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Ultrafiltration is a pressure-driven, membrane-based separation process, which is used for a broad variety of applications, ranging from the processing of biological macromolecules to wastewater treatment. It has significant advantages over competing separation technologies. Food and biotechnological applications account for nearly 40% of the current total usage of ultrafiltration membranes. Protein bioseparation is an important component of this application segment. Ultrafiltration is used for protein concentration, desalting, clarification and fractionation (i.e. protein-protein separation). Concentration, desalting and clarification are technologically less demanding and have been in used in the bioprocess industry for some time. Protein fractionation, on the other hand, is a challenging proposition and is definitely a more recent development. This book focuses primarily on protein fractionation./a
Book Synopsis Protein Bioseparation Using Ultrafiltration: Theory, Applications And New Developments by : Raja Ghosh
Download or read book Protein Bioseparation Using Ultrafiltration: Theory, Applications And New Developments written by Raja Ghosh and published by World Scientific. This book was released on 2003-06-11 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ultrafiltration is a pressure-driven, membrane-based separation process, which is used for a broad variety of applications, ranging from the processing of biological macromolecules to wastewater treatment. It has significant advantages over competing separation technologies. Food and biotechnological applications account for nearly 40% of the current total usage of ultrafiltration membranes. Protein bioseparation is an important component of this application segment. Ultrafiltration is used for protein concentration, desalting, clarification and fractionation (i.e. protein-protein separation). Concentration, desalting and clarification are technologically less demanding and have been in used in the bioprocess industry for some time. Protein fractionation, on the other hand, is a challenging proposition and is definitely a more recent development. This book focuses primarily on protein fractionation./a
Protein bioseparation is an important yet expensive activity especially in biotechnology industry. The production of protein is growth along with rapid commercialization and demand. Unfortunately, the industry having problem in term of cost and the production as the cost is extremely high but the production rate is low. The most effective way to solve this problem is by using membrane as the medium of separation. In this study, the effects for different mode of operations of ultrafiltration membranes - batch, single stage and two stages forward cascade. Amersham Quick Lab cross flow system was used to separate lysozyme by using 30 kDa polyethersulfone ultrafiltration membranes. For this research, the lysozyme is obtained from the cheapest source which is chicken egg white solution. Lysozyme is a very important protein in pharmaceutical field in order to produce a vaccine to prevent bacteria that inhabit the intestinal tract. In order to determine the most efficient configurations, the constant parameters are set for all configurations. The value of pH must be 10, the transmembrane pressure (TMP) is 1.0 bar, and the volume feed is 960 ml with the ratio of chicken egg white to sodium chloride 0.2 M is 1:31. The effective configuration can be determined by comparing the permeate flux, concentration of lysozyme in both permeate and retentate solution at 5 minutes after the separation process occurred and measurement of water flux. The analysis procedure is done using the UV-VIS spectrometer at wave length equal to 595 nm. The most effective configuration is two stages forward cascade which has the highest average permeate flux of 74.4 L/m2.h. Moreover, it also has percentage for concentration of lysozyme in permeate solution equal to 84% which is the biggest percentage of all configurations and the lowest value of water flux measurement after separation process compare to the benchmark value at TMP equal to 1.0 bar. The second configuration then is single stage and followed by batch at third place.
Book Synopsis Protein Fractionation Using Ultrafiltration System for Differents Module Configurations by : Emy Syafinas Hamid @ Osman
Download or read book Protein Fractionation Using Ultrafiltration System for Differents Module Configurations written by Emy Syafinas Hamid @ Osman and published by . This book was released on 2006 with total page 71 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein bioseparation is an important yet expensive activity especially in biotechnology industry. The production of protein is growth along with rapid commercialization and demand. Unfortunately, the industry having problem in term of cost and the production as the cost is extremely high but the production rate is low. The most effective way to solve this problem is by using membrane as the medium of separation. In this study, the effects for different mode of operations of ultrafiltration membranes - batch, single stage and two stages forward cascade. Amersham Quick Lab cross flow system was used to separate lysozyme by using 30 kDa polyethersulfone ultrafiltration membranes. For this research, the lysozyme is obtained from the cheapest source which is chicken egg white solution. Lysozyme is a very important protein in pharmaceutical field in order to produce a vaccine to prevent bacteria that inhabit the intestinal tract. In order to determine the most efficient configurations, the constant parameters are set for all configurations. The value of pH must be 10, the transmembrane pressure (TMP) is 1.0 bar, and the volume feed is 960 ml with the ratio of chicken egg white to sodium chloride 0.2 M is 1:31. The effective configuration can be determined by comparing the permeate flux, concentration of lysozyme in both permeate and retentate solution at 5 minutes after the separation process occurred and measurement of water flux. The analysis procedure is done using the UV-VIS spectrometer at wave length equal to 595 nm. The most effective configuration is two stages forward cascade which has the highest average permeate flux of 74.4 L/m2.h. Moreover, it also has percentage for concentration of lysozyme in permeate solution equal to 84% which is the biggest percentage of all configurations and the lowest value of water flux measurement after separation process compare to the benchmark value at TMP equal to 1.0 bar. The second configuration then is single stage and followed by batch at third place.
There is a critical need to develop new technologies that provide high resolution protein purification at a price, scale, and throughput needed for the production of high value protein products. Affinity chromatography can provide very high selectivity, but throughput is often reduced by mass transfer limitations. Affinity ultrafiltration is a potentially attractive alternative, but the high cost and practical limitations of the large macroligands used in previous studies has limited the viability of this technique. The overall objective of this work was to demonstrate the feasibility of affinity ultrafiltration using small charged affinity ligands, with the change in protein charge exploited for high resolution separation using an electrically charged membrane.
Book Synopsis Protein Separation Using Affinity Ultrafiltration with Small Charged Ligands by : Suma Rao
Download or read book Protein Separation Using Affinity Ultrafiltration with Small Charged Ligands written by Suma Rao and published by . This book was released on 2006 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is a critical need to develop new technologies that provide high resolution protein purification at a price, scale, and throughput needed for the production of high value protein products. Affinity chromatography can provide very high selectivity, but throughput is often reduced by mass transfer limitations. Affinity ultrafiltration is a potentially attractive alternative, but the high cost and practical limitations of the large macroligands used in previous studies has limited the viability of this technique. The overall objective of this work was to demonstrate the feasibility of affinity ultrafiltration using small charged affinity ligands, with the change in protein charge exploited for high resolution separation using an electrically charged membrane.
Book Synopsis Protein Separation by Electro-ultrafiltration by : Todd Anthony Church
Download or read book Protein Separation by Electro-ultrafiltration written by Todd Anthony Church and published by . This book was released on 1994 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Offers in-depth coverage of the latest advances in new and traditional separation technologies as they are used in a variety of ways to produce value-added products. Examines both fundamental and applied aspects of separation techniques.
Book Synopsis Bioseparation Processes in Food by : Singh
Download or read book Bioseparation Processes in Food written by Singh and published by CRC Press. This book was released on 1995-05-16 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers in-depth coverage of the latest advances in new and traditional separation technologies as they are used in a variety of ways to produce value-added products. Examines both fundamental and applied aspects of separation techniques.
Book Synopsis Highly Selective Membranes in Protein Ultrafiltration by : Meredith Feins
Download or read book Highly Selective Membranes in Protein Ultrafiltration written by Meredith Feins and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein Bioseparation Using Synthetic Membranes by : Dharmeshkumar M. Kanani
Download or read book Protein Bioseparation Using Synthetic Membranes written by Dharmeshkumar M. Kanani and published by . This book was released on 2007 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt:
The biopharmaceutical industry has become an increasingly important player in the global economy, and the success of these products depends on the development and implementation of cost-effective, robust and scaleable production processes. Bioseparations-also called downstream processing- can be a key source of competitive advantageto biopharmaceut
Book Synopsis Process Scale Bioseparations for the Biopharmaceutical Industry by : Abhinav A. Shukla
Download or read book Process Scale Bioseparations for the Biopharmaceutical Industry written by Abhinav A. Shukla and published by CRC Press. This book was released on 2006-07-07 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: The biopharmaceutical industry has become an increasingly important player in the global economy, and the success of these products depends on the development and implementation of cost-effective, robust and scaleable production processes. Bioseparations-also called downstream processing- can be a key source of competitive advantageto biopharmaceut
The Handbook of Membrane Separations: Chemical, Pharmaceutical, and Biotechnological Applications provides detailed information on membrane separation technologies as they have evolved over the past decades. To provide a basic understanding of membrane technology, this book documents the developments dealing with these technologies. It explores chemical, pharmaceutical, food processing and biotechnological applications of membrane processes ranging from selective separation to solvent and material recovery. This text also presents in-depth knowledge of membrane separation mechanisms, transport models, membrane permeability computations, membrane types and modules, as well as membrane reactors.
Book Synopsis Handbook of Membrane Separations by : Anil K. Pabby
Download or read book Handbook of Membrane Separations written by Anil K. Pabby and published by CRC Press. This book was released on 2008-07-07 with total page 1210 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Membrane Separations: Chemical, Pharmaceutical, and Biotechnological Applications provides detailed information on membrane separation technologies as they have evolved over the past decades. To provide a basic understanding of membrane technology, this book documents the developments dealing with these technologies. It explores chemical, pharmaceutical, food processing and biotechnological applications of membrane processes ranging from selective separation to solvent and material recovery. This text also presents in-depth knowledge of membrane separation mechanisms, transport models, membrane permeability computations, membrane types and modules, as well as membrane reactors.
This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation. Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations. Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures. Key Features * Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include: * detailed data on biological stability under various bioseparation conditions * extensive case studies from the literature on separation processes, validation, and economics * simplified analysis of protein refolding and inactivation mechanisms * consideration of adsorption theories and the effect of heterogeneity * coverage of both classical and novel bioseparation techniques, including chromatographic procedures
Book Synopsis Bioseparations of Proteins by : Ajit Sadana
Download or read book Bioseparations of Proteins written by Ajit Sadana and published by Elsevier. This book was released on 1997-11-27 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation. Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations. Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures. Key Features * Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include: * detailed data on biological stability under various bioseparation conditions * extensive case studies from the literature on separation processes, validation, and economics * simplified analysis of protein refolding and inactivation mechanisms * consideration of adsorption theories and the effect of heterogeneity * coverage of both classical and novel bioseparation techniques, including chromatographic procedures
