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Book Synopsis Optical Microscopic and Spectroscopic Techniques Targeting Biological Applications by : Vicente Micó
Download or read book Optical Microscopic and Spectroscopic Techniques Targeting Biological Applications written by Vicente Micó and published by Frontiers Media SA. This book was released on 2021-10-22 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Optical Techniques in Biological Research discusses the fundamentals and applications of light scattering, Raman scattering, Fourier transform infrared spectroscopy, nanosecond fluorescence spectroscopy, and circular dichroism. Electron microscopy is also included owing to some of its classical similarity to optical microscopy optical structural and resonance techniques for biological research. The chapters are aimed at a level such that only a general understanding of chemistry and biology is required. The objective is to present material in a way that allows the research worker to assess quickly the applicability, utility, and significance of the specific technique to his or her problem or field of interest. Together with Structural and Resonance Techniques in Biological Research, this book marks the introduction of a new series of volumes, Physical Techniques in Biology and Medicine, which is intended to replace a previous treatise, Physical Techniques in Biological Research.
Book Synopsis Optical Techniques in Biological Research by : Denis Rousseau
Download or read book Optical Techniques in Biological Research written by Denis Rousseau and published by Elsevier. This book was released on 2012-12-02 with total page 443 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical Techniques in Biological Research discusses the fundamentals and applications of light scattering, Raman scattering, Fourier transform infrared spectroscopy, nanosecond fluorescence spectroscopy, and circular dichroism. Electron microscopy is also included owing to some of its classical similarity to optical microscopy optical structural and resonance techniques for biological research. The chapters are aimed at a level such that only a general understanding of chemistry and biology is required. The objective is to present material in a way that allows the research worker to assess quickly the applicability, utility, and significance of the specific technique to his or her problem or field of interest. Together with Structural and Resonance Techniques in Biological Research, this book marks the introduction of a new series of volumes, Physical Techniques in Biology and Medicine, which is intended to replace a previous treatise, Physical Techniques in Biological Research.
This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand ing for the biochemist or biologist who needs to collaborate with spe cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc ture using circular dichroism and Fourier-transform infrared spec troscopy.
Book Synopsis Microscopy, Optical Spectroscopy, and Macroscopic Techniques by : Christopher Jones
Download or read book Microscopy, Optical Spectroscopy, and Macroscopic Techniques written by Christopher Jones and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand ing for the biochemist or biologist who needs to collaborate with spe cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc ture using circular dichroism and Fourier-transform infrared spec troscopy.
This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.
Book Synopsis Optical Spectroscopic and Microscopic Techniques by : Harekrushna Sahoo
Download or read book Optical Spectroscopic and Microscopic Techniques written by Harekrushna Sahoo and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.
The last few years have seen an upsurge of interest in the study of cells by optical microscopy. The advent of new techniques such as confocal microscopy and the availability of extremely sensitive digital imaging devices are revolutioniz-ing the field. A number of groups have developed new ways of making spectroscopic measurements at the microscopic level, accompanied by the introduction of appropriate sen-sor molecules for cellular assays. The aim of this volume will be to bring together the various advances in order to provide the reader with an up-to-date account of what can now be achieved with modern optical microscopic methods.
Book Synopsis New Techniques of Optical Microscopy and Microspectroscopy by : Cherry
Download or read book New Techniques of Optical Microscopy and Microspectroscopy written by Cherry and published by CRC Press. This book was released on 1991 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last few years have seen an upsurge of interest in the study of cells by optical microscopy. The advent of new techniques such as confocal microscopy and the availability of extremely sensitive digital imaging devices are revolutioniz-ing the field. A number of groups have developed new ways of making spectroscopic measurements at the microscopic level, accompanied by the introduction of appropriate sen-sor molecules for cellular assays. The aim of this volume will be to bring together the various advances in order to provide the reader with an up-to-date account of what can now be achieved with modern optical microscopic methods.
For a host of reasons, nonlinear optical spectroscopy is a valuable tool for biochemical applications where minimally invasive diagnostics is desired. Biochemical Applications of Nonlinear Optical Spectroscopy presents the latest technological advances and offers a perspective on future directions in this important field. Written by an international panel of experts, this volume begins with a comparison of nonlinear optical spectroscopy and x-ray crystallography. The text examines the use of multiphoton fluorescence to study chemical phenomena in the skin, the use of nonlinear optics to enhance traditional optical spectroscopy, and the multimodal approach, which incorporates several spectroscopic techniques in one instrument. Later chapters explore Raman microscopy, third-harmonic generation microscopy, and non-linear Raman microspectroscopy. The text explores the promise of beam shaping and the use of broadband laser pulse generated through continuum generation and an optical pulse shaper. Lastly, the book discusses the effects of spatial beam shaping on the generated nonlinear Raman signals in a tightly focused geometry and provides insight into the extension of nonlinear optical spectroscopy to the nanoscale through the use of plasmonic tip-enhanced arrangement. With novel experimental approaches to this technology expanding day-by-day, the book’s balanced coverage from a wide range of international contributors not only elucidates important achievements, but also outlines future directions in this dynamic and promising field.
Book Synopsis Biochemical Applications of Nonlinear Optical Spectroscopy by : Vladislav V. Yakovlev
Download or read book Biochemical Applications of Nonlinear Optical Spectroscopy written by Vladislav V. Yakovlev and published by CRC Press. This book was released on 2018-10-08 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: For a host of reasons, nonlinear optical spectroscopy is a valuable tool for biochemical applications where minimally invasive diagnostics is desired. Biochemical Applications of Nonlinear Optical Spectroscopy presents the latest technological advances and offers a perspective on future directions in this important field. Written by an international panel of experts, this volume begins with a comparison of nonlinear optical spectroscopy and x-ray crystallography. The text examines the use of multiphoton fluorescence to study chemical phenomena in the skin, the use of nonlinear optics to enhance traditional optical spectroscopy, and the multimodal approach, which incorporates several spectroscopic techniques in one instrument. Later chapters explore Raman microscopy, third-harmonic generation microscopy, and non-linear Raman microspectroscopy. The text explores the promise of beam shaping and the use of broadband laser pulse generated through continuum generation and an optical pulse shaper. Lastly, the book discusses the effects of spatial beam shaping on the generated nonlinear Raman signals in a tightly focused geometry and provides insight into the extension of nonlinear optical spectroscopy to the nanoscale through the use of plasmonic tip-enhanced arrangement. With novel experimental approaches to this technology expanding day-by-day, the book’s balanced coverage from a wide range of international contributors not only elucidates important achievements, but also outlines future directions in this dynamic and promising field.
Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.
Book Synopsis Fluorescence Microscopy by : Ulrich Kubitscheck
Download or read book Fluorescence Microscopy written by Ulrich Kubitscheck and published by John Wiley & Sons. This book was released on 2017-06-19 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.
This multi-author contributed volume gives a comprehensive overview of recent progress in various vibrational spectroscopic techniques and chemometric methods and their applications in chemistry, biology and medicine. In order to meet the needs of readers, the book focuses on recent advances in technical development and potential exploitations of the theory, as well as the new applications of vibrational methods to problems of recent general interest that were difficult or even impossible to achieve in the not so distant past. Integrating vibrational spectroscopy and computational approaches serves as a handbook for people performing vibrational spectroscopy followed by chemometric analysis hence both experimental methods as well as procedures of recommended analysis are described. This volume is written for individuals who develop new methodologies and extend these applications to new realms of chemical and medicinal interest.
Book Synopsis Optical Spectroscopy and Computational Methods in Biology and Medicine by : Malgorzata Baranska
Download or read book Optical Spectroscopy and Computational Methods in Biology and Medicine written by Malgorzata Baranska and published by Springer Science & Business Media. This book was released on 2013-12-05 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: This multi-author contributed volume gives a comprehensive overview of recent progress in various vibrational spectroscopic techniques and chemometric methods and their applications in chemistry, biology and medicine. In order to meet the needs of readers, the book focuses on recent advances in technical development and potential exploitations of the theory, as well as the new applications of vibrational methods to problems of recent general interest that were difficult or even impossible to achieve in the not so distant past. Integrating vibrational spectroscopy and computational approaches serves as a handbook for people performing vibrational spectroscopy followed by chemometric analysis hence both experimental methods as well as procedures of recommended analysis are described. This volume is written for individuals who develop new methodologies and extend these applications to new realms of chemical and medicinal interest.
Presents chemical state imaging methods useful on distance scales ranging from individual atoms to millimeters. This work is intended for chemists familiar with modern spectroscopies, but includes tutorial material on basic imaging processes for those with little background in the field.
Book Synopsis Microscopic and Spectroscopic Imaging of the Chemical State by : Michael D. Morris
Download or read book Microscopic and Spectroscopic Imaging of the Chemical State written by Michael D. Morris and published by CRC Press. This book was released on 1993-07-26 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents chemical state imaging methods useful on distance scales ranging from individual atoms to millimeters. This work is intended for chemists familiar with modern spectroscopies, but includes tutorial material on basic imaging processes for those with little background in the field.
Physical Techniques in Biological Research, Second Edition, Volume I, Part A: Optical Techniques focuses on the methods and theory applied to relatively pure preparations of biological substances that are derived from cells or other tissue elements. This book covers a wide variety of nonoptical techniques. Organized into five chapters, this edition begins with an overview of the fundamental principles of operation of optical instruments that can be explained in terms of the simple operations of lenses or light rays. This text then examines the limits imposed on direct observation, either through an optical instrument or with the unaided eye, by the nature of light itself. Other chapters consider the potential of certain other microprobe methods. This book discusses as well the process of molecular spectroscopy. The final chapter deals with optical rotation and its dependence on wavelength. This book is a valuable resource for biologists, scientists, physicists, physical chemists, and research workers.
Book Synopsis Optical Techniques by : Gerald Oster
Download or read book Optical Techniques written by Gerald Oster and published by Academic Press. This book was released on 2013-10-22 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physical Techniques in Biological Research, Second Edition, Volume I, Part A: Optical Techniques focuses on the methods and theory applied to relatively pure preparations of biological substances that are derived from cells or other tissue elements. This book covers a wide variety of nonoptical techniques. Organized into five chapters, this edition begins with an overview of the fundamental principles of operation of optical instruments that can be explained in terms of the simple operations of lenses or light rays. This text then examines the limits imposed on direct observation, either through an optical instrument or with the unaided eye, by the nature of light itself. Other chapters consider the potential of certain other microprobe methods. This book discusses as well the process of molecular spectroscopy. The final chapter deals with optical rotation and its dependence on wavelength. This book is a valuable resource for biologists, scientists, physicists, physical chemists, and research workers.
