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Single cell analysis (SCA) is a widely recognized method for elucidating cellular functions that are not accessible through bulk measurements on the population-level. The understanding of genomic sequence at the single cell level can help in developing a better understanding of how various parts of a cell are combined in space and time in order to create dynamic cellular processes. SCA has affected and influenced a variety of scientific fields including immunology, cancer biology, and neuroscience. It involves the miniaturization of conventional engineering concepts in order to fit within the confines of a single cell. The major technologies used for single cell analysis include single-cell sequencing, single-cell imagers, microfluidic droplet technology platforms and flow cytometry. This book explores all the important aspects of single cell analysis in the present day scenario. Those in search of information to further their knowledge will be greatly assisted by it.
Book Synopsis Single Cell Analysis: Technologies and Future Prospects by : Forest Pearson
Download or read book Single Cell Analysis: Technologies and Future Prospects written by Forest Pearson and published by Syrawood Publishing House. This book was released on 2023-09-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single cell analysis (SCA) is a widely recognized method for elucidating cellular functions that are not accessible through bulk measurements on the population-level. The understanding of genomic sequence at the single cell level can help in developing a better understanding of how various parts of a cell are combined in space and time in order to create dynamic cellular processes. SCA has affected and influenced a variety of scientific fields including immunology, cancer biology, and neuroscience. It involves the miniaturization of conventional engineering concepts in order to fit within the confines of a single cell. The major technologies used for single cell analysis include single-cell sequencing, single-cell imagers, microfluidic droplet technology platforms and flow cytometry. This book explores all the important aspects of single cell analysis in the present day scenario. Those in search of information to further their knowledge will be greatly assisted by it.
This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.
Book Synopsis Essentials of Single-Cell Analysis by : Fan-Gang Tseng
Download or read book Essentials of Single-Cell Analysis written by Fan-Gang Tseng and published by Springer. This book was released on 2016-01-21 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.
Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.
Book Synopsis Single Cell Analysis by : Tuhin Subhra Santra
Download or read book Single Cell Analysis written by Tuhin Subhra Santra and published by MDPI. This book was released on 2021-06-02 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.
The resurgence of interest in high-resolution evaluation of single-cell properties has led to examining where current technology stands at the beginning of a new millennium. Engineers and scientists have produced significant advances in cytometric technologies in just the past few years. Emerging Tools for Single-Cell Analysis: Advances in Optical Measurement Technologies stresses the applications and theories behind some of these advances in cell measurement and cell- sorting technologies. Rapid assessment of the proper function of cells and molecular processes within cells is essential. To that end, new and varying technologies present important diagnostic and prognostic tools relevant to a variety of diseases. Future developments in miniaturization of electronics, micro- and nanomachines, and biomedical engineering are certain to impact cell biology. New analytical technologies are revolutionizing our ability to functionally characterize, isolate, and manipulate single cells. This timely book offers researchers and design engineers much-needed information as they further develop technologies for cell analysis. By comparing and contrasting various approaches, the authors explain how those technologies converge toward similar goals: evaluating the properties of cells and sorting cells on those properties using optically-based measurement systems. Emerging Tools for Single-Cell Analysis offers scientists and engineers a vision of the exciting possibilities that exist as new technologies are applied to single-cell analysis,
Book Synopsis Emerging Tools for Single-Cell Analysis by : Gary Durack
Download or read book Emerging Tools for Single-Cell Analysis written by Gary Durack and published by Wiley-Liss. This book was released on 2000-05-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The resurgence of interest in high-resolution evaluation of single-cell properties has led to examining where current technology stands at the beginning of a new millennium. Engineers and scientists have produced significant advances in cytometric technologies in just the past few years. Emerging Tools for Single-Cell Analysis: Advances in Optical Measurement Technologies stresses the applications and theories behind some of these advances in cell measurement and cell- sorting technologies. Rapid assessment of the proper function of cells and molecular processes within cells is essential. To that end, new and varying technologies present important diagnostic and prognostic tools relevant to a variety of diseases. Future developments in miniaturization of electronics, micro- and nanomachines, and biomedical engineering are certain to impact cell biology. New analytical technologies are revolutionizing our ability to functionally characterize, isolate, and manipulate single cells. This timely book offers researchers and design engineers much-needed information as they further develop technologies for cell analysis. By comparing and contrasting various approaches, the authors explain how those technologies converge toward similar goals: evaluating the properties of cells and sorting cells on those properties using optically-based measurement systems. Emerging Tools for Single-Cell Analysis offers scientists and engineers a vision of the exciting possibilities that exist as new technologies are applied to single-cell analysis,
Biosensors for Single-Cell Analysis explores a wide range of biosensor technologies and their applications in single-cell characterization and analysis. Sections cover key biophysical and chemical single-cell properties that consider proteomic, metabolic, electrical, mechanical and optical properties. Each chapter features key definitions and case studies, providing detailed guidance for researchers who want to replicate covered solutions in their work. Tutorial sections, evaluations of the current state-of-the-field and future developments are also included. Microfluidic approaches to characterization, such as microfluidic impedance flow cytometry and microfluidic flow cytometry are considered alongside more conventional approaches, such as mass spectroscopy, fluorescent and mass flow cytometry. Additionally, key types of biosensors are covered, including atomic force microscopy, micropipette aspiration, optical tweezers, microfluidic hydrodynamic stretchers, microfluidic constriction channel and microfluidic optical stretchers. Includes chapters focused on key single-cell properties, such as proteomic, metabolic and mechanical characterization Features case studies that illustrate the application of biosensors for single-cell analysis Considers microfluidic approaches for each single-cell property discussed Explores future directions for single-cell analysis and biosensor technology
Book Synopsis Biosensors for Single-Cell Analysis by : Jian Chen
Download or read book Biosensors for Single-Cell Analysis written by Jian Chen and published by Academic Press. This book was released on 2021-10-26 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biosensors for Single-Cell Analysis explores a wide range of biosensor technologies and their applications in single-cell characterization and analysis. Sections cover key biophysical and chemical single-cell properties that consider proteomic, metabolic, electrical, mechanical and optical properties. Each chapter features key definitions and case studies, providing detailed guidance for researchers who want to replicate covered solutions in their work. Tutorial sections, evaluations of the current state-of-the-field and future developments are also included. Microfluidic approaches to characterization, such as microfluidic impedance flow cytometry and microfluidic flow cytometry are considered alongside more conventional approaches, such as mass spectroscopy, fluorescent and mass flow cytometry. Additionally, key types of biosensors are covered, including atomic force microscopy, micropipette aspiration, optical tweezers, microfluidic hydrodynamic stretchers, microfluidic constriction channel and microfluidic optical stretchers. Includes chapters focused on key single-cell properties, such as proteomic, metabolic and mechanical characterization Features case studies that illustrate the application of biosensors for single-cell analysis Considers microfluidic approaches for each single-cell property discussed Explores future directions for single-cell analysis and biosensor technology
The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology. Based on the author’s personal experience, it provides revealing insights into the potential applications, significance, workflow, comparison, future perspectives and challenges of single-cell sequencing for identifying and developing disease-specific biomarkers in order to understand the biological function, activation and dysfunction of single cells and lymphocytes and to explore their functional roles and responses to therapies. It also provides detailed information on individual subgroups of lymphocytes, including cell characters, function, surface markers, receptor function, intracellular signals and pathways, production of inflammatory mediators, nuclear receptors and factors, omics, sequencing, disease-specific biomarkers, bioinformatics, networks and dynamic networks, their role in disease and future prospects. Dr. Xiangdong Wang is a Professor of Medicine, Director of Shanghai Institute of Clinical Bioinformatics, Director of Fudan University Center for Clinical Bioinformatics, Director of the Biomedical Research Center of Zhongshan Hospital, Deputy Director of Shanghai Respiratory Research Institute, Shanghai, China.
Book Synopsis Single Cell Sequencing and Systems Immunology by : Xiangdong Wang
Download or read book Single Cell Sequencing and Systems Immunology written by Xiangdong Wang and published by Springer. This book was released on 2015-03-27 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology. Based on the author’s personal experience, it provides revealing insights into the potential applications, significance, workflow, comparison, future perspectives and challenges of single-cell sequencing for identifying and developing disease-specific biomarkers in order to understand the biological function, activation and dysfunction of single cells and lymphocytes and to explore their functional roles and responses to therapies. It also provides detailed information on individual subgroups of lymphocytes, including cell characters, function, surface markers, receptor function, intracellular signals and pathways, production of inflammatory mediators, nuclear receptors and factors, omics, sequencing, disease-specific biomarkers, bioinformatics, networks and dynamic networks, their role in disease and future prospects. Dr. Xiangdong Wang is a Professor of Medicine, Director of Shanghai Institute of Clinical Bioinformatics, Director of Fudan University Center for Clinical Bioinformatics, Director of the Biomedical Research Center of Zhongshan Hospital, Deputy Director of Shanghai Respiratory Research Institute, Shanghai, China.
This book presents an overview of the recent technologies in single molecule and single cell sequencing. These sequencing technologies are revolutionizing the way of the genomic studies and the understanding of complex biological systems. The PacBio sequencer has enabled extremely long-read sequencing and the MinION sequencer has made the sequencing possible in developing countries. New developments and technologies are constantly emerging, which will further expand sequencing applications. In parallel, single cell sequencing technologies are rapidly becoming a popular platform. This volume presents not only an updated overview of these technologies, but also of the related developments in bioinformatics. Without powerful bioinformatics software, where rapid progress is taking place, these new technologies will not realize their full potential. All the contributors to this volume have been involved in the development of these technologies and software and have also made significant progress on their applications. This book is intended to be of interest to a wide audience ranging from genome researchers to basic molecular biologists and clinicians.
Book Synopsis Single Molecule and Single Cell Sequencing by : Yutaka Suzuki
Download or read book Single Molecule and Single Cell Sequencing written by Yutaka Suzuki and published by Springer. This book was released on 2019-04-09 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents an overview of the recent technologies in single molecule and single cell sequencing. These sequencing technologies are revolutionizing the way of the genomic studies and the understanding of complex biological systems. The PacBio sequencer has enabled extremely long-read sequencing and the MinION sequencer has made the sequencing possible in developing countries. New developments and technologies are constantly emerging, which will further expand sequencing applications. In parallel, single cell sequencing technologies are rapidly becoming a popular platform. This volume presents not only an updated overview of these technologies, but also of the related developments in bioinformatics. Without powerful bioinformatics software, where rapid progress is taking place, these new technologies will not realize their full potential. All the contributors to this volume have been involved in the development of these technologies and software and have also made significant progress on their applications. This book is intended to be of interest to a wide audience ranging from genome researchers to basic molecular biologists and clinicians.
Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.
Book Synopsis Single Cell Analysis by : Tuhin Subhra Santra
Download or read book Single Cell Analysis written by Tuhin Subhra Santra and published by . This book was released on 2021 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.
This book provides a brief overview of single-cell analysis using recent advanced technologies. The different sections cover different aspect of single cell analysis and applications with their advantages, limitations, and future challenges. The book has covered how different physical energies such as optical, electrical, and mechanical energy have been applied for single cell therapy and analysis. The recent advanced micro/nanofluidic devices have been employed for single-cell counting, manipulation, cultivation, separation, isolation, lysis, printing and patterning and host-viral interaction at single-cell level. Various chemical approaches for single-cell analysis have been discussed, such as liposome mediated materials transfer at single-cell and their analysis, discovery of antibody via single-cell, high-throughput screening of antigen-specific antibody-secreting cells, and biomolecular secretion analysis of individual cells. Moreover, different single-cell omics such as genomics, proteomics and transcriptomics have been discussed using microfluidic technologies as well as conventional approaches. The role of single cell analysis in system biology and biocatalysis have been discussed in detail. The book describes single-cell phenotyping of heterogeneous tissue, stimulation, and instant reaction quenching technology for biochemical kinetic analysis, large scale single-cell assay for the identification of biocatalysts and analytical techniques for single-cell studies in microbiology. The role of single-cell analysis in cancer, such as single-cell adhesion and cancer progression, single-cell technologies for cancer therapy, analytical technology for single cancer cell analysis, and biophysical markers for cancer cell analysis have been discussed. The flow cytometry based high throughput single-cell analysis have been well emphasized. Finally this book has covered single-cell electrophysiology, single-cell sensing and size measurement using mechanical and microwave resonators, molecular force spectroscopy for cell adhesion measurement, micro-tweezers and force microscopy techniques for single-cell mechanobiological analysis, mass spectrometry and acoustic tweezers for single-cell manipulation and analysis. This book is intended for academic and industrial researchers, undergraduate and graduate students in the fields of biomedical engineering, bio-micro/nanoengineering, and bio-micro/nano fabrication for single-cell analysis. It can be used for courses on bio-MEMS/bio-NEMS, biomicrofluidics, bio-micro/nanofabrications, micro/nanofluidics, biophysics, single cell analysis, bionanotechnology, drug delivery systems and biomedical microdevices. Collective contributions from respected experts, have brought diverse aspects of single-cell technologies in a single hand book. This will benefit researchers and practitioners in the biotechnology industry for different diseases analysis, therapeutics, diagnostics, drug discovery, drug screening etc. In addition to hard copies, the book will be available online and will often be updated by the authors.
Book Synopsis Handbook of Single-Cell Technologies by : Tuhin Subhra Santra
Download or read book Handbook of Single-Cell Technologies written by Tuhin Subhra Santra and published by Springer. This book was released on 2021-10-29 with total page 1096 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a brief overview of single-cell analysis using recent advanced technologies. The different sections cover different aspect of single cell analysis and applications with their advantages, limitations, and future challenges. The book has covered how different physical energies such as optical, electrical, and mechanical energy have been applied for single cell therapy and analysis. The recent advanced micro/nanofluidic devices have been employed for single-cell counting, manipulation, cultivation, separation, isolation, lysis, printing and patterning and host-viral interaction at single-cell level. Various chemical approaches for single-cell analysis have been discussed, such as liposome mediated materials transfer at single-cell and their analysis, discovery of antibody via single-cell, high-throughput screening of antigen-specific antibody-secreting cells, and biomolecular secretion analysis of individual cells. Moreover, different single-cell omics such as genomics, proteomics and transcriptomics have been discussed using microfluidic technologies as well as conventional approaches. The role of single cell analysis in system biology and biocatalysis have been discussed in detail. The book describes single-cell phenotyping of heterogeneous tissue, stimulation, and instant reaction quenching technology for biochemical kinetic analysis, large scale single-cell assay for the identification of biocatalysts and analytical techniques for single-cell studies in microbiology. The role of single-cell analysis in cancer, such as single-cell adhesion and cancer progression, single-cell technologies for cancer therapy, analytical technology for single cancer cell analysis, and biophysical markers for cancer cell analysis have been discussed. The flow cytometry based high throughput single-cell analysis have been well emphasized. Finally this book has covered single-cell electrophysiology, single-cell sensing and size measurement using mechanical and microwave resonators, molecular force spectroscopy for cell adhesion measurement, micro-tweezers and force microscopy techniques for single-cell mechanobiological analysis, mass spectrometry and acoustic tweezers for single-cell manipulation and analysis. This book is intended for academic and industrial researchers, undergraduate and graduate students in the fields of biomedical engineering, bio-micro/nanoengineering, and bio-micro/nano fabrication for single-cell analysis. It can be used for courses on bio-MEMS/bio-NEMS, biomicrofluidics, bio-micro/nanofabrications, micro/nanofluidics, biophysics, single cell analysis, bionanotechnology, drug delivery systems and biomedical microdevices. Collective contributions from respected experts, have brought diverse aspects of single-cell technologies in a single hand book. This will benefit researchers and practitioners in the biotechnology industry for different diseases analysis, therapeutics, diagnostics, drug discovery, drug screening etc. In addition to hard copies, the book will be available online and will often be updated by the authors.
The first-ever comprehensive overview of the methods used in this key technology in modern biology provides the latest working knowledge needed by every scientist entering this growing field. It covers all the current technology and application areas, from microscopy and spectroscopy to proteomics and microfluidics.
Book Synopsis Single Cell Analysis by : Dario Anselmetti
Download or read book Single Cell Analysis written by Dario Anselmetti and published by John Wiley & Sons. This book was released on 2009-03-09 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first-ever comprehensive overview of the methods used in this key technology in modern biology provides the latest working knowledge needed by every scientist entering this growing field. It covers all the current technology and application areas, from microscopy and spectroscopy to proteomics and microfluidics.
