Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region

Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region

Author: Duong Tan Nhut

Publisher: Springer Nature

Published: 2022-04-05

Total Pages: 400

ISBN-13: 9811664986

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This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems. This book will be of interest to teachers, researchers, scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.


Book Synopsis Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region by : Duong Tan Nhut

Download or read book Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region written by Duong Tan Nhut and published by Springer Nature. This book was released on 2022-04-05 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems. This book will be of interest to teachers, researchers, scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.

Advances in Plant Tissue Culture

Advances in Plant Tissue Culture

Author: Avinash Chandra Rai

Publisher: Academic Press

Published: 2022-05-28

Total Pages: 428

ISBN-13: 0323998194

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Advances in Plant Tissue Culture: Current Developments and Future Trends provides a complete and up-to-date text on all basic and applied aspects of plant tissue cultures and their latest application implications. It will be beneficial for students and early-career researchers of plant sciences and plant/agricultural biotechnology. Plant tissue culture has emerged as a sustainable way to meet the requirements of fresh produces, horticultural crops, medicinal or ornamental plants. Nowadays, plant tissue culture is an emerging filed applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry. This book covers the latest technology, broadly applied for crop improvement, clonal propagation, Somatic hybridization Embryo rescue, Germplasm conservation, genetic conservation, or for the preservation of endangered species. However, these technologies also play a vital role in breaking seed dormancy over conventional methods of conservation. Focuses on plant tissue culture as an emerging field applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry Includes current studies and innovations in biotechnology Covers commercialization and current perspectives in the field of plant tissue culture techniques


Book Synopsis Advances in Plant Tissue Culture by : Avinash Chandra Rai

Download or read book Advances in Plant Tissue Culture written by Avinash Chandra Rai and published by Academic Press. This book was released on 2022-05-28 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Plant Tissue Culture: Current Developments and Future Trends provides a complete and up-to-date text on all basic and applied aspects of plant tissue cultures and their latest application implications. It will be beneficial for students and early-career researchers of plant sciences and plant/agricultural biotechnology. Plant tissue culture has emerged as a sustainable way to meet the requirements of fresh produces, horticultural crops, medicinal or ornamental plants. Nowadays, plant tissue culture is an emerging filed applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry. This book covers the latest technology, broadly applied for crop improvement, clonal propagation, Somatic hybridization Embryo rescue, Germplasm conservation, genetic conservation, or for the preservation of endangered species. However, these technologies also play a vital role in breaking seed dormancy over conventional methods of conservation. Focuses on plant tissue culture as an emerging field applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry Includes current studies and innovations in biotechnology Covers commercialization and current perspectives in the field of plant tissue culture techniques

Tissue Culture Techniques for Horticultural Crops

Tissue Culture Techniques for Horticultural Crops

Author: Kenneth C. Torres

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 282

ISBN-13: 1461597560

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This book was written for those individuals who are concerned about the techniques and practices of plant cell cultures for horticultural crops. It was designed to serve as a text and reference for students and professionals in ornamental horticulture, fruit and vegetable crop pro duction, botany, forestry, and other areas of plant science. Research during the last twenty-five years in the area of plant tissue culture has led to many developments and changes in this field. Al though the techniques involved in the manipulation of plant tissue culture are now relatively straightforward, the presentation of these techniques in a short volume for the beginner in the field is generally unavailable. In addition to describing the techniques for establishment and manipulation of specific species, several chapters in this book also provide a brief, general review of important cultural parameters. Spe cific protocols and laboratory procedures may also be found in the appendix. I hope that this presentation of information will be helpful to those individuals wanting to apply plant tissue culture techniques for horticultural crops.


Book Synopsis Tissue Culture Techniques for Horticultural Crops by : Kenneth C. Torres

Download or read book Tissue Culture Techniques for Horticultural Crops written by Kenneth C. Torres and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was written for those individuals who are concerned about the techniques and practices of plant cell cultures for horticultural crops. It was designed to serve as a text and reference for students and professionals in ornamental horticulture, fruit and vegetable crop pro duction, botany, forestry, and other areas of plant science. Research during the last twenty-five years in the area of plant tissue culture has led to many developments and changes in this field. Al though the techniques involved in the manipulation of plant tissue culture are now relatively straightforward, the presentation of these techniques in a short volume for the beginner in the field is generally unavailable. In addition to describing the techniques for establishment and manipulation of specific species, several chapters in this book also provide a brief, general review of important cultural parameters. Spe cific protocols and laboratory procedures may also be found in the appendix. I hope that this presentation of information will be helpful to those individuals wanting to apply plant tissue culture techniques for horticultural crops.

Plant Tissue Culture: Propagation, Conservation and Crop Improvement

Plant Tissue Culture: Propagation, Conservation and Crop Improvement

Author: Mohammad Anis

Publisher: Springer

Published: 2016-10-08

Total Pages: 616

ISBN-13: 9811019177

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This book presents basic concepts, methodologies and applications of biotechnology for the conservation and propagation of aromatic, medicinal and other economic plants. It caters to the needs and challenges of researchers in plant biology, biotechnology, the medical sciences, pharmaceutical biotechnology and pharmacology areas by providing an accessible and cost-effective practical approach to micro-propagation and conservation strategies for plant species. It also includes illustrations describing a complete documentation of the results and research into particular plant species conducted by the authors over the past 5 years. Plant Biotechnology has been a subject of academic interest for a considerable time. In recent years, it has also become a useful tool in agriculture and medicine, as well as a popular area of biological research. Current economic growth is globally projected in a highly positive manner, but the challenges many countries face with regard to food, feed, malnutrition, infectious diseases, the newly identified life-style diseases, and energy shortages, all of which are worsened by an ever-deteriorating environment, continue to pull the growth digits back. The common thread that connects all of the above challenges is biotechnology, which could provide many answers. Molecular biology and biotechnology have now become an integral part of tissue culture research. The tremendous impact generated by genetic engineering and consequently of transgenics now allows us to manipulate plant genomes at will. There has indeed been a rapid development in this area with major successes in both developed and developing countries. The book introduces several new and exciting areas to researchers who are unfamiliar with plant biotechnology and also serves as a review of ongoing research and future directions for scholars. The book highlights numerous methods for in vitro propagation and utilization of techniques in raising transgenics to help readers reproduce the experiments discussed.


Book Synopsis Plant Tissue Culture: Propagation, Conservation and Crop Improvement by : Mohammad Anis

Download or read book Plant Tissue Culture: Propagation, Conservation and Crop Improvement written by Mohammad Anis and published by Springer. This book was released on 2016-10-08 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents basic concepts, methodologies and applications of biotechnology for the conservation and propagation of aromatic, medicinal and other economic plants. It caters to the needs and challenges of researchers in plant biology, biotechnology, the medical sciences, pharmaceutical biotechnology and pharmacology areas by providing an accessible and cost-effective practical approach to micro-propagation and conservation strategies for plant species. It also includes illustrations describing a complete documentation of the results and research into particular plant species conducted by the authors over the past 5 years. Plant Biotechnology has been a subject of academic interest for a considerable time. In recent years, it has also become a useful tool in agriculture and medicine, as well as a popular area of biological research. Current economic growth is globally projected in a highly positive manner, but the challenges many countries face with regard to food, feed, malnutrition, infectious diseases, the newly identified life-style diseases, and energy shortages, all of which are worsened by an ever-deteriorating environment, continue to pull the growth digits back. The common thread that connects all of the above challenges is biotechnology, which could provide many answers. Molecular biology and biotechnology have now become an integral part of tissue culture research. The tremendous impact generated by genetic engineering and consequently of transgenics now allows us to manipulate plant genomes at will. There has indeed been a rapid development in this area with major successes in both developed and developing countries. The book introduces several new and exciting areas to researchers who are unfamiliar with plant biotechnology and also serves as a review of ongoing research and future directions for scholars. The book highlights numerous methods for in vitro propagation and utilization of techniques in raising transgenics to help readers reproduce the experiments discussed.

Plant Tissue Culture

Plant Tissue Culture

Author: S.S. Bhojwani

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 470

ISBN-13: 0444598472

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During the past decade, Plant Tissue Culture (PTC) has attracted considerable attention because of its vital role in plant biotechnology. PTC offers novel approaches to plant production, propagation, and preservation. Some in vitro techniques are being applied on a commercial scale while many others hold great potential. Consequently, the literature in this area has grown rapidly.This book deals with recent developments in plant tissue culture, and presents a critical assessment of the proven and potential applications of the various in vitro techniques, it also highlights current problems limiting the application of tissue culture, and projects the future lines of research in this field.


Book Synopsis Plant Tissue Culture by : S.S. Bhojwani

Download or read book Plant Tissue Culture written by S.S. Bhojwani and published by Elsevier. This book was released on 2012-12-02 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decade, Plant Tissue Culture (PTC) has attracted considerable attention because of its vital role in plant biotechnology. PTC offers novel approaches to plant production, propagation, and preservation. Some in vitro techniques are being applied on a commercial scale while many others hold great potential. Consequently, the literature in this area has grown rapidly.This book deals with recent developments in plant tissue culture, and presents a critical assessment of the proven and potential applications of the various in vitro techniques, it also highlights current problems limiting the application of tissue culture, and projects the future lines of research in this field.

Plant Tissue Culture and Transformation Techniques

Plant Tissue Culture and Transformation Techniques

Author: Balram Sharma

Publisher: Daya Books

Published: 2008

Total Pages: 178

ISBN-13: 9788170355274

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This publication deals with various aspects of the genetic engineering-plant tissue culture and transformation techniques. Due to their biological, ecological and geographic diversity, the demand for various horticultural crops is likely to increase manifold in the future and in order to meet such demand, there is an urgent need to concentrate on the research aspects for improvement of these crops. Plant tissues culture offers new tools to accomplish this objective. Plant tissue culture is an important area of biotechnology, whic is used for the propagation of problem-species, rapid propagation of high value genotypes, production of secondary metabolites etc. Tissue culture is an important step in developing new hybrids from distant parents and transgenics and particularly cost-effective technology with palpable impact in vegetatively propagated plants, which is celarly visible in improved yields of cultivars incorporating genes from unexplored sources and improved germplasm, enhancement of quality parameters and supply of disease-free clones of true-to-type planting materials. Plant tissue culture is the most rapid and efficacious way to speedy productin of large volumes of identical plants for specific markets. Micropropagation is the quickest way for popularization of new varieties of horiticultural crops where other methods of mass multiplication of genetically pure and homogeneous planting materials are very slow. With the advent of transformation technology, it has become a useful tool to mass produce new plants with genetic material transferred from unrelated sources with the help of tissue culture. The volume contains contributions by several authors highlighting the status of genetic engineering and plant tissue culture research and development programmes in various developing countries and case studies on a few economically important crops. The publication will be of immense value to the working scientists, institutions, policy makers and all those bearing responsibility to develop, implement and intensify programmes in the related subjects in their respective countries. This book provides a good picture of efforts being made and success already achieved in the Third World countries at various levels of development striving to secure gains from the latest advances in science and technology. Contents Chapter 1: China-Cotton Genetic Engineering and Tissue Culture Developments by Reddy Naganagouda and Zhu Shuijin; Chapter 2: Egypt: Development of Transgenic Wheat with Improved Salt and Drought Tolerance by Ahmed Bahelidin & Hala F Eissa; Chapter 3: Egypt-Use of Genetic Engineering Approach to Develop Virus Resistance for Some Plants Belonging to Different Plant Families by Atef Shoukry Sadik; Chapter 4: Egypt-Genetic Transformation of Maize (Zea mays L) by Shireen Assem; Chapter 5: Egypt-Tissue Culture and Transformation of Potato by Taymour Nasr El Din; Chapter 6: Eritrea-Genetic Engineering by Tadesse Mehari; Chapter 7: India-Present Status, Policy and Constrains in Genetic Engineering by Jeetendra Jaysing Solanki; Chapter 8: Indonesia-Review on the Role of Biotechnology for Food Security by Lukit Devy; Chapter 9: Iran-Status of Agricultural Biotechnology by M Kafi; Chapter 10: Kenya-Status of Biotechnology Research and Development by C N Ngaman, M G Karembu and D Otunge; Chapter 11: Kenya-Present Status, Policies and Constraints in Areas Related to Plant Biotechnology by Salome Mallowa Obura; Chapter 12: Malaysia-A Brief Report on Biotechnology and Genetic Engineering by Z A Aziz; Chapter 13: Pakistan-Present Status, Policies and Constraints of Biotechnology by Saghir Ahmed Sheikh; Chapter 14: Sri Lanks-Present Status of Biotechnology by P Aruni Weerasinghe; Chapter 15: Syria-Current Status and Future Prospective of Agricultural Biotechnology Program at GCSAR by Nabila Ali Bacha; Chapter 16: Uganda-Report on the Present Status Policies and Constraints to Genetic Engineering by Kyeyune Gerald Muwanga.


Book Synopsis Plant Tissue Culture and Transformation Techniques by : Balram Sharma

Download or read book Plant Tissue Culture and Transformation Techniques written by Balram Sharma and published by Daya Books. This book was released on 2008 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: This publication deals with various aspects of the genetic engineering-plant tissue culture and transformation techniques. Due to their biological, ecological and geographic diversity, the demand for various horticultural crops is likely to increase manifold in the future and in order to meet such demand, there is an urgent need to concentrate on the research aspects for improvement of these crops. Plant tissues culture offers new tools to accomplish this objective. Plant tissue culture is an important area of biotechnology, whic is used for the propagation of problem-species, rapid propagation of high value genotypes, production of secondary metabolites etc. Tissue culture is an important step in developing new hybrids from distant parents and transgenics and particularly cost-effective technology with palpable impact in vegetatively propagated plants, which is celarly visible in improved yields of cultivars incorporating genes from unexplored sources and improved germplasm, enhancement of quality parameters and supply of disease-free clones of true-to-type planting materials. Plant tissue culture is the most rapid and efficacious way to speedy productin of large volumes of identical plants for specific markets. Micropropagation is the quickest way for popularization of new varieties of horiticultural crops where other methods of mass multiplication of genetically pure and homogeneous planting materials are very slow. With the advent of transformation technology, it has become a useful tool to mass produce new plants with genetic material transferred from unrelated sources with the help of tissue culture. The volume contains contributions by several authors highlighting the status of genetic engineering and plant tissue culture research and development programmes in various developing countries and case studies on a few economically important crops. The publication will be of immense value to the working scientists, institutions, policy makers and all those bearing responsibility to develop, implement and intensify programmes in the related subjects in their respective countries. This book provides a good picture of efforts being made and success already achieved in the Third World countries at various levels of development striving to secure gains from the latest advances in science and technology. Contents Chapter 1: China-Cotton Genetic Engineering and Tissue Culture Developments by Reddy Naganagouda and Zhu Shuijin; Chapter 2: Egypt: Development of Transgenic Wheat with Improved Salt and Drought Tolerance by Ahmed Bahelidin & Hala F Eissa; Chapter 3: Egypt-Use of Genetic Engineering Approach to Develop Virus Resistance for Some Plants Belonging to Different Plant Families by Atef Shoukry Sadik; Chapter 4: Egypt-Genetic Transformation of Maize (Zea mays L) by Shireen Assem; Chapter 5: Egypt-Tissue Culture and Transformation of Potato by Taymour Nasr El Din; Chapter 6: Eritrea-Genetic Engineering by Tadesse Mehari; Chapter 7: India-Present Status, Policy and Constrains in Genetic Engineering by Jeetendra Jaysing Solanki; Chapter 8: Indonesia-Review on the Role of Biotechnology for Food Security by Lukit Devy; Chapter 9: Iran-Status of Agricultural Biotechnology by M Kafi; Chapter 10: Kenya-Status of Biotechnology Research and Development by C N Ngaman, M G Karembu and D Otunge; Chapter 11: Kenya-Present Status, Policies and Constraints in Areas Related to Plant Biotechnology by Salome Mallowa Obura; Chapter 12: Malaysia-A Brief Report on Biotechnology and Genetic Engineering by Z A Aziz; Chapter 13: Pakistan-Present Status, Policies and Constraints of Biotechnology by Saghir Ahmed Sheikh; Chapter 14: Sri Lanks-Present Status of Biotechnology by P Aruni Weerasinghe; Chapter 15: Syria-Current Status and Future Prospective of Agricultural Biotechnology Program at GCSAR by Nabila Ali Bacha; Chapter 16: Uganda-Report on the Present Status Policies and Constraints to Genetic Engineering by Kyeyune Gerald Muwanga.

Introduction to Plant Tissue Culture

Introduction to Plant Tissue Culture

Author: M. K. Razdan

Publisher: Science Publishers

Published: 2003

Total Pages: 394

ISBN-13: 9781578082377

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Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.


Book Synopsis Introduction to Plant Tissue Culture by : M. K. Razdan

Download or read book Introduction to Plant Tissue Culture written by M. K. Razdan and published by Science Publishers. This book was released on 2003 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.

Plant Tissue Culture

Plant Tissue Culture

Author: Sunghun Park

Publisher: Academic Press

Published: 2021-02-17

Total Pages: 254

ISBN-13: 0323851371

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Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists. Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Contains the latest advances and updates since the previous edition published in 2012


Book Synopsis Plant Tissue Culture by : Sunghun Park

Download or read book Plant Tissue Culture written by Sunghun Park and published by Academic Press. This book was released on 2021-02-17 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists. Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Contains the latest advances and updates since the previous edition published in 2012

Plant Tissue Culture: An Introductory Text

Plant Tissue Culture: An Introductory Text

Author: Sant Saran Bhojwani

Publisher: Springer Science & Business Media

Published: 2013-03-20

Total Pages: 318

ISBN-13: 8132210263

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Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book.


Book Synopsis Plant Tissue Culture: An Introductory Text by : Sant Saran Bhojwani

Download or read book Plant Tissue Culture: An Introductory Text written by Sant Saran Bhojwani and published by Springer Science & Business Media. This book was released on 2013-03-20 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book.

Plant Tissue Culture

Plant Tissue Culture

Author: Roberta H. Smith

Publisher: Academic Press

Published: 2013

Total Pages: 206

ISBN-13: 0124159206

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Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature


Book Synopsis Plant Tissue Culture by : Roberta H. Smith

Download or read book Plant Tissue Culture written by Roberta H. Smith and published by Academic Press. This book was released on 2013 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature