Neuropeptide Techniques

Neuropeptide Techniques

Author: Illana Gozes

Publisher: Springer Science & Business Media

Published: 2007-11-13

Total Pages: 167

ISBN-13: 1588294684

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Neuropeptides exhibit multiple functions as hormones and regulators. As such, many techniques are required to understand the contribution and functions of neuropeptides in health and disease processes. Research in the neuropeptide field is aimed toward a better understanding of the physiology of the human body and toward the development of better diagnostics and therapeutics. This book will familiarize the audience with forefront techniques in the field.


Book Synopsis Neuropeptide Techniques by : Illana Gozes

Download or read book Neuropeptide Techniques written by Illana Gozes and published by Springer Science & Business Media. This book was released on 2007-11-13 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: Neuropeptides exhibit multiple functions as hormones and regulators. As such, many techniques are required to understand the contribution and functions of neuropeptides in health and disease processes. Research in the neuropeptide field is aimed toward a better understanding of the physiology of the human body and toward the development of better diagnostics and therapeutics. This book will familiarize the audience with forefront techniques in the field.

Methods in Neurotransmitter and Neuropeptide Research

Methods in Neurotransmitter and Neuropeptide Research

Author: S.H. Parvez

Publisher: Elsevier

Published: 2013-10-22

Total Pages: 436

ISBN-13: 1483290220

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This two-part book provides the most advanced biochemical and histochemical analytical methods on neurotransmitters, neuromodulators, and neuropeptides described by international specialists. The biochemical methods include liquid chromatography/electrochemistry, liquid chromatography/fluorescence and chemiluminescence detection, gas chromatography or liquid chromatography with mass spectrometry, and immunoassays. Morphological and physiological methods are also described, including immunohistochemistry, in situ hybridization, in vivo microdialysis, electrophysiological analysis, microwave irradiation, electrophysiological analysis, micro-anatomical dissection, peptide sequence analysis, quantitative autoradiographic analysis, and isolated CNS preparations. The neurotransmitters and neuropeptides include catecholamines, indoleamines, histamine, polyamines, and various neuropeptides, and their related enzymes. The book is the most comprehensive, advanced and versatile volume on the biochemical, morphological and physiological analysis of neurotransmitters and neuropeptides.


Book Synopsis Methods in Neurotransmitter and Neuropeptide Research by : S.H. Parvez

Download or read book Methods in Neurotransmitter and Neuropeptide Research written by S.H. Parvez and published by Elsevier. This book was released on 2013-10-22 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: This two-part book provides the most advanced biochemical and histochemical analytical methods on neurotransmitters, neuromodulators, and neuropeptides described by international specialists. The biochemical methods include liquid chromatography/electrochemistry, liquid chromatography/fluorescence and chemiluminescence detection, gas chromatography or liquid chromatography with mass spectrometry, and immunoassays. Morphological and physiological methods are also described, including immunohistochemistry, in situ hybridization, in vivo microdialysis, electrophysiological analysis, microwave irradiation, electrophysiological analysis, micro-anatomical dissection, peptide sequence analysis, quantitative autoradiographic analysis, and isolated CNS preparations. The neurotransmitters and neuropeptides include catecholamines, indoleamines, histamine, polyamines, and various neuropeptides, and their related enzymes. The book is the most comprehensive, advanced and versatile volume on the biochemical, morphological and physiological analysis of neurotransmitters and neuropeptides.

Neuropeptide Technology

Neuropeptide Technology

Author: P. Michael Conn

Publisher: Academic Press

Published: 2013-10-22

Total Pages: 589

ISBN-13: 1483268136

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Methods in Neurosciences, Volume 5: Neuropeptide Technology: Gene Expression and Neuropeptide Receptors discusses procedures and tools of assay useful for the identification, purification, and quantification of neuropeptides and their receptors. This volume is divided into two sections—neuropeptide gene expression and neuropeptide receptors. In these sections, this book specifically discusses the assays for peptide products of somatostatin gene expression; molecular assays for rat thyrotropin-releasing hormone gene; and structure, expression, and chromosomal localization of human gene. The gastrin-releasing peptide or mammalian bombesin; semiquantitative analysis of cellular somatostatin mRNA Levels; and atrial natriuretic factor receptor assays are also elaborated. This text likewise covers the receptors for opioid peptides in brain and autoradiography of somatostatin receptors in rat cerebellum. This publication is beneficial to neuroscientists and students intending to acquire knowledge of gene expression and neuropeptide receptors.


Book Synopsis Neuropeptide Technology by : P. Michael Conn

Download or read book Neuropeptide Technology written by P. Michael Conn and published by Academic Press. This book was released on 2013-10-22 with total page 589 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Neurosciences, Volume 5: Neuropeptide Technology: Gene Expression and Neuropeptide Receptors discusses procedures and tools of assay useful for the identification, purification, and quantification of neuropeptides and their receptors. This volume is divided into two sections—neuropeptide gene expression and neuropeptide receptors. In these sections, this book specifically discusses the assays for peptide products of somatostatin gene expression; molecular assays for rat thyrotropin-releasing hormone gene; and structure, expression, and chromosomal localization of human gene. The gastrin-releasing peptide or mammalian bombesin; semiquantitative analysis of cellular somatostatin mRNA Levels; and atrial natriuretic factor receptor assays are also elaborated. This text likewise covers the receptors for opioid peptides in brain and autoradiography of somatostatin receptors in rat cerebellum. This publication is beneficial to neuroscientists and students intending to acquire knowledge of gene expression and neuropeptide receptors.

Neuropeptide Y Protocols

Neuropeptide Y Protocols

Author: Ambikaipakan Balasubramaniam

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 244

ISBN-13: 159259042X

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The observation that neuropeptide Y (NPY) is the most abundant peptide present in the mammalian nervous system and the finding that it elicits the most powerful orexigenic signal have led to active investigations of the properties of the NPY family of hormones, including peptide YY (PYY) and pancreatic polypeptide (PP). Nearly two decades of research have led to the identification of several NPY receptor subtypes and the development of useful receptor selective ligands. Moreover, these investigations have imp- cated NPY in the pathophysiology of a number of diseases, including feeding disorders, seizures, memory loss, anxiety, depression, and heart failure. Vigorous efforts are therefore continuing, not only to understand the bioche- cal aspects of NPY actions, but also toward developing NPY-based treatments for a variety of disorders. To facilitate these efforts, it was decided to produce the first handbook on NPY research techniques as part of the Methods in Molecular Biology Series. In compiling Neuropeptide Y Protocols, I have gathered contributions on techniques considered critical for the advancement of the NPY field from experts in various disciplines. Each chapter starts with a brief introduction, with Materials and Methods sections following. The latter sections are presented in an easy to follow step-by-step format. The last section of the chapter, Notes, highlights pitfalls and the maneuvers employed to overcome them. This information, not usually disseminated in standard research pub- cations, may prove extremely useful for investigators employing these te- niques in NYP research.


Book Synopsis Neuropeptide Y Protocols by : Ambikaipakan Balasubramaniam

Download or read book Neuropeptide Y Protocols written by Ambikaipakan Balasubramaniam and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: The observation that neuropeptide Y (NPY) is the most abundant peptide present in the mammalian nervous system and the finding that it elicits the most powerful orexigenic signal have led to active investigations of the properties of the NPY family of hormones, including peptide YY (PYY) and pancreatic polypeptide (PP). Nearly two decades of research have led to the identification of several NPY receptor subtypes and the development of useful receptor selective ligands. Moreover, these investigations have imp- cated NPY in the pathophysiology of a number of diseases, including feeding disorders, seizures, memory loss, anxiety, depression, and heart failure. Vigorous efforts are therefore continuing, not only to understand the bioche- cal aspects of NPY actions, but also toward developing NPY-based treatments for a variety of disorders. To facilitate these efforts, it was decided to produce the first handbook on NPY research techniques as part of the Methods in Molecular Biology Series. In compiling Neuropeptide Y Protocols, I have gathered contributions on techniques considered critical for the advancement of the NPY field from experts in various disciplines. Each chapter starts with a brief introduction, with Materials and Methods sections following. The latter sections are presented in an easy to follow step-by-step format. The last section of the chapter, Notes, highlights pitfalls and the maneuvers employed to overcome them. This information, not usually disseminated in standard research pub- cations, may prove extremely useful for investigators employing these te- niques in NYP research.

Neuropeptide Technology

Neuropeptide Technology

Author: P. Michael Conn

Publisher: Academic Press

Published: 2013-10-22

Total Pages: 440

ISBN-13: 1483259498

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Methods in Neurosciences, Volume 6: Neuropeptide Technology: Synthesis, Assay, Purification, and Processing describes procedures and tools of assay useful for the identification, purification, and quantification of neuropeptides and their receptors. This volume is divided into four sections— chemical synthesis and biosynthesis; measurement of neuropeptides; purification and characterization; and neuropeptide degrading and processing enzymes. In these sections, this book specifically discusses the synthesis of peptide substrates for protein kinase C; synthesis of glycosyl neuropeptides; and ultrastructural localization of peptides. The measurement of neurokinin B by radioimmunoassay; purification and characterization of neuroendocrine peptides from rat brain; and preparation of glia maturation factor ß are also elaborated. This text likewise covers the assays for arginine/lysine carboxypeptidases and enzymes that metabolize atrial natriuretic peptide. This publication is beneficial to neuroscientists and students researching on the synthesis, assay, purification, and processing of neuropeptides.


Book Synopsis Neuropeptide Technology by : P. Michael Conn

Download or read book Neuropeptide Technology written by P. Michael Conn and published by Academic Press. This book was released on 2013-10-22 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Neurosciences, Volume 6: Neuropeptide Technology: Synthesis, Assay, Purification, and Processing describes procedures and tools of assay useful for the identification, purification, and quantification of neuropeptides and their receptors. This volume is divided into four sections— chemical synthesis and biosynthesis; measurement of neuropeptides; purification and characterization; and neuropeptide degrading and processing enzymes. In these sections, this book specifically discusses the synthesis of peptide substrates for protein kinase C; synthesis of glycosyl neuropeptides; and ultrastructural localization of peptides. The measurement of neurokinin B by radioimmunoassay; purification and characterization of neuroendocrine peptides from rat brain; and preparation of glia maturation factor ß are also elaborated. This text likewise covers the assays for arginine/lysine carboxypeptidases and enzymes that metabolize atrial natriuretic peptide. This publication is beneficial to neuroscientists and students researching on the synthesis, assay, purification, and processing of neuropeptides.

Neuropeptide Analogs, Conjugates, and Fragments

Neuropeptide Analogs, Conjugates, and Fragments

Author: P. Michael Conn

Publisher: Academic Press

Published: 2013-10-22

Total Pages: 415

ISBN-13: 1483217531

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Methods in Neurosciences, Volume 13: Neuropeptide Analogs, Conjugates, and Fragments covers the synthesis and characterization of peptide analogs, conjugates, and fragments, their use as ligands for receptors, and their role in the development and use of antisera. The book discusses techniques such as novel synthetic approaches; biotinylation; purification and characterization; radioligand techniques and assay development; use of agonists and antagonists; distinguishing receptor subtypes; conjugation to carrier proteins; antiidiotypic antibody development; and radiolabeling. Neuroscientists, biochemists, neurochemists, and pharmacologists will find the book useful.


Book Synopsis Neuropeptide Analogs, Conjugates, and Fragments by : P. Michael Conn

Download or read book Neuropeptide Analogs, Conjugates, and Fragments written by P. Michael Conn and published by Academic Press. This book was released on 2013-10-22 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Neurosciences, Volume 13: Neuropeptide Analogs, Conjugates, and Fragments covers the synthesis and characterization of peptide analogs, conjugates, and fragments, their use as ligands for receptors, and their role in the development and use of antisera. The book discusses techniques such as novel synthetic approaches; biotinylation; purification and characterization; radioligand techniques and assay development; use of agonists and antagonists; distinguishing receptor subtypes; conjugation to carrier proteins; antiidiotypic antibody development; and radiolabeling. Neuroscientists, biochemists, neurochemists, and pharmacologists will find the book useful.

Neuropeptides

Neuropeptides

Author: Adalberto Merighi

Publisher: Humana Press

Published: 2017-04-30

Total Pages: 416

ISBN-13: 9781493962112

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Since the early days, the field of neuropeptide biology has dramatically widened, and today the ultimate frontiers in neuropeptide research lie in the development of pharmacologically active compounds that are capable of crossing the blood-brain barrier to exert their biological role(s) in vivo and in the construction of genetic vectors to be employed in gene therapy. Neuropeptides: Methods and Protocols presents a readily reproducible collection of established and emerging techniques for neuropeptide research as contributed by expert researchers in the field. The detailed methods presented cover subjects such as immunocytochemical localization, biochemical characterization, functional analysis, development and production of genetic probes, and the design of neuropeptide derivatives for cellular neurobiology as well as the potential therapeutic applications. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to follow, Neuropeptides: Methods and Protocols is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, and electrophysiologists that are currently active in the field or are willing to enter this exciting and still expanding area of neurobiology.


Book Synopsis Neuropeptides by : Adalberto Merighi

Download or read book Neuropeptides written by Adalberto Merighi and published by Humana Press. This book was released on 2017-04-30 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the early days, the field of neuropeptide biology has dramatically widened, and today the ultimate frontiers in neuropeptide research lie in the development of pharmacologically active compounds that are capable of crossing the blood-brain barrier to exert their biological role(s) in vivo and in the construction of genetic vectors to be employed in gene therapy. Neuropeptides: Methods and Protocols presents a readily reproducible collection of established and emerging techniques for neuropeptide research as contributed by expert researchers in the field. The detailed methods presented cover subjects such as immunocytochemical localization, biochemical characterization, functional analysis, development and production of genetic probes, and the design of neuropeptide derivatives for cellular neurobiology as well as the potential therapeutic applications. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to follow, Neuropeptides: Methods and Protocols is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, and electrophysiologists that are currently active in the field or are willing to enter this exciting and still expanding area of neurobiology.

Neuropeptide Protocols

Neuropeptide Protocols

Author: G. Brent Irvine

Publisher: Springer Science & Business Media

Published: 1997

Total Pages: 408

ISBN-13:

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Leading scientists offer an unprecedented suite of master protocols for analyzing neuropeptide structure and function using optimized classic methods and the latest state-of-the-art techniques. This comprehensive collection includes valuable techniques for extracting and purifying neuropeptides from biological sources, for their sequence determination by Edman degradation and PCR, and for the solid-phase synthesis and purification of peptides and peptide analogs. Also covered are physical techniques for the characterization of synthetic or natural peptides, methods for immunocytochemical sample preparation and visualization at both light and electron levels, and techniques for neuropeptide-receptor interaction. Chapters on molecular modeling, radioisotopic labeling, production of antisera, the measurement of efflux from brain to blood, and the assay and inhibition of neuropeptidases complete this indispensable collection of readily reproducible protocols.


Book Synopsis Neuropeptide Protocols by : G. Brent Irvine

Download or read book Neuropeptide Protocols written by G. Brent Irvine and published by Springer Science & Business Media. This book was released on 1997 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading scientists offer an unprecedented suite of master protocols for analyzing neuropeptide structure and function using optimized classic methods and the latest state-of-the-art techniques. This comprehensive collection includes valuable techniques for extracting and purifying neuropeptides from biological sources, for their sequence determination by Edman degradation and PCR, and for the solid-phase synthesis and purification of peptides and peptide analogs. Also covered are physical techniques for the characterization of synthetic or natural peptides, methods for immunocytochemical sample preparation and visualization at both light and electron levels, and techniques for neuropeptide-receptor interaction. Chapters on molecular modeling, radioisotopic labeling, production of antisera, the measurement of efflux from brain to blood, and the assay and inhibition of neuropeptidases complete this indispensable collection of readily reproducible protocols.

Neuropeptide Protocols. Methods in Molecular Biology

Neuropeptide Protocols. Methods in Molecular Biology

Author: G. Brent Irvine

Publisher:

Published: 1997

Total Pages: 386

ISBN-13: 9781280836794

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Leading scientists offer an unprecedented suite of master protocols for analyzing neuropeptide structure and function using optimized classic methods and the latest state-of-the-art techniques. This comprehensive collection includes valuable techniques for extracting and purifying neuropeptides from biological sources, for their sequence determination by Edman degradation and PCR, and for the solid-phase synthesis and purification of peptides and peptide analogs. Also covered are physical techniques for the characterization of synthetic or natural peptides, methods for immunocytochemical sample preparation and visualization at both light and electron levels, and techniques for neuropeptide-receptor interaction. Chapters on molecular modeling, radioisotopic labeling, production of antisera, the measurement of efflux from brain to blood, and the assay and inhibition of neuropeptidases complete this indispensable collection of readily reproducible protocols.


Book Synopsis Neuropeptide Protocols. Methods in Molecular Biology by : G. Brent Irvine

Download or read book Neuropeptide Protocols. Methods in Molecular Biology written by G. Brent Irvine and published by . This book was released on 1997 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading scientists offer an unprecedented suite of master protocols for analyzing neuropeptide structure and function using optimized classic methods and the latest state-of-the-art techniques. This comprehensive collection includes valuable techniques for extracting and purifying neuropeptides from biological sources, for their sequence determination by Edman degradation and PCR, and for the solid-phase synthesis and purification of peptides and peptide analogs. Also covered are physical techniques for the characterization of synthetic or natural peptides, methods for immunocytochemical sample preparation and visualization at both light and electron levels, and techniques for neuropeptide-receptor interaction. Chapters on molecular modeling, radioisotopic labeling, production of antisera, the measurement of efflux from brain to blood, and the assay and inhibition of neuropeptidases complete this indispensable collection of readily reproducible protocols.

Neuropeptides in Neuroprotection and Neuroregeneration

Neuropeptides in Neuroprotection and Neuroregeneration

Author: Fred Nyberg

Publisher: CRC Press

Published: 2012-06-19

Total Pages: 321

ISBN-13: 1439830622

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Although the genomic era is no longer in its infancy, the life sciences are still facing questions about the role of endogenous proteins and peptides in homeostasis and pathologies. Delving into one of the most current fields of interest in biology and medicine, Neuropeptides in Neuroprotection and Neuroregeneration describes the impact of neuropeptides on neuroprotection and neuroregeneration. The book begins with chapters describing important features of the endogenous neuropeptide systems related to their formation, receptor signaling, and inactivation. It includes chapters focused on the design and development of peptide-like drugs (peptidomimetics). In addition, the book covers: General aspects regarding the biosynthesis, structures, and distribution of neuroactive peptides and their receptors Basic mechanisms for neuropeptide action, metabolism, as well as techniques for their detection and aspects essential for the cellular mechanisms underlying brain pathology Neuropeptides known for their impact in neurodegenerative and neuroprotective processes Fundamental aspects as well as recent progress in the development of peptidomimetics of neuroprotective and cognition-enhancing peptides


Book Synopsis Neuropeptides in Neuroprotection and Neuroregeneration by : Fred Nyberg

Download or read book Neuropeptides in Neuroprotection and Neuroregeneration written by Fred Nyberg and published by CRC Press. This book was released on 2012-06-19 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although the genomic era is no longer in its infancy, the life sciences are still facing questions about the role of endogenous proteins and peptides in homeostasis and pathologies. Delving into one of the most current fields of interest in biology and medicine, Neuropeptides in Neuroprotection and Neuroregeneration describes the impact of neuropeptides on neuroprotection and neuroregeneration. The book begins with chapters describing important features of the endogenous neuropeptide systems related to their formation, receptor signaling, and inactivation. It includes chapters focused on the design and development of peptide-like drugs (peptidomimetics). In addition, the book covers: General aspects regarding the biosynthesis, structures, and distribution of neuroactive peptides and their receptors Basic mechanisms for neuropeptide action, metabolism, as well as techniques for their detection and aspects essential for the cellular mechanisms underlying brain pathology Neuropeptides known for their impact in neurodegenerative and neuroprotective processes Fundamental aspects as well as recent progress in the development of peptidomimetics of neuroprotective and cognition-enhancing peptides