Techniques in Insect Immunology

Techniques in Insect Immunology

Author: Andreas Wiesner

Publisher: SOS Publications (NJ)

Published: 1998

Total Pages: 330

ISBN-13:

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The importance of insects for human welfare is unquestionable. This laboratory manual summarizes a number of key methods used by insect immunologists around the world. It should be helpful for those already working in this field & especially for scientists with general interest in this research topic as a methodical guide for the first steps. The experiments are described in detail, with step-by-step directions. Basic procedure for activation, detection, quantification & characterization of cellular & humoral defense reactions are given, along with protocols for more detailed investigations of cell differentiation & isolation of insect immune factors. Population genetic aspect, viral pathology & comparative studies revealing similarities with the vertebrate immune system are also included. The result described in the chapters show remarkable progress in our understanding of insect immunology. Simultaneously, the large number of still-unanswered questions make us realize that we have a lot left to learn about these fascinating animals. From future investigation we can expect a better understanding of the immune system's evolution, the discovery of new antimicrobial & regulatory insect molecules potentially useful in the therapy of human disease & new strategies for insect control.


Book Synopsis Techniques in Insect Immunology by : Andreas Wiesner

Download or read book Techniques in Insect Immunology written by Andreas Wiesner and published by SOS Publications (NJ). This book was released on 1998 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: The importance of insects for human welfare is unquestionable. This laboratory manual summarizes a number of key methods used by insect immunologists around the world. It should be helpful for those already working in this field & especially for scientists with general interest in this research topic as a methodical guide for the first steps. The experiments are described in detail, with step-by-step directions. Basic procedure for activation, detection, quantification & characterization of cellular & humoral defense reactions are given, along with protocols for more detailed investigations of cell differentiation & isolation of insect immune factors. Population genetic aspect, viral pathology & comparative studies revealing similarities with the vertebrate immune system are also included. The result described in the chapters show remarkable progress in our understanding of insect immunology. Simultaneously, the large number of still-unanswered questions make us realize that we have a lot left to learn about these fascinating animals. From future investigation we can expect a better understanding of the immune system's evolution, the discovery of new antimicrobial & regulatory insect molecules potentially useful in the therapy of human disease & new strategies for insect control.

Immunological Techniques in Insect Biology

Immunological Techniques in Insect Biology

Author: Lawrence I. Gilbert

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 291

ISBN-13: 146123798X

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Insects as a group occupy a middle ground in the biosphere between bacteria and viruses at one extreme, amphibians and mammals at the other. The size and general nature of insects present special problems to the study of ento mology. For example, many commercially available instruments are geared to measure in grams, while the forces commonly encountered in studying insects are in the milligram range. Therefore, techniques developed in the study of insects or in those fields concerned with the control of insect pests are often unique. Methods for measuring things are common to all sciences. Advances some times depend more on how something was done than on what was measured; indeed a given field often progresses from one technique to another as new methods are discovered, developed, and modified. Just as often, some of these techniques find their way into the classroom when the problems involved have been sufficiently ironed out to permit students to master the manipulations in a few laboratory periods. Many specialized techniques are confined to one specific research labora tory. Although methods may be considered commonplace where they are used, in another context even the simplest procedures may save considerable time. It is the purpose of this series (1) to report new developments in method ology, (2) to reveal sources of groups who have dealt with and solved particular entomological problems, and (3) to describe experiments which may be appli cable for use in biology laboratory courses.


Book Synopsis Immunological Techniques in Insect Biology by : Lawrence I. Gilbert

Download or read book Immunological Techniques in Insect Biology written by Lawrence I. Gilbert and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: Insects as a group occupy a middle ground in the biosphere between bacteria and viruses at one extreme, amphibians and mammals at the other. The size and general nature of insects present special problems to the study of ento mology. For example, many commercially available instruments are geared to measure in grams, while the forces commonly encountered in studying insects are in the milligram range. Therefore, techniques developed in the study of insects or in those fields concerned with the control of insect pests are often unique. Methods for measuring things are common to all sciences. Advances some times depend more on how something was done than on what was measured; indeed a given field often progresses from one technique to another as new methods are discovered, developed, and modified. Just as often, some of these techniques find their way into the classroom when the problems involved have been sufficiently ironed out to permit students to master the manipulations in a few laboratory periods. Many specialized techniques are confined to one specific research labora tory. Although methods may be considered commonplace where they are used, in another context even the simplest procedures may save considerable time. It is the purpose of this series (1) to report new developments in method ology, (2) to reveal sources of groups who have dealt with and solved particular entomological problems, and (3) to describe experiments which may be appli cable for use in biology laboratory courses.

Immunological Techniques in Insect Biology

Immunological Techniques in Insect Biology

Author: Lawrence I Gilbert

Publisher:

Published: 1988-06-08

Total Pages: 300

ISBN-13: 9781461237990

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Book Synopsis Immunological Techniques in Insect Biology by : Lawrence I Gilbert

Download or read book Immunological Techniques in Insect Biology written by Lawrence I Gilbert and published by . This book was released on 1988-06-08 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Immunity in Insects

Immunity in Insects

Author: Federica Sandrelli

Publisher:

Published: 2020

Total Pages: 246

ISBN-13: 9781071602591

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This volume details methods and protocols necessary to further the study of insect immunity. Chapters guide readers through up-to-date genomic and transcriptomic approaches, insect samples for proteomic analysis, hemocytes in Drosophila, cellular response in Lepidoptera, insect AMPs, manipulate the composition of mosquito microbiota, viral infections in insects, infections by entomopathogenic nematodes, immune response following oral infections, and protocols to to monitor the effect of septic infections with human pathogens using B. mori as a model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunity in Insects aims to ensure successful results in the further study of this vital field.Incl .


Book Synopsis Immunity in Insects by : Federica Sandrelli

Download or read book Immunity in Insects written by Federica Sandrelli and published by . This book was released on 2020 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details methods and protocols necessary to further the study of insect immunity. Chapters guide readers through up-to-date genomic and transcriptomic approaches, insect samples for proteomic analysis, hemocytes in Drosophila, cellular response in Lepidoptera, insect AMPs, manipulate the composition of mosquito microbiota, viral infections in insects, infections by entomopathogenic nematodes, immune response following oral infections, and protocols to to monitor the effect of septic infections with human pathogens using B. mori as a model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunity in Insects aims to ensure successful results in the further study of this vital field.Incl .

Insect Immunology

Insect Immunology

Author: Nancy E. Beckage

Publisher: Academic Press

Published: 2011-04-28

Total Pages: 360

ISBN-13: 9780080556338

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This work is the first book-length publication on the topic of insect immunology since 1991, complementing earlier works by offering a fresh perspective on current research. Interactions of host immune systems with both parasites and pathogens are presented in detail, as well as the genomics and proteomics, approaches which have been lacking in other publications. Beckage provides comprehensive coverage of topics important to medical researchers, including Drosophila as a model for studying cellular and humoral immune mechanisms, biochemical mediators of immunity, and insect blood cells and their functions. Encompasses the most important topics of insect immunology including mechanisms, genes, proteins, evolution and phylogeny Provides comprehensive coverage of topics important to medical researchers including Drosophila as a model for studying cellular and humoral immune mechanisms, biochemical mediators of immunity, and insect blood cells and their functions Most up-to-date information published with contributions from international leaders in the field


Book Synopsis Insect Immunology by : Nancy E. Beckage

Download or read book Insect Immunology written by Nancy E. Beckage and published by Academic Press. This book was released on 2011-04-28 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work is the first book-length publication on the topic of insect immunology since 1991, complementing earlier works by offering a fresh perspective on current research. Interactions of host immune systems with both parasites and pathogens are presented in detail, as well as the genomics and proteomics, approaches which have been lacking in other publications. Beckage provides comprehensive coverage of topics important to medical researchers, including Drosophila as a model for studying cellular and humoral immune mechanisms, biochemical mediators of immunity, and insect blood cells and their functions. Encompasses the most important topics of insect immunology including mechanisms, genes, proteins, evolution and phylogeny Provides comprehensive coverage of topics important to medical researchers including Drosophila as a model for studying cellular and humoral immune mechanisms, biochemical mediators of immunity, and insect blood cells and their functions Most up-to-date information published with contributions from international leaders in the field

The Insect Immune System as a Target for Protecting Beneficial Insects and Controlling Pests

The Insect Immune System as a Target for Protecting Beneficial Insects and Controlling Pests

Author: Arash Zibaee

Publisher: Frontiers Media SA

Published: 2020-12-01

Total Pages: 132

ISBN-13: 2889662268

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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.


Book Synopsis The Insect Immune System as a Target for Protecting Beneficial Insects and Controlling Pests by : Arash Zibaee

Download or read book The Insect Immune System as a Target for Protecting Beneficial Insects and Controlling Pests written by Arash Zibaee and published by Frontiers Media SA. This book was released on 2020-12-01 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Immunology of Insects and Other Arthropods

Immunology of Insects and Other Arthropods

Author: Ayodhya P. Gupta

Publisher: CRC Press

Published: 2019-08-08

Total Pages: 849

ISBN-13: 135109033X

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In insect and other arthropod immune systems, discrimination between self and nonself tissues is accomplished through the combined actions of two immunocytes and several humoral factors. Immunology of Insects and Other Arthropods presents a comprehensive look at this and other important topics in arthropod immunology. Issues discussed include insect immunocytes and other hemocytes, including computer image analysis of immunocyte serial sections; the two basic cellular immune reactions (phagocytosis and encapsulation), including the molecular basis and roles of gap junctions in encapsulation; how encapsulation is affected by polydnavirus and encapsulation-promoting factors; why insect cells are immune to HIV; humoral factors; and antibacterial factors in Lepidoptera, Diptera, and other insect orders. Other topics include hemolymph proteins interacting with mammalian complement cascade; adaptive humoral response in the American cockroach; antigenic stimulation of hemaglutinin production in insects; and the applications of the Limulus Amebocyte Lysate (LAL) in detecting endotoxins in pharmaceuticals, medical devices, clinical diagnosis, and hygienic control. This book represents an important reference source for hematologists, pathologists, immunologists, AIDS researchers, comparative immunologists, and pharmaceutical companies.


Book Synopsis Immunology of Insects and Other Arthropods by : Ayodhya P. Gupta

Download or read book Immunology of Insects and Other Arthropods written by Ayodhya P. Gupta and published by CRC Press. This book was released on 2019-08-08 with total page 849 pages. Available in PDF, EPUB and Kindle. Book excerpt: In insect and other arthropod immune systems, discrimination between self and nonself tissues is accomplished through the combined actions of two immunocytes and several humoral factors. Immunology of Insects and Other Arthropods presents a comprehensive look at this and other important topics in arthropod immunology. Issues discussed include insect immunocytes and other hemocytes, including computer image analysis of immunocyte serial sections; the two basic cellular immune reactions (phagocytosis and encapsulation), including the molecular basis and roles of gap junctions in encapsulation; how encapsulation is affected by polydnavirus and encapsulation-promoting factors; why insect cells are immune to HIV; humoral factors; and antibacterial factors in Lepidoptera, Diptera, and other insect orders. Other topics include hemolymph proteins interacting with mammalian complement cascade; adaptive humoral response in the American cockroach; antigenic stimulation of hemaglutinin production in insects; and the applications of the Limulus Amebocyte Lysate (LAL) in detecting endotoxins in pharmaceuticals, medical devices, clinical diagnosis, and hygienic control. This book represents an important reference source for hematologists, pathologists, immunologists, AIDS researchers, comparative immunologists, and pharmaceutical companies.

Insect Infection and Immunity

Insect Infection and Immunity

Author: Jens Rolff

Publisher: OUP Oxford

Published: 2009-06-25

Total Pages: 272

ISBN-13: 0191579149

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Under continual attack from both microbial pathogens and multicellular parasites, insects must cope with immune challenges every day of their lives. However, this has not prevented them from becoming the most successful group of animals on the planet. Insects possess highly-developed innate immune systems which have been fine-tuned by an arms race with pathogens spanning hundreds of millions of years of evolutionary history. Recent discoveries are revealing both an unexpected degree of specificity and an indication of immunological memory - the functional hallmark of vertebrate immunity. The study of insect immune systems has accelerated rapidly in recent years and is now becoming an important interdisciplinary field. Furthermore, insects are a phenomenally rich and diverse source of antimicrobial chemicals. Some of these are already being seriously considered as potential therapeutic agents to control microbes such as MRSA. Despite a burgeoning interest in the field, this is the first book to provide a coherent synthesis and is clearly structured around two broadly themed sections: mechanisms of immunity and evolutionary ecology. This novel text adopts an interdisciplinary and concept-driven approach, integrating insights from immunology, molecular biology, ecology, evolutionary biology, parasitology, and epidemiology. It features contributions from an international team of leading experts. Insect Infection and Immunity is suitable for both graduate students and researchers interested in insect immunity from either an evolutionary, genetical, physiological or molecular perspective. Due to its interdisciplinary and concept-driven approach, it will also appeal to a broader audience of immunologists, parasitologists and evolutionary biologists requiring a concise overview.


Book Synopsis Insect Infection and Immunity by : Jens Rolff

Download or read book Insect Infection and Immunity written by Jens Rolff and published by OUP Oxford. This book was released on 2009-06-25 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Under continual attack from both microbial pathogens and multicellular parasites, insects must cope with immune challenges every day of their lives. However, this has not prevented them from becoming the most successful group of animals on the planet. Insects possess highly-developed innate immune systems which have been fine-tuned by an arms race with pathogens spanning hundreds of millions of years of evolutionary history. Recent discoveries are revealing both an unexpected degree of specificity and an indication of immunological memory - the functional hallmark of vertebrate immunity. The study of insect immune systems has accelerated rapidly in recent years and is now becoming an important interdisciplinary field. Furthermore, insects are a phenomenally rich and diverse source of antimicrobial chemicals. Some of these are already being seriously considered as potential therapeutic agents to control microbes such as MRSA. Despite a burgeoning interest in the field, this is the first book to provide a coherent synthesis and is clearly structured around two broadly themed sections: mechanisms of immunity and evolutionary ecology. This novel text adopts an interdisciplinary and concept-driven approach, integrating insights from immunology, molecular biology, ecology, evolutionary biology, parasitology, and epidemiology. It features contributions from an international team of leading experts. Insect Infection and Immunity is suitable for both graduate students and researchers interested in insect immunity from either an evolutionary, genetical, physiological or molecular perspective. Due to its interdisciplinary and concept-driven approach, it will also appeal to a broader audience of immunologists, parasitologists and evolutionary biologists requiring a concise overview.

Neurochemical Techniques in Insect Research

Neurochemical Techniques in Insect Research

Author: H. Breer

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 337

ISBN-13: 3642700454

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Neurochemical Techniques in Insect Research properly emphasizes the insect. It only scratches the surface of the exploding repertoire of general neuro chemical techniques that can be applied to insect research in 1985. But it al so presents the advantages of using insects for studying certain biological questions that are approachable by neurochemical techniques. Even more so, it summarizes the long list of unique problems encountered in attempting to study insects by neurochemical techniques. As in other volumes of this series, the contributors to this volume are the authorities in the field. They themselves have developed much of the material presented. Thus the sum effort provides a true description of the state of the art; and, pleasantly, it does so in a very complete and clear manner. Readers of this series will not need to be reminded that, despite the fact that vertebrates make up only about 3% of all animal species, research in in vertebrates such as insects has lagged behind that on vertebrates, at least in the neurochemistry area; the relative simplicity and large cell size of the in sect nervous system has always provided incentive for work in neurophysiol ogy and neuroanatomy. Toxicology interests will always stimulate a certain amount of work on insect neuropharmacology, and insects are extremely suitable for several areas of toxin research. Last but not least, the insects are beautiful organisms for which the applications of genetics can be made to the study of nervous system function.


Book Synopsis Neurochemical Techniques in Insect Research by : H. Breer

Download or read book Neurochemical Techniques in Insect Research written by H. Breer and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: Neurochemical Techniques in Insect Research properly emphasizes the insect. It only scratches the surface of the exploding repertoire of general neuro chemical techniques that can be applied to insect research in 1985. But it al so presents the advantages of using insects for studying certain biological questions that are approachable by neurochemical techniques. Even more so, it summarizes the long list of unique problems encountered in attempting to study insects by neurochemical techniques. As in other volumes of this series, the contributors to this volume are the authorities in the field. They themselves have developed much of the material presented. Thus the sum effort provides a true description of the state of the art; and, pleasantly, it does so in a very complete and clear manner. Readers of this series will not need to be reminded that, despite the fact that vertebrates make up only about 3% of all animal species, research in in vertebrates such as insects has lagged behind that on vertebrates, at least in the neurochemistry area; the relative simplicity and large cell size of the in sect nervous system has always provided incentive for work in neurophysiol ogy and neuroanatomy. Toxicology interests will always stimulate a certain amount of work on insect neuropharmacology, and insects are extremely suitable for several areas of toxin research. Last but not least, the insects are beautiful organisms for which the applications of genetics can be made to the study of nervous system function.

Insect Molecular Biology and Biochemistry

Insect Molecular Biology and Biochemistry

Author: Lawrence I. Gilbert

Publisher: Academic Press

Published: 2011-08-16

Total Pages: 575

ISBN-13: 0123847486

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The publication of the extensive seven-volume work Comprehensive Molecular Insect Science provided a complete reference encompassing important developments and achievements in modern insect science. One of the most swiftly moving areas in entomological and comparative research is molecular biology, and this volume, Insect Molecular Biology and Biochemistry, is designed for those who desire a comprehensive yet concise work on important aspects of this topic. This volume contains ten fully revised or rewritten chapters from the original series as well as five completely new chapters on topics such as insect immunology, insect genomics, RNAi, and molecular biology of circadian rhythms and circadian behavior. The topics included are key to an understanding of insect development, with emphasis on the cuticle, digestive properties, and the transport of lipids; extensive and integrated chapters on cytochrome P450s; and the role of transposable elements in the developmental processes as well as programmed cell death. This volume will be of great value to senior investigators, graduate students, post-doctoral fellows and advanced undergraduate research students. It can also be used as a reference for graduate courses and seminars on the topic. Chapters will also be valuable to the applied biologist or entomologist, providing the requisite understanding necessary for probing the more applied research areas related to insect control. Topics specially selected by the editor-in-chief of the original major reference work Fully revised and new contributions bring together the latest research in the rapidly moving fields of insect molecular biology and insect biochemistry, including coverage of development, physiology, immunity and proteomics Full-color provides readers with clear, useful illustrations to highlight important research findings


Book Synopsis Insect Molecular Biology and Biochemistry by : Lawrence I. Gilbert

Download or read book Insect Molecular Biology and Biochemistry written by Lawrence I. Gilbert and published by Academic Press. This book was released on 2011-08-16 with total page 575 pages. Available in PDF, EPUB and Kindle. Book excerpt: The publication of the extensive seven-volume work Comprehensive Molecular Insect Science provided a complete reference encompassing important developments and achievements in modern insect science. One of the most swiftly moving areas in entomological and comparative research is molecular biology, and this volume, Insect Molecular Biology and Biochemistry, is designed for those who desire a comprehensive yet concise work on important aspects of this topic. This volume contains ten fully revised or rewritten chapters from the original series as well as five completely new chapters on topics such as insect immunology, insect genomics, RNAi, and molecular biology of circadian rhythms and circadian behavior. The topics included are key to an understanding of insect development, with emphasis on the cuticle, digestive properties, and the transport of lipids; extensive and integrated chapters on cytochrome P450s; and the role of transposable elements in the developmental processes as well as programmed cell death. This volume will be of great value to senior investigators, graduate students, post-doctoral fellows and advanced undergraduate research students. It can also be used as a reference for graduate courses and seminars on the topic. Chapters will also be valuable to the applied biologist or entomologist, providing the requisite understanding necessary for probing the more applied research areas related to insect control. Topics specially selected by the editor-in-chief of the original major reference work Fully revised and new contributions bring together the latest research in the rapidly moving fields of insect molecular biology and insect biochemistry, including coverage of development, physiology, immunity and proteomics Full-color provides readers with clear, useful illustrations to highlight important research findings