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Recombinant DNA Methodology

Author:

Publisher: Academic Press

Published: 2014-05-19

Total Pages: 793

ISBN-13: 1483295974

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Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry. Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher. Enzymes in DNA research DNA isolation, hybridization, and cloning DNA sequence analysis cDNA cloning Gene products Identification of cloned genes and mapping of genes Monitoring cloned gene expression Cloning and transferring of genes into yeast cells Cloning and transferring of genes into plant cells Cloning and transferring of genes into animal cells Site-directed mutagenesis Protein engineering Expression vectors

Book Synopsis Recombinant DNA Methodology by :

Download or read book Recombinant DNA Methodology written by and published by Academic Press. This book was released on 2014-05-19 with total page 793 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry. Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher. Enzymes in DNA research DNA isolation, hybridization, and cloning DNA sequence analysis cDNA cloning Gene products Identification of cloned genes and mapping of genes Monitoring cloned gene expression Cloning and transferring of genes into yeast cells Cloning and transferring of genes into plant cells Cloning and transferring of genes into animal cells Site-directed mutagenesis Protein engineering Expression vectors

Recombinant DNA Methodology

Author: Ray Wu

Publisher: Academic Press

Published: 1989-06-28

Total Pages: 800

ISBN-13:

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Enzymes in recombinant DNA research. DNA-joining enzymes: a review. Guide to the use of type II restriction endonucleases. Site-specific cleavage of DNA at 8-,9-, and 10-bp sequences. Exonuclease III: use for DNA sequence analysis and in specific deletions of nucleotides. Methods for isolation, purification, or amplification of DNA. Gel electrophoresis of restriction fragments. Purification, specific fragmentation, and separation of large DNA molecules. Orthogonal-field-alternation gel electrophoresis. A rapid alkaline extraction method for the isolation of plasmid DNA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Vectors or methods for gene cloning. Escherichia coli plasmids packageable in vitro in a bacteriophage particles. Production of single-stranded plasmid DNA. High-efficiency cloning of full-length cDNA: construction and screening of cDNA expression libraries for mannalian cells. Transformation and preservation of competent bacterial cells by freezing. Plasmid screening high colony density. New bacteriophage lambda vectors with positive selection for cloned inserts. A phage vectors-EMBL series. Agt 11: gene isolation with antibody probes and other applications. One-step gene disruption in yeast. Cloning regulated yeast genes from a pool of lacZ fusions. Selection procedure for isolation fo centromere DNAs from rom saccharomyces cerevisiae. Construction of high copy yeast vectors using 2-um circle sequences. Improved vectors for plant transformation: expression cassette ...

Book Synopsis Recombinant DNA Methodology by : Ray Wu

Download or read book Recombinant DNA Methodology written by Ray Wu and published by Academic Press. This book was released on 1989-06-28 with total page 800 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes in recombinant DNA research. DNA-joining enzymes: a review. Guide to the use of type II restriction endonucleases. Site-specific cleavage of DNA at 8-,9-, and 10-bp sequences. Exonuclease III: use for DNA sequence analysis and in specific deletions of nucleotides. Methods for isolation, purification, or amplification of DNA. Gel electrophoresis of restriction fragments. Purification, specific fragmentation, and separation of large DNA molecules. Orthogonal-field-alternation gel electrophoresis. A rapid alkaline extraction method for the isolation of plasmid DNA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Vectors or methods for gene cloning. Escherichia coli plasmids packageable in vitro in a bacteriophage particles. Production of single-stranded plasmid DNA. High-efficiency cloning of full-length cDNA: construction and screening of cDNA expression libraries for mannalian cells. Transformation and preservation of competent bacterial cells by freezing. Plasmid screening high colony density. New bacteriophage lambda vectors with positive selection for cloned inserts. A phage vectors-EMBL series. Agt 11: gene isolation with antibody probes and other applications. One-step gene disruption in yeast. Cloning regulated yeast genes from a pool of lacZ fusions. Selection procedure for isolation fo centromere DNAs from rom saccharomyces cerevisiae. Construction of high copy yeast vectors using 2-um circle sequences. Improved vectors for plant transformation: expression cassette ...

Enzymology Primer for Recombinant DNA Technology

Author: Hyone-Myong Eun

Publisher: Elsevier

Published: 1996-06-03

Total Pages: 727

ISBN-13: 008053113X

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Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology Presents information in an easily accessible format to serve as a quick reference source Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques

Book Synopsis Enzymology Primer for Recombinant DNA Technology by : Hyone-Myong Eun

Download or read book Enzymology Primer for Recombinant DNA Technology written by Hyone-Myong Eun and published by Elsevier. This book was released on 1996-06-03 with total page 727 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology Presents information in an easily accessible format to serve as a quick reference source Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques

Recombinant DNA Methodology II

Author: Ray Wu

Publisher:

Published: 1995

Total Pages: 948

ISBN-13:

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Book Synopsis Recombinant DNA Methodology II by : Ray Wu

Download or read book Recombinant DNA Methodology II written by Ray Wu and published by . This book was released on 1995 with total page 948 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Calculations for Molecular Biology and Biotechnology

Author: Frank H. Stephenson

Publisher: Academic Press

Published: 2010-07-30

Total Pages: 460

ISBN-13: 9780123756916

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Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: Updated and increased coverage of real time PCR and the mathematics used to measure gene expression More sample problems in every chapter for readers to practice concepts

Book Synopsis Calculations for Molecular Biology and Biotechnology by : Frank H. Stephenson

Download or read book Calculations for Molecular Biology and Biotechnology written by Frank H. Stephenson and published by Academic Press. This book was released on 2010-07-30 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: Updated and increased coverage of real time PCR and the mathematics used to measure gene expression More sample problems in every chapter for readers to practice concepts

Recombinant DNA Principles and Methodologies

Author: James Greene

Publisher: CRC Press

Published: 2021-12-17

Total Pages: 768

ISBN-13: 1000105474

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This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

Book Synopsis Recombinant DNA Principles and Methodologies by : James Greene

Download or read book Recombinant DNA Principles and Methodologies written by James Greene and published by CRC Press. This book was released on 2021-12-17 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

Recombinant DNA Methodology II

Author: Ray Wu

Publisher: Academic Press

Published: 2012-12-02

Total Pages: 935

ISBN-13: 0323137741

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The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. * Methods for: * DNA isolation and cloning* Synthesizing complementary DNA (cDNA)* Cleaving and manipulating DNA * Selecting useful reporter genes* Constructing vectors for cloning genes* Constructing expression vectors* Site-directed mutagenesis and gene disruption* Identifying and mapping genes* Transforming animal and plant cells* Sequencing DNA* Amplifying and manipulating DNA and PCR* Detecting DNA - protein interaction

Book Synopsis Recombinant DNA Methodology II by : Ray Wu

Download or read book Recombinant DNA Methodology II written by Ray Wu and published by Academic Press. This book was released on 2012-12-02 with total page 935 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. * Methods for: * DNA isolation and cloning* Synthesizing complementary DNA (cDNA)* Cleaving and manipulating DNA * Selecting useful reporter genes* Constructing vectors for cloning genes* Constructing expression vectors* Site-directed mutagenesis and gene disruption* Identifying and mapping genes* Transforming animal and plant cells* Sequencing DNA* Amplifying and manipulating DNA and PCR* Detecting DNA - protein interaction

DNA Recombination and Repair

Author: Paul James Smith

Publisher: Oxford University Press, USA

Published: 1999

Total Pages: 260

ISBN-13:

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The processes of DNA recombination and repair are vital to cell integrity - an error can lead to disease such as cancer. It is therefore a large and exciting area of research and is also taught on postgraduate and undergraduate courses. This book is not a comprehensive view of the field, but a selection of the issues currently at the forefront of knowledge.

Book Synopsis DNA Recombination and Repair by : Paul James Smith

Download or read book DNA Recombination and Repair written by Paul James Smith and published by Oxford University Press, USA. This book was released on 1999 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: The processes of DNA recombination and repair are vital to cell integrity - an error can lead to disease such as cancer. It is therefore a large and exciting area of research and is also taught on postgraduate and undergraduate courses. This book is not a comprehensive view of the field, but a selection of the issues currently at the forefront of knowledge.

Recombinant DNA Technology

Author: Keya Chaudhuri

Publisher: The Energy and Resources Institute (TERI)

Published: 2013-01-01

Total Pages: 300

ISBN-13: 8179933202

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Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences.

Book Synopsis Recombinant DNA Technology by : Keya Chaudhuri

Download or read book Recombinant DNA Technology written by Keya Chaudhuri and published by The Energy and Resources Institute (TERI). This book was released on 2013-01-01 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences.

Molecular Biotechnology

Author: Bernard R. Glick

Publisher:

Published: 1998

Total Pages: 724

ISBN-13:

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The second edition explains the principles of recombinant DNA technology as well as other important techniques such as DNA sequencing, the polymerase chain reaction, and the production of monclonal antibodies.

Book Synopsis Molecular Biotechnology by : Bernard R. Glick

Download or read book Molecular Biotechnology written by Bernard R. Glick and published by . This book was released on 1998 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition explains the principles of recombinant DNA technology as well as other important techniques such as DNA sequencing, the polymerase chain reaction, and the production of monclonal antibodies.