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Pocket-sized text provides the procedures for taking accurate vital signs. Provides an historical overview and covers such vital signs as temperature, heart rate, respiration, blood pressure, and level of consciousness. For nurses, residents and physicians. Wire-spiral binding.
Book Synopsis Recombinant Protease Inhibitors in Plants by : Dominique Michaud
Download or read book Recombinant Protease Inhibitors in Plants written by Dominique Michaud and published by CRC Press. This book was released on 2001-07-01 with total page 209 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pocket-sized text provides the procedures for taking accurate vital signs. Provides an historical overview and covers such vital signs as temperature, heart rate, respiration, blood pressure, and level of consciousness. For nurses, residents and physicians. Wire-spiral binding.
Table of Contents: 1. Protease/inhibitor interactions in plant-pest systems: a brief overview, 2. Control of Phytophagous insect pests using serine protease inhibitors, 3. Cystatin-based control of insects, with special erference to Oryzacystatin.
Book Synopsis Recombinant Protease Inhibitors in Plants by :
Download or read book Recombinant Protease Inhibitors in Plants written by and published by . This book was released on 2000 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: Table of Contents: 1. Protease/inhibitor interactions in plant-pest systems: a brief overview, 2. Control of Phytophagous insect pests using serine protease inhibitors, 3. Cystatin-based control of insects, with special erference to Oryzacystatin.
Plant protease inhibitors are diverse in number & specificity towards various proteolytic enzymes.
Book Synopsis Plant Protease Inhibitors by : Yehudith Birk
Download or read book Plant Protease Inhibitors written by Yehudith Birk and published by Springer Science & Business Media. This book was released on 2003-04-08 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant protease inhibitors are diverse in number & specificity towards various proteolytic enzymes.
Plant proteases are involved in most aspects of plant physiology and development, playing key roles in the generation of signaling molecules and as regulators of essential cellular processes such as cell division and metabolism. They take part in important pathways like protein turnover by the degradation of misfolded proteins and the ubiquitin-proteasome pathway, and they are responsible for post-translational modifications of proteins by proteolysis at highly specific sites. Proteases are also implicated in a great variety of environmentally controlled processes, including mobilization of storage proteins during seed germination, development of seedlings, senescence, programmed cell death and defense mechanisms against pests and pathogens. However, in spite of their importance, little is known about the functions and mode of actions of specific plant proteases. This Research Topic collects contributions covering diverse aspects of plant proteases research.
Book Synopsis Plant Proteases by : Mercedes Diaz-Mendoza
Download or read book Plant Proteases written by Mercedes Diaz-Mendoza and published by Frontiers Media SA. This book was released on 2020-01-24 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant proteases are involved in most aspects of plant physiology and development, playing key roles in the generation of signaling molecules and as regulators of essential cellular processes such as cell division and metabolism. They take part in important pathways like protein turnover by the degradation of misfolded proteins and the ubiquitin-proteasome pathway, and they are responsible for post-translational modifications of proteins by proteolysis at highly specific sites. Proteases are also implicated in a great variety of environmentally controlled processes, including mobilization of storage proteins during seed germination, development of seedlings, senescence, programmed cell death and defense mechanisms against pests and pathogens. However, in spite of their importance, little is known about the functions and mode of actions of specific plant proteases. This Research Topic collects contributions covering diverse aspects of plant proteases research.
Scientific Study from the year 2011 in the subject Biology - Human Biology, , course: Post Graduate Doctoral Studies, language: English, abstract: This book chapter covers the previous and current research on plant protease inhibitors being conducted worldwide since its identification. Plant protease inhibitors exists as natural defense mechanism in plants. Solanaceous plants have highly been explored for the protease inhibitor expression and function against various insects of the order lepidoptera, coleoptera, diptera and many more. Plant protease inhibitors are the well studied class of plant defensive proteins. High level of up-regulation upon insect damage, significantly elevated levels in reproductive and storage organs as well as specificity to pest proteases ascertain their defensive function. In the present chapter, we introduced molecular basis of plant-insect interactions and focused on different classes of protease inhibitors based on their target protease(s).
Book Synopsis Protease Inhibitors: Potential and Constraints by : Neha Khandelwal
Download or read book Protease Inhibitors: Potential and Constraints written by Neha Khandelwal and published by GRIN Verlag. This book was released on 2013-02-26 with total page 49 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific Study from the year 2011 in the subject Biology - Human Biology, , course: Post Graduate Doctoral Studies, language: English, abstract: This book chapter covers the previous and current research on plant protease inhibitors being conducted worldwide since its identification. Plant protease inhibitors exists as natural defense mechanism in plants. Solanaceous plants have highly been explored for the protease inhibitor expression and function against various insects of the order lepidoptera, coleoptera, diptera and many more. Plant protease inhibitors are the well studied class of plant defensive proteins. High level of up-regulation upon insect damage, significantly elevated levels in reproductive and storage organs as well as specificity to pest proteases ascertain their defensive function. In the present chapter, we introduced molecular basis of plant-insect interactions and focused on different classes of protease inhibitors based on their target protease(s).
Abstract: The interplay between proteases and protease inhibitors during plant-pathogen interaction represents a common strategy for defense and counter-defense. The plant pathogens Phytophthora infestans and Phytophthora mirabilis secrete effectors such as protease inhibitors that facilitate host colonization through a defense-counterdefense mechanism. The P. infestans serine protease inhibitors EPI1 and EPI10 physically bind and inhibit the tomato serine protease P69B. On the other hand, the P. infestans cysteine protease inhibitor EPIC2B targets PIP1, a papain-like protease that has close similarity to another tomato cysteine protease Rcr3, which is required for the fungal resistance and Avr2 hypersensitivity in Cf-2 tomato. The objective of this research is to characterize these protease inhibitors and their association with specific targets in the host. We studied the structure and activities of these protease inhibitors and their target proteases using recombinant proteins expressed in Escherichia coli and Nicotiana benthamiana. PIP1-His was pulled down using coimmunoprecipitation with anti-FLAG resin from N. benthamiana apoplast by recombinant protein FLAG-EPIC2B, suggesting physical interaction of EPIC2B and PIP1. Similarly, tomato protease Rcr3pim was shown to be a common target for both the Cladosporium fulvum effector Avr2 and P. infestans effector EPIC2B using pull down assays and DCG-04 activity profiling. However, unlike Avr2, EPIC2B is a reversible inhibitor of Rcr3pim and does not trigger hypersensitivity on Cf-2/Rcr3pim tomato. We also found that the rcr3-3 mutant of tomato that carries a premature stop codon in the Rcr3 gene exhibits enhanced susceptibility to P. infestans, suggesting a role for Rcr3pim in basal defense. It appears that relative to C. fulvum, P. infestans evolved a cunning effector that carries virulence activity without triggering plant innate immunity. Like EPIC1, PmEPIC1, an EPIC1 homolog from P. mirabilis, was also found to bind Rcr3pim but not PIP1. Unlike EPIC2B, neither EPIC1 nor PmEPIC1 binds or inhibits PIP1. This work was possible by the ability to express and purify proteins in the apoplast of N. benthamiana. Three tomato proteases with C-terminal 6XHistidine tag were successfully expressed in N. benthamiana apoplast. Our findings suggest that C-terminal His-tagging of proteins in N. benthamiana apoplast is efficient enough to enable purification of functional proteins.
Book Synopsis Functional Characterization of Extracellular Protease Inhibitors of Phytophthora Spp and Their Targets Tomato Proteases by : Jing Song
Download or read book Functional Characterization of Extracellular Protease Inhibitors of Phytophthora Spp and Their Targets Tomato Proteases written by Jing Song and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: The interplay between proteases and protease inhibitors during plant-pathogen interaction represents a common strategy for defense and counter-defense. The plant pathogens Phytophthora infestans and Phytophthora mirabilis secrete effectors such as protease inhibitors that facilitate host colonization through a defense-counterdefense mechanism. The P. infestans serine protease inhibitors EPI1 and EPI10 physically bind and inhibit the tomato serine protease P69B. On the other hand, the P. infestans cysteine protease inhibitor EPIC2B targets PIP1, a papain-like protease that has close similarity to another tomato cysteine protease Rcr3, which is required for the fungal resistance and Avr2 hypersensitivity in Cf-2 tomato. The objective of this research is to characterize these protease inhibitors and their association with specific targets in the host. We studied the structure and activities of these protease inhibitors and their target proteases using recombinant proteins expressed in Escherichia coli and Nicotiana benthamiana. PIP1-His was pulled down using coimmunoprecipitation with anti-FLAG resin from N. benthamiana apoplast by recombinant protein FLAG-EPIC2B, suggesting physical interaction of EPIC2B and PIP1. Similarly, tomato protease Rcr3pim was shown to be a common target for both the Cladosporium fulvum effector Avr2 and P. infestans effector EPIC2B using pull down assays and DCG-04 activity profiling. However, unlike Avr2, EPIC2B is a reversible inhibitor of Rcr3pim and does not trigger hypersensitivity on Cf-2/Rcr3pim tomato. We also found that the rcr3-3 mutant of tomato that carries a premature stop codon in the Rcr3 gene exhibits enhanced susceptibility to P. infestans, suggesting a role for Rcr3pim in basal defense. It appears that relative to C. fulvum, P. infestans evolved a cunning effector that carries virulence activity without triggering plant innate immunity. Like EPIC1, PmEPIC1, an EPIC1 homolog from P. mirabilis, was also found to bind Rcr3pim but not PIP1. Unlike EPIC2B, neither EPIC1 nor PmEPIC1 binds or inhibits PIP1. This work was possible by the ability to express and purify proteins in the apoplast of N. benthamiana. Three tomato proteases with C-terminal 6XHistidine tag were successfully expressed in N. benthamiana apoplast. Our findings suggest that C-terminal His-tagging of proteins in N. benthamiana apoplast is efficient enough to enable purification of functional proteins.
Scientific Study from the year 2011 in the subject Biology - Human Biology, grade: -, - (National Chemical Laboratory, Pune), course: Post Graduate Doctoral Studies, language: English, abstract: This book chapter covers the previous and current research on plant protease inhibitors being conducted worldwide since its identification. Plant protease inhibitors exists as natural defense mechanism in plants. Solanaceous plants have highly been explored for the protease inhibitor expression and function against various insects of the order lepidoptera, coleoptera, diptera and many more. Plant protease inhibitors are the well studied class of plant defensive proteins. High level of up-regulation upon insect damage, significantly elevated levels in reproductive and storage organs as well as specificity to pest proteases ascertain their defensive function. In the present chapter, we introduced molecular basis of plant-insect interactions and focused on different classes of protease inhibitors based on their target protease(s).
Book Synopsis Protease Inhibitors by : Neha Khandelwal
Download or read book Protease Inhibitors written by Neha Khandelwal and published by . This book was released on 2013-09 with total page 48 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific Study from the year 2011 in the subject Biology - Human Biology, grade: -, - (National Chemical Laboratory, Pune), course: Post Graduate Doctoral Studies, language: English, abstract: This book chapter covers the previous and current research on plant protease inhibitors being conducted worldwide since its identification. Plant protease inhibitors exists as natural defense mechanism in plants. Solanaceous plants have highly been explored for the protease inhibitor expression and function against various insects of the order lepidoptera, coleoptera, diptera and many more. Plant protease inhibitors are the well studied class of plant defensive proteins. High level of up-regulation upon insect damage, significantly elevated levels in reproductive and storage organs as well as specificity to pest proteases ascertain their defensive function. In the present chapter, we introduced molecular basis of plant-insect interactions and focused on different classes of protease inhibitors based on their target protease(s).
Recombinant Proteins from Plants is one of the most exciting and fastest developing areas in biology. The latest molecular techniques are being applied to the exploitation of plants as novel expression systems for the p- duction and overproduction of heterologous and native proteins. Transgenic plant technology is currently used in three broad areas: the expression of - combinant proteins to improve crop quality by increasing disease/pest res- tance or increasing tolerance to stress, optimizing plant productivity and yield by the genetic manipulation of metabolic pathways, and the large-scale co- effective production of recombinant proteins for use as specialist industrial or therapeutic biomolecules. The intention of Recombinant Proteins from Plants is to provide c- prehensive and detailed protocols covering all the latest molecular approaches. Because the production oftransgenic plants has become routine in many la- ratories, coverage is also given to some of the more "classical" approaches to the separation, analysis, and characterization of recombinant proteins. The book also includes areas of research that we believe will become increasingly important in the near future: efficient transformation of monocots with Agrobacterium optimizing the stability of recombinant proteins, and a section highlighting the immunotherapeutic potential of plant-expressed proteins.
Book Synopsis Recombinant Proteins from Plants by : Charles Cunningham
Download or read book Recombinant Proteins from Plants written by Charles Cunningham and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant Proteins from Plants is one of the most exciting and fastest developing areas in biology. The latest molecular techniques are being applied to the exploitation of plants as novel expression systems for the p- duction and overproduction of heterologous and native proteins. Transgenic plant technology is currently used in three broad areas: the expression of - combinant proteins to improve crop quality by increasing disease/pest res- tance or increasing tolerance to stress, optimizing plant productivity and yield by the genetic manipulation of metabolic pathways, and the large-scale co- effective production of recombinant proteins for use as specialist industrial or therapeutic biomolecules. The intention of Recombinant Proteins from Plants is to provide c- prehensive and detailed protocols covering all the latest molecular approaches. Because the production oftransgenic plants has become routine in many la- ratories, coverage is also given to some of the more "classical" approaches to the separation, analysis, and characterization of recombinant proteins. The book also includes areas of research that we believe will become increasingly important in the near future: efficient transformation of monocots with Agrobacterium optimizing the stability of recombinant proteins, and a section highlighting the immunotherapeutic potential of plant-expressed proteins.
As food producers, plants are constantly under attack by insects. Over the course of evolution, plants have not only developed a sophisticated defense apparatus but have also refined biochemical defense mechanisms to protect themselves, thereby maintaining the ecological balance. Plant-pest interactions induce an elaborate array of reactions involving the release of volatile compounds, effector and signaling molecules, trans-membrane proteins, and a variety of enzymes and hormones. This book offers a comprehensive guide to the strategies that plants employ against insects and other pests to ensure their continued survival. Addressing an important gap in the literature, it shares the latest findings in the field of plant–pest interactions for a broad audience. Providing an overview of the current state of knowledge on plant-pest interactions and their role in the genetic improvement of crops, it offers an essential guide for researchers and professionals in the fields of agriculture, plant pathology, entomology, cell biology, molecular biology and genetics.
Book Synopsis Plant-Pest Interactions: From Molecular Mechanisms to Chemical Ecology by : Indrakant K. Singh
Download or read book Plant-Pest Interactions: From Molecular Mechanisms to Chemical Ecology written by Indrakant K. Singh and published by Springer Nature. This book was released on 2021-01-18 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: As food producers, plants are constantly under attack by insects. Over the course of evolution, plants have not only developed a sophisticated defense apparatus but have also refined biochemical defense mechanisms to protect themselves, thereby maintaining the ecological balance. Plant-pest interactions induce an elaborate array of reactions involving the release of volatile compounds, effector and signaling molecules, trans-membrane proteins, and a variety of enzymes and hormones. This book offers a comprehensive guide to the strategies that plants employ against insects and other pests to ensure their continued survival. Addressing an important gap in the literature, it shares the latest findings in the field of plant–pest interactions for a broad audience. Providing an overview of the current state of knowledge on plant-pest interactions and their role in the genetic improvement of crops, it offers an essential guide for researchers and professionals in the fields of agriculture, plant pathology, entomology, cell biology, molecular biology and genetics.
Book Synopsis Regulation of Proteolysis and Proteome Composition in Plant Response to Environmental Stress by : Mateusz Labudda
Download or read book Regulation of Proteolysis and Proteome Composition in Plant Response to Environmental Stress written by Mateusz Labudda and published by Frontiers Media SA. This book was released on 2022-12-06 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt:
