Protocols written by Mary W. Trucksess and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mycotoxins produced by molds are common contaminants of many important crops, including wheat, corn, rice, and peanuts. Some mycotoxins are found in fruits and vegetables. These contaminants have a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. The occurrence of mycotoxins in foods is an unavoidable worldwide problem. About 80 countries have imposed regulatory limits to minimize human and animal exposure to mycotoxins. Regulatory limits, including international standards, have tremendous economic impact and must be developed using science-based risk assessments. The purpose of Mycotoxin Protocols is to provide the scientific and technological basis for analytical methods for use in obtaining the exposure data needed for risk assessments. Mycotoxin Protocols is divided into four sections, which are interc- nected. The first section: Chapters 1–5 describe the general techniques for mycotoxin analysis with emphasis on the importance of method validation based on statistical parameters; sampling procedures for collecting a sample as representative as possible of a bulk lot; the isolation of mycotoxins for use as analytical standards or for toxicological studies; the evaluation of purity and preparation of standards; and the detection and identification of impu- ties in isolated mycotoxins. Sections 2–4: Chapters 6–19 describe the most current chromatographic and immunochemical methods for studies on the major mycotoxins.

Mycotoxins

Mycotoxins

Author: John F. Leslie

Publisher: CABI

Published: 2008

Total Pages: 496

ISBN-13: 1845930827

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This book is an outcome of the MycoGlobe conference in Accra. Most of the chapters are based on invited oral presentations made at the conference. The chapters in this book touch on issues including health, trade, ecology, epidemiology, occurrence, detection, management, awareness and policy. This book serves as a source of information on the occurrence and impact of mycotoxins on everything from trade and health to agricultural production in addition to suggesting opportunities for their management in Africa and elsewhere by researchers, policy makers and development investors.


Book Synopsis Mycotoxins by : John F. Leslie

Download or read book Mycotoxins written by John F. Leslie and published by CABI. This book was released on 2008 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is an outcome of the MycoGlobe conference in Accra. Most of the chapters are based on invited oral presentations made at the conference. The chapters in this book touch on issues including health, trade, ecology, epidemiology, occurrence, detection, management, awareness and policy. This book serves as a source of information on the occurrence and impact of mycotoxins on everything from trade and health to agricultural production in addition to suggesting opportunities for their management in Africa and elsewhere by researchers, policy makers and development investors.

Modern Methods in the Analysis and Structural Elucidation of Mycotoxins

Modern Methods in the Analysis and Structural Elucidation of Mycotoxins

Author: Richard J. Cole

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 487

ISBN-13: 0323148530

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Modern Methods in the Analysis and Structural Elucidation of Mycotoxins presents available methods of analysis and structural elucidation of mycotoxins by recognized experts in the various disciplines. The approach in each chapter of the book is to present each method initially in theoretical terms and then to review the method as it specifically applies to the analysis and/or structural elucidation of mycotoxins. Comprised of 15 chapters, the book's opening chapters deal with screening, sampling, and survey methods for mycotoxins and toxigenic fungi. This is followed by chapters dealing mostly with methods for structural elucidation, such as NMR and X-ray crystallography and IR and UV spectroscopy, as well as biosynthetic techniques. Significant chapters consider the analytical methods for mycotoxin analyses, including enzyme-linked immunosorbent assay system and tandem mass spectrometry. The concluding chapter examines the mycotoxin analytical problem in taxonomic or ecological terms. This book is of value to food and feed researchers, scientists, and manufacturers who are interested in product contamination control.


Book Synopsis Modern Methods in the Analysis and Structural Elucidation of Mycotoxins by : Richard J. Cole

Download or read book Modern Methods in the Analysis and Structural Elucidation of Mycotoxins written by Richard J. Cole and published by Elsevier. This book was released on 2012-12-02 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern Methods in the Analysis and Structural Elucidation of Mycotoxins presents available methods of analysis and structural elucidation of mycotoxins by recognized experts in the various disciplines. The approach in each chapter of the book is to present each method initially in theoretical terms and then to review the method as it specifically applies to the analysis and/or structural elucidation of mycotoxins. Comprised of 15 chapters, the book's opening chapters deal with screening, sampling, and survey methods for mycotoxins and toxigenic fungi. This is followed by chapters dealing mostly with methods for structural elucidation, such as NMR and X-ray crystallography and IR and UV spectroscopy, as well as biosynthetic techniques. Significant chapters consider the analytical methods for mycotoxin analyses, including enzyme-linked immunosorbent assay system and tandem mass spectrometry. The concluding chapter examines the mycotoxin analytical problem in taxonomic or ecological terms. This book is of value to food and feed researchers, scientists, and manufacturers who are interested in product contamination control.

Determining Mycotoxins and Mycotoxigenic Fungi in Food and Feed

Determining Mycotoxins and Mycotoxigenic Fungi in Food and Feed

Author: Sarah De Saeger

Publisher: Elsevier

Published: 2011-02-07

Total Pages: 452

ISBN-13: 0857090976

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Mycotoxins - toxic secondary metabolites produced by mycotoxigenic fungi – pose a significant risk to the food chain. Indeed, they may be the most hazardous of all food contaminants in terms of chronic toxicity and legislative limits on their levels in food and feed continue to be developed worldwide. Rapid and reliable methods for the determination of both mycotoxigenic fungi and mycotoxins in food and feed are therefore essential. This book reviews current and emerging methods in this area.Part one focuses on the essentials of mycotoxin determination, covering sampling, sample preparation and clean-up and key determination techniques, such as chromatographic separation, liquid chromatography-mass spectrometry and immunochemical methods. Part two then goes on to describe quality assurance, official methods and performance criteria for determining mycotoxins in food and feed. Topics covered include laboratory accreditation, method validation and measurement uncertainty. The development and analysis of biomarkers for mycotoxins are discussed in part three. Individual chapters focus on detecting exposure in humans and animals. Part four is concerned with the processes involved in determining mycotoxigenic fungi in food and feed. It also describes the identification of genes and gene clusters involved in mycotoxin synthesis, as well as DNA barcoding of toxigenic fungi. Finally, part five explores some of the emerging methods for mycotoxin analysis, ranging from bio-sensing to spectroscopic techniques.With its distinguished editor and international team of contributors, Determining mycotoxins and mycotoxigenic fungi in food and feed is a standard reference for all those concerned with reducing mycotoxin contamination in the food chain. Focuses on the essentials of mycotoxin determination, covering sampling, sample preparation, clean-up and key determination techniques Documents quality assurance and official methods and performance criteria for determining mycotoxins in food and feed Explores the processes of determining mycotoxigenic fungi in food and feed including the identification of genes and gene clusters


Book Synopsis Determining Mycotoxins and Mycotoxigenic Fungi in Food and Feed by : Sarah De Saeger

Download or read book Determining Mycotoxins and Mycotoxigenic Fungi in Food and Feed written by Sarah De Saeger and published by Elsevier. This book was released on 2011-02-07 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mycotoxins - toxic secondary metabolites produced by mycotoxigenic fungi – pose a significant risk to the food chain. Indeed, they may be the most hazardous of all food contaminants in terms of chronic toxicity and legislative limits on their levels in food and feed continue to be developed worldwide. Rapid and reliable methods for the determination of both mycotoxigenic fungi and mycotoxins in food and feed are therefore essential. This book reviews current and emerging methods in this area.Part one focuses on the essentials of mycotoxin determination, covering sampling, sample preparation and clean-up and key determination techniques, such as chromatographic separation, liquid chromatography-mass spectrometry and immunochemical methods. Part two then goes on to describe quality assurance, official methods and performance criteria for determining mycotoxins in food and feed. Topics covered include laboratory accreditation, method validation and measurement uncertainty. The development and analysis of biomarkers for mycotoxins are discussed in part three. Individual chapters focus on detecting exposure in humans and animals. Part four is concerned with the processes involved in determining mycotoxigenic fungi in food and feed. It also describes the identification of genes and gene clusters involved in mycotoxin synthesis, as well as DNA barcoding of toxigenic fungi. Finally, part five explores some of the emerging methods for mycotoxin analysis, ranging from bio-sensing to spectroscopic techniques.With its distinguished editor and international team of contributors, Determining mycotoxins and mycotoxigenic fungi in food and feed is a standard reference for all those concerned with reducing mycotoxin contamination in the food chain. Focuses on the essentials of mycotoxin determination, covering sampling, sample preparation, clean-up and key determination techniques Documents quality assurance and official methods and performance criteria for determining mycotoxins in food and feed Explores the processes of determining mycotoxigenic fungi in food and feed including the identification of genes and gene clusters

Anti-Mycotoxin Strategies for Food and Feed

Anti-Mycotoxin Strategies for Food and Feed

Author: Deepa Nagaraju

Publisher: John Wiley & Sons

Published: 2023-11-28

Total Pages: 292

ISBN-13: 1394160828

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Anti-Mycotoxin Strategies for Food and Feed Up-to-date information on mycotoxigenic fungi and toxins, with methods to control them in food and feed Anti-Mycotoxin Strategies for Food and Feed focuses on strategies to control the health risk of mycotoxins associated with cereals, pulses, and vegetables during post and pre harvest stages, in addition to foods, food-based products, and feeds. Each chapter is carefully designed to offer information elucidating various strategies, which include physical, chemical, and biological methods. The book provides a combined approach of advanced techniques used against mycotoxigenic fungi, which is helpful in studying the strategies for different mycotoxins. Special attention has been paid to diseases caused by mycotoxigenic fungi and their destructive effect on either preharvest, post-harvest, or storage, and their respective toxins which pose a long-term health risk to humans and animals, if contaminated food or feed enter our food chain. The primary focus of the text is on the recent and advanced approaches and strategies including control, detoxification, degradation and binding methods, with graphical representations of protocols with supporting tables and figures. Anti-Mycotoxin Strategies discusses specific sample topics such as: Advanced anti-fumonisin strategies and strategies for the control of aflatoxigenic Aspergillus species in contaminated food and feed Innovative strategies to decontaminate Ochratoxin A and Zearalenone. Effects of toxic citrinin, patulin and Ergot alkaloids in animals, birds, and humans, and effective mitigation strategies as its control measures. Detoxification and Decontamination strategies of Deoxynivalenol an emetic toxin, Trichothecenes, T-2/HT-2 toxin and nivalenol associated in food and feed as contaminants. Presenting and discussing mycotoxin management strategies at length and enabling readers to reduce or eliminate health hazards to humans and animals, Anti-Mycotoxin Strategies is an essential resource for mycologists, mycotoxicologists, Microbiologists, Food technology professionals in the food, seed, and feed industries, scientists, students, researchers, and farmers / agriculturists.


Book Synopsis Anti-Mycotoxin Strategies for Food and Feed by : Deepa Nagaraju

Download or read book Anti-Mycotoxin Strategies for Food and Feed written by Deepa Nagaraju and published by John Wiley & Sons. This book was released on 2023-11-28 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Anti-Mycotoxin Strategies for Food and Feed Up-to-date information on mycotoxigenic fungi and toxins, with methods to control them in food and feed Anti-Mycotoxin Strategies for Food and Feed focuses on strategies to control the health risk of mycotoxins associated with cereals, pulses, and vegetables during post and pre harvest stages, in addition to foods, food-based products, and feeds. Each chapter is carefully designed to offer information elucidating various strategies, which include physical, chemical, and biological methods. The book provides a combined approach of advanced techniques used against mycotoxigenic fungi, which is helpful in studying the strategies for different mycotoxins. Special attention has been paid to diseases caused by mycotoxigenic fungi and their destructive effect on either preharvest, post-harvest, or storage, and their respective toxins which pose a long-term health risk to humans and animals, if contaminated food or feed enter our food chain. The primary focus of the text is on the recent and advanced approaches and strategies including control, detoxification, degradation and binding methods, with graphical representations of protocols with supporting tables and figures. Anti-Mycotoxin Strategies discusses specific sample topics such as: Advanced anti-fumonisin strategies and strategies for the control of aflatoxigenic Aspergillus species in contaminated food and feed Innovative strategies to decontaminate Ochratoxin A and Zearalenone. Effects of toxic citrinin, patulin and Ergot alkaloids in animals, birds, and humans, and effective mitigation strategies as its control measures. Detoxification and Decontamination strategies of Deoxynivalenol an emetic toxin, Trichothecenes, T-2/HT-2 toxin and nivalenol associated in food and feed as contaminants. Presenting and discussing mycotoxin management strategies at length and enabling readers to reduce or eliminate health hazards to humans and animals, Anti-Mycotoxin Strategies is an essential resource for mycologists, mycotoxicologists, Microbiologists, Food technology professionals in the food, seed, and feed industries, scientists, students, researchers, and farmers / agriculturists.

Mycotoxigenic Fungi

Mycotoxigenic Fungi

Author: Antonio Moretti

Publisher: Humana

Published: 2016-12-06

Total Pages: 0

ISBN-13: 9781493967056

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This thorough volume explores the possibility of detecting and identifying toxigenic fungi, able to produce secondary metabolites known as mycotoxins, which cause severe health problems in humans and animals after exposure to contaminated food and feed, having a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. Beginning with a section on fungal genera and species of major significance along with their associated mycotoxins, the book continues with sections on Polymerase Chain Reaction (PCR)-based methods for the detection and identification of mycotoxigenic fungi, PCR-based methods for multiplex detection of mycotoxigenic fungi, as well as sections on combined approaches and new methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mycotoxigenic Fungi: Methods and Protocols will aid researchers working in this vital field to provide insight into possible actions to reduce mycotoxin contamination of crop plants and the food/feed byproducts.


Book Synopsis Mycotoxigenic Fungi by : Antonio Moretti

Download or read book Mycotoxigenic Fungi written by Antonio Moretti and published by Humana. This book was released on 2016-12-06 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thorough volume explores the possibility of detecting and identifying toxigenic fungi, able to produce secondary metabolites known as mycotoxins, which cause severe health problems in humans and animals after exposure to contaminated food and feed, having a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. Beginning with a section on fungal genera and species of major significance along with their associated mycotoxins, the book continues with sections on Polymerase Chain Reaction (PCR)-based methods for the detection and identification of mycotoxigenic fungi, PCR-based methods for multiplex detection of mycotoxigenic fungi, as well as sections on combined approaches and new methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mycotoxigenic Fungi: Methods and Protocols will aid researchers working in this vital field to provide insight into possible actions to reduce mycotoxin contamination of crop plants and the food/feed byproducts.

Mycotoxigenic Fungi

Mycotoxigenic Fungi

Author: Antonio Moretti

Publisher:

Published: 2016

Total Pages: 383

ISBN-13: 9781493967070

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This thorough volume explores the possibility of detecting and identifying toxigenic fungi, able to produce secondary metabolites known as mycotoxins, which cause severe health problems in humans and animals after exposure to contaminated food and feed, having a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. Beginning with a section on fungal genera and species of major significance along with their associated mycotoxins, the book continues with sections on Polymerase Chain Reaction (PCR)-based methods for the detection and identification of mycotoxigenic fungi, PCR-based methods for multiplex detection of mycotoxigenic fungi, as well as sections on combined approaches and new methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mycotoxigenic Fungi: Methods and Protocols will aid researchers working in this vital field to provide insight into possible actions to reduce mycotoxin contamination of crop plants and the food/feed byproducts.


Book Synopsis Mycotoxigenic Fungi by : Antonio Moretti

Download or read book Mycotoxigenic Fungi written by Antonio Moretti and published by . This book was released on 2016 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thorough volume explores the possibility of detecting and identifying toxigenic fungi, able to produce secondary metabolites known as mycotoxins, which cause severe health problems in humans and animals after exposure to contaminated food and feed, having a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. Beginning with a section on fungal genera and species of major significance along with their associated mycotoxins, the book continues with sections on Polymerase Chain Reaction (PCR)-based methods for the detection and identification of mycotoxigenic fungi, PCR-based methods for multiplex detection of mycotoxigenic fungi, as well as sections on combined approaches and new methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mycotoxigenic Fungi: Methods and Protocols will aid researchers working in this vital field to provide insight into possible actions to reduce mycotoxin contamination of crop plants and the food/feed byproducts.

Mycotoxin Control in Low- and Middle-Income Countries

Mycotoxin Control in Low- and Middle-Income Countries

Author: Christopher P. Wild

Publisher: IARC Working Group Report

Published: 2016-02-15

Total Pages: 0

ISBN-13: 9789283225096

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This book provides an evaluation of measures to reduce exposure to highly toxic and carcinogenic contaminants in staple diets in Africa as well as parts of Asia and Latin America. Many of the poorest people in these regions are exposed to the pervasive natural toxins, aflatoxins and fumonisins, on a daily basis by eating their staple diet of groundnuts, maize, and other cereals. Exposure to mycotoxins at these high levels substantially increases mortality and morbidity. Aflatoxins are a cause of human liver cancer, and fatalities from acute aflatoxin poisoning outbreaks occur in Africa and Asia. The International Agency for Research on Cancer convened a Working Group of world-leading experts to review the health effects of aflatoxins and fumonisins and to evaluate intervention measures. The panel concluded that these mycotoxins not only are a cause of acute poisoning and cancer but also are a likely contributor to the high levels of stunting in children in affected populations. The Working Group also identified effective measures to reduce exposure in developing countries. The panel evaluated 15 interventions, considering the strength of the evidence as well as its completeness and its transferability at an individual, community, or national level. Four of the interventions were judged to be ready for implementation: improvement of dietary diversity; crop sorting; post-harvest measures, including improved storage; and, in Latin America for maize, optimized nixtamalization. These recommendations would be relevant for investment of public, nongovernmental organization, and private funds at the scale of the subsistence farmer, the smallholder, and through to a more advanced value chain.


Book Synopsis Mycotoxin Control in Low- and Middle-Income Countries by : Christopher P. Wild

Download or read book Mycotoxin Control in Low- and Middle-Income Countries written by Christopher P. Wild and published by IARC Working Group Report. This book was released on 2016-02-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an evaluation of measures to reduce exposure to highly toxic and carcinogenic contaminants in staple diets in Africa as well as parts of Asia and Latin America. Many of the poorest people in these regions are exposed to the pervasive natural toxins, aflatoxins and fumonisins, on a daily basis by eating their staple diet of groundnuts, maize, and other cereals. Exposure to mycotoxins at these high levels substantially increases mortality and morbidity. Aflatoxins are a cause of human liver cancer, and fatalities from acute aflatoxin poisoning outbreaks occur in Africa and Asia. The International Agency for Research on Cancer convened a Working Group of world-leading experts to review the health effects of aflatoxins and fumonisins and to evaluate intervention measures. The panel concluded that these mycotoxins not only are a cause of acute poisoning and cancer but also are a likely contributor to the high levels of stunting in children in affected populations. The Working Group also identified effective measures to reduce exposure in developing countries. The panel evaluated 15 interventions, considering the strength of the evidence as well as its completeness and its transferability at an individual, community, or national level. Four of the interventions were judged to be ready for implementation: improvement of dietary diversity; crop sorting; post-harvest measures, including improved storage; and, in Latin America for maize, optimized nixtamalization. These recommendations would be relevant for investment of public, nongovernmental organization, and private funds at the scale of the subsistence farmer, the smallholder, and through to a more advanced value chain.

Methods in Molecular Biology: Mycotoxin protocols

Methods in Molecular Biology: Mycotoxin protocols

Author: John M. Walker

Publisher:

Published: 1984

Total Pages:

ISBN-13:

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Book Synopsis Methods in Molecular Biology: Mycotoxin protocols by : John M. Walker

Download or read book Methods in Molecular Biology: Mycotoxin protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Sampling Procedures to Detect Mycotoxins in Agricultural Commodities

Sampling Procedures to Detect Mycotoxins in Agricultural Commodities

Author: Thomas B. Whitaker

Publisher: Springer Science & Business Media

Published: 2010-09-22

Total Pages: 59

ISBN-13: 9048196345

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Adherence to regulatory limits for mycotoxins in agricultural commodities is important to safeguard consumers and to permit trade in affected commodities across international borders. Reliable estimates of mycotoxin concentrations are required to implement regulatory decisions on the suitability of lots of produce for consumption or trade. Effective schemes to test for mycotoxins depend not only upon sound analytical methods, but also on well designed and implemented sampling plans. This manual provides information to food analysts and regulatory officials on effective sampling plans to detect mycotoxins in food. The concepts of uncertainty and variability in mycotoxin test procedures are discussed as well as the importance of ensuring that samples are representative of the lot being sampled, and the consequences of a poorly designed sampling plan on the reliability of the measured levels of mycotoxins, possibly resulting in legal disputes and barriers to trade.


Book Synopsis Sampling Procedures to Detect Mycotoxins in Agricultural Commodities by : Thomas B. Whitaker

Download or read book Sampling Procedures to Detect Mycotoxins in Agricultural Commodities written by Thomas B. Whitaker and published by Springer Science & Business Media. This book was released on 2010-09-22 with total page 59 pages. Available in PDF, EPUB and Kindle. Book excerpt: Adherence to regulatory limits for mycotoxins in agricultural commodities is important to safeguard consumers and to permit trade in affected commodities across international borders. Reliable estimates of mycotoxin concentrations are required to implement regulatory decisions on the suitability of lots of produce for consumption or trade. Effective schemes to test for mycotoxins depend not only upon sound analytical methods, but also on well designed and implemented sampling plans. This manual provides information to food analysts and regulatory officials on effective sampling plans to detect mycotoxins in food. The concepts of uncertainty and variability in mycotoxin test procedures are discussed as well as the importance of ensuring that samples are representative of the lot being sampled, and the consequences of a poorly designed sampling plan on the reliability of the measured levels of mycotoxins, possibly resulting in legal disputes and barriers to trade.