Testing and Analysis of GMO-containing Foods and Feed

Testing and Analysis of GMO-containing Foods and Feed

Author: Salah E. O. Mahgoub

Publisher: CRC Press

Published: 2019-01-15

Total Pages: 329

ISBN-13: 0429685327

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An increasing number of genetically modified organisms (GMOs) continues to be produced every day. In response to the concerns raised by the development of GMOs and their incorporation in foods and feed, guidelines and regulations to govern and control the use of GMOs and their products have been enacted. These regulations necessitated the design of methods to detect and analyse the presence of GMOs or their products in agriculture produce, food and feed production chains. Design of techniques and instruments that would detect, identify, and quantify GM ingredients in food and feed will help inspection authorities to relay reliable information to consumers who might be concerned about the presence of GM ingredients. Information generated by detection of GMOs in food and feed would be helpful for setting regulations that govern the use of GM components as well as for labeling purposes. Qualitative detection methods of GM-DNA sequences in foods and feeds have evolved fast during the past few years. There is continuous need for the development of more advanced multi-detection systems and for periodic updates of the databases related to these systems. Testing and Analysis of GMO-containing Foods and Feed presents updates and comprehensive views on the various methods and techniques in use today for the detection, identification and quantification of GMOs in foods and feed. The eleven book chapters cover recent developments on sample preparation techniques, immunoassays methods and the PCR technique used in GMO analysis, the use of biosensors in relation to GMO analysis, the application of nucleic acid microarrays for the detection of GMOs, validation and standardization methods for GMO testing, in addition to the type of reference material and reference methods used in GMO testing and analysis. Some of the ISO standards designed for identifying and detecting the presence of GM material in foods are also presented in the book.


Book Synopsis Testing and Analysis of GMO-containing Foods and Feed by : Salah E. O. Mahgoub

Download or read book Testing and Analysis of GMO-containing Foods and Feed written by Salah E. O. Mahgoub and published by CRC Press. This book was released on 2019-01-15 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: An increasing number of genetically modified organisms (GMOs) continues to be produced every day. In response to the concerns raised by the development of GMOs and their incorporation in foods and feed, guidelines and regulations to govern and control the use of GMOs and their products have been enacted. These regulations necessitated the design of methods to detect and analyse the presence of GMOs or their products in agriculture produce, food and feed production chains. Design of techniques and instruments that would detect, identify, and quantify GM ingredients in food and feed will help inspection authorities to relay reliable information to consumers who might be concerned about the presence of GM ingredients. Information generated by detection of GMOs in food and feed would be helpful for setting regulations that govern the use of GM components as well as for labeling purposes. Qualitative detection methods of GM-DNA sequences in foods and feeds have evolved fast during the past few years. There is continuous need for the development of more advanced multi-detection systems and for periodic updates of the databases related to these systems. Testing and Analysis of GMO-containing Foods and Feed presents updates and comprehensive views on the various methods and techniques in use today for the detection, identification and quantification of GMOs in foods and feed. The eleven book chapters cover recent developments on sample preparation techniques, immunoassays methods and the PCR technique used in GMO analysis, the use of biosensors in relation to GMO analysis, the application of nucleic acid microarrays for the detection of GMOs, validation and standardization methods for GMO testing, in addition to the type of reference material and reference methods used in GMO testing and analysis. Some of the ISO standards designed for identifying and detecting the presence of GM material in foods are also presented in the book.

How to Reliably Test for GMOs

How to Reliably Test for GMOs

Author: Jana Žel

Publisher: Springer Science & Business Media

Published: 2011-10-26

Total Pages: 110

ISBN-13: 1461413907

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The detection of genetically modified organisms (GMOs) is becoming very complex, with new GMOs, approved and unapproved, constantly entering world markets. Traceability and labelling of GMOs is defined in regulations worldwide, demanding accurate and reliable testing to support the requirements of legislation. This Brief provides the current state-of-the-art on all key topics involved in GMO testing and is a source of detailed practical information for laboratories. Special focus is given to qualitative and quantitative real-time PCR analysis relevant to all areas where detection and identification rely on nucleic acid-based methods. The following topics, important for testing laboratories, are also discussed: organization of the laboratory, focusing on aspects of the quality system and methods for testing, validation and verification of methods, and measurement uncertainty. The Brief also discusses the new challenges of GMOs and novel modified organisms, using new technologies, and the possible solutions for GMO detection, including bioinformatics tools. Finally, legislation on GMOs and sources of information on GMOs are provided, which are relevant not only to testing laboratories, but to anyone interested in GMOs. The authors of this Brief have many years of experience in GMO testing, development of real-time PCR methods, implementation of quality system requirements, validations and verification of methods, and measurement uncertainty. The National Institute of Biology is a highly qualified research laboratory and a National Reference Laboratory, which also performs routine analyses of food, feed and seed. The Institute for Health and Consumer Protection of the European Union Joint Research Centre has extensive knowledge and experience of GMO detection. It hosts the European Union Reference Laboratory for GM Food and Feed in addition to chairing the European Network of GMO Laboratories.


Book Synopsis How to Reliably Test for GMOs by : Jana Žel

Download or read book How to Reliably Test for GMOs written by Jana Žel and published by Springer Science & Business Media. This book was released on 2011-10-26 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt: The detection of genetically modified organisms (GMOs) is becoming very complex, with new GMOs, approved and unapproved, constantly entering world markets. Traceability and labelling of GMOs is defined in regulations worldwide, demanding accurate and reliable testing to support the requirements of legislation. This Brief provides the current state-of-the-art on all key topics involved in GMO testing and is a source of detailed practical information for laboratories. Special focus is given to qualitative and quantitative real-time PCR analysis relevant to all areas where detection and identification rely on nucleic acid-based methods. The following topics, important for testing laboratories, are also discussed: organization of the laboratory, focusing on aspects of the quality system and methods for testing, validation and verification of methods, and measurement uncertainty. The Brief also discusses the new challenges of GMOs and novel modified organisms, using new technologies, and the possible solutions for GMO detection, including bioinformatics tools. Finally, legislation on GMOs and sources of information on GMOs are provided, which are relevant not only to testing laboratories, but to anyone interested in GMOs. The authors of this Brief have many years of experience in GMO testing, development of real-time PCR methods, implementation of quality system requirements, validations and verification of methods, and measurement uncertainty. The National Institute of Biology is a highly qualified research laboratory and a National Reference Laboratory, which also performs routine analyses of food, feed and seed. The Institute for Health and Consumer Protection of the European Union Joint Research Centre has extensive knowledge and experience of GMO detection. It hosts the European Union Reference Laboratory for GM Food and Feed in addition to chairing the European Network of GMO Laboratories.

Control of GMO Content in Seed and Feed

Control of GMO Content in Seed and Feed

Author: Nordic Council of Ministers

Publisher: Nordic Council of Ministers

Published: 2004

Total Pages: 77

ISBN-13: 9289310340

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Great efforts are made to develop and validate methods of analysis of Genetically Modified Organism (GMO) contents in seed, food and feed in order to be able to comply with the new EU regulations on genetically modified food and feed and on traceability and labeling of genetically modified organisms. This report describes some of the current possibilities and limitations regarding analysis of genetically modified contents in seed and feed. This final part of this report contains an overview of the current GMO analysis situation regarding seed and feed in the individual Nordic countries.


Book Synopsis Control of GMO Content in Seed and Feed by : Nordic Council of Ministers

Download or read book Control of GMO Content in Seed and Feed written by Nordic Council of Ministers and published by Nordic Council of Ministers. This book was released on 2004 with total page 77 pages. Available in PDF, EPUB and Kindle. Book excerpt: Great efforts are made to develop and validate methods of analysis of Genetically Modified Organism (GMO) contents in seed, food and feed in order to be able to comply with the new EU regulations on genetically modified food and feed and on traceability and labeling of genetically modified organisms. This report describes some of the current possibilities and limitations regarding analysis of genetically modified contents in seed and feed. This final part of this report contains an overview of the current GMO analysis situation regarding seed and feed in the individual Nordic countries.

Testing of Genetically Modified Organisms in Foods

Testing of Genetically Modified Organisms in Foods

Author: Farid Ahmed

Publisher: CRC Press

Published: 2004-04-07

Total Pages: 348

ISBN-13: 9781560222743

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Examine several methods of testing for genetically modified organisms and the reasons behind their strict regulation! Testing of Genetically Modified Organisms in Foods is the first study of the screening methods and tools utilized for determining the presence of genetically modified organisms (GMOs) in food products. Leading experts in science, medicine, and government agencies examine the significant research and clinical developments in bio-engineered agriculture to bring you an accurate risk assessment of GMOs in relation to human consumption, economics, and the environment. This book focuses on three high-profile biotechnological commercial aspects of GMO inclusion in the world market: insect resistance, herbicide tolerance, and virus resistance. It also identifies new GM food crops that are in the laboratory and may soon be on your table. Testing of Genetically Modified Organisms in Foods looks at GMOs from the perspectives of both sides of the globe—the European Union and the United States Department of Agriculture—who each have their own set of rules and opinions regarding safety issues and marketing of bioengineered food products. This book looks at the government standards of scientific testing for GMOs, and several chapters specifically analyze current screening methods. This book also explores the impact of GMOs on farming, agricultural economy, pesticide control, and world famine. Testing of Genetically Modified Organisms in Foods brings you current information on: the risks and benefits of agricultural biotechnology—to people and the environment the regulations and protocols of testing for GMOs that have been adopted by European and United States agencies scientific techniques that test for GMOs, including certified reference materials (CRMs) and matrix-based, protein-based, and DNA-based methods of testing the limitations of today’s GMO screening methods and the benefits of alternatives that may be used in the future the long-term risks associated with gene flow of GMOs to other plants, specifically focusing on liabilities, regulatory climates, and intellectual property rights Testing of Genetically Modified Organisms in Foods is generously enhanced with figures, tables, and graphs as well as references at the end of every chapter. The commercialization of agricultural biotechnology makes this text essential for scientists, planners, and students of food, agriculture and environmental science. Government officials and activists will find this book invaluable in debating current issues of agricultural biotechnology and food safety.


Book Synopsis Testing of Genetically Modified Organisms in Foods by : Farid Ahmed

Download or read book Testing of Genetically Modified Organisms in Foods written by Farid Ahmed and published by CRC Press. This book was released on 2004-04-07 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Examine several methods of testing for genetically modified organisms and the reasons behind their strict regulation! Testing of Genetically Modified Organisms in Foods is the first study of the screening methods and tools utilized for determining the presence of genetically modified organisms (GMOs) in food products. Leading experts in science, medicine, and government agencies examine the significant research and clinical developments in bio-engineered agriculture to bring you an accurate risk assessment of GMOs in relation to human consumption, economics, and the environment. This book focuses on three high-profile biotechnological commercial aspects of GMO inclusion in the world market: insect resistance, herbicide tolerance, and virus resistance. It also identifies new GM food crops that are in the laboratory and may soon be on your table. Testing of Genetically Modified Organisms in Foods looks at GMOs from the perspectives of both sides of the globe—the European Union and the United States Department of Agriculture—who each have their own set of rules and opinions regarding safety issues and marketing of bioengineered food products. This book looks at the government standards of scientific testing for GMOs, and several chapters specifically analyze current screening methods. This book also explores the impact of GMOs on farming, agricultural economy, pesticide control, and world famine. Testing of Genetically Modified Organisms in Foods brings you current information on: the risks and benefits of agricultural biotechnology—to people and the environment the regulations and protocols of testing for GMOs that have been adopted by European and United States agencies scientific techniques that test for GMOs, including certified reference materials (CRMs) and matrix-based, protein-based, and DNA-based methods of testing the limitations of today’s GMO screening methods and the benefits of alternatives that may be used in the future the long-term risks associated with gene flow of GMOs to other plants, specifically focusing on liabilities, regulatory climates, and intellectual property rights Testing of Genetically Modified Organisms in Foods is generously enhanced with figures, tables, and graphs as well as references at the end of every chapter. The commercialization of agricultural biotechnology makes this text essential for scientists, planners, and students of food, agriculture and environmental science. Government officials and activists will find this book invaluable in debating current issues of agricultural biotechnology and food safety.

Testing and Analysis of GMO-containing Foods and Feed

Testing and Analysis of GMO-containing Foods and Feed

Author: Salah E. O. Mahgoub

Publisher: CRC Press

Published: 2019-01-15

Total Pages: 288

ISBN-13: 135171385X

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An increasing number of genetically modified organisms (GMOs) continues to be produced every day. In response to the concerns raised by the development of GMOs and their incorporation in foods and feed, guidelines and regulations to govern and control the use of GMOs and their products have been enacted. These regulations necessitated the design of methods to detect and analyse the presence of GMOs or their products in agriculture produce, food and feed production chains. Design of techniques and instruments that would detect, identify, and quantify GM ingredients in food and feed will help inspection authorities to relay reliable information to consumers who might be concerned about the presence of GM ingredients. Information generated by detection of GMOs in food and feed would be helpful for setting regulations that govern the use of GM components as well as for labeling purposes. Qualitative detection methods of GM-DNA sequences in foods and feeds have evolved fast during the past few years. There is continuous need for the development of more advanced multi-detection systems and for periodic updates of the databases related to these systems. Testing and Analysis of GMO-containing Foods and Feed presents updates and comprehensive views on the various methods and techniques in use today for the detection, identification and quantification of GMOs in foods and feed. The eleven book chapters cover recent developments on sample preparation techniques, immunoassays methods and the PCR technique used in GMO analysis, the use of biosensors in relation to GMO analysis, the application of nucleic acid microarrays for the detection of GMOs, validation and standardization methods for GMO testing, in addition to the type of reference material and reference methods used in GMO testing and analysis. Some of the ISO standards designed for identifying and detecting the presence of GM material in foods are also presented in the book.


Book Synopsis Testing and Analysis of GMO-containing Foods and Feed by : Salah E. O. Mahgoub

Download or read book Testing and Analysis of GMO-containing Foods and Feed written by Salah E. O. Mahgoub and published by CRC Press. This book was released on 2019-01-15 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: An increasing number of genetically modified organisms (GMOs) continues to be produced every day. In response to the concerns raised by the development of GMOs and their incorporation in foods and feed, guidelines and regulations to govern and control the use of GMOs and their products have been enacted. These regulations necessitated the design of methods to detect and analyse the presence of GMOs or their products in agriculture produce, food and feed production chains. Design of techniques and instruments that would detect, identify, and quantify GM ingredients in food and feed will help inspection authorities to relay reliable information to consumers who might be concerned about the presence of GM ingredients. Information generated by detection of GMOs in food and feed would be helpful for setting regulations that govern the use of GM components as well as for labeling purposes. Qualitative detection methods of GM-DNA sequences in foods and feeds have evolved fast during the past few years. There is continuous need for the development of more advanced multi-detection systems and for periodic updates of the databases related to these systems. Testing and Analysis of GMO-containing Foods and Feed presents updates and comprehensive views on the various methods and techniques in use today for the detection, identification and quantification of GMOs in foods and feed. The eleven book chapters cover recent developments on sample preparation techniques, immunoassays methods and the PCR technique used in GMO analysis, the use of biosensors in relation to GMO analysis, the application of nucleic acid microarrays for the detection of GMOs, validation and standardization methods for GMO testing, in addition to the type of reference material and reference methods used in GMO testing and analysis. Some of the ISO standards designed for identifying and detecting the presence of GM material in foods are also presented in the book.

Guidelines for Sample Preparation Procedures in GMO Analysis

Guidelines for Sample Preparation Procedures in GMO Analysis

Author: Gilbert Berben

Publisher:

Published: 2014

Total Pages: 36

ISBN-13: 9789279447051

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This document aims at providing guidelines for a correct sample preparation in GMO analysis on food, feed, seed, plants and propagating material samples. It has been developed by the European Network of GMO Laboratories (ENGL), specifically by the ENGL working group "sample preparation procedures" upon a mandate of the ENGL steering committee. The structure of the document is based on that of an existing ISO standard (ISO 6498:2012), dealing with sample preparation for feed; starting from this, the procedures were adapted to the needs of GMO detection and the scope concerning matrices was widen to food, feed and seeds. These guidelines cover the key steps of the sample preparation, i.e. the size of the laboratory sample, the mass reduction techniques as well as techniques for comminution and mixing; considerations about the test portion are included and some performance tests to be applied at different steps of the process are provided. While sample preparation, falling after sampling and before analytical testing for GMOs, has suffered from a lack of guidance and harmonization, this document provides detailed and practical hints on this important step, thus contributing to foster testing harmonisation.


Book Synopsis Guidelines for Sample Preparation Procedures in GMO Analysis by : Gilbert Berben

Download or read book Guidelines for Sample Preparation Procedures in GMO Analysis written by Gilbert Berben and published by . This book was released on 2014 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt: This document aims at providing guidelines for a correct sample preparation in GMO analysis on food, feed, seed, plants and propagating material samples. It has been developed by the European Network of GMO Laboratories (ENGL), specifically by the ENGL working group "sample preparation procedures" upon a mandate of the ENGL steering committee. The structure of the document is based on that of an existing ISO standard (ISO 6498:2012), dealing with sample preparation for feed; starting from this, the procedures were adapted to the needs of GMO detection and the scope concerning matrices was widen to food, feed and seeds. These guidelines cover the key steps of the sample preparation, i.e. the size of the laboratory sample, the mass reduction techniques as well as techniques for comminution and mixing; considerations about the test portion are included and some performance tests to be applied at different steps of the process are provided. While sample preparation, falling after sampling and before analytical testing for GMOs, has suffered from a lack of guidance and harmonization, this document provides detailed and practical hints on this important step, thus contributing to foster testing harmonisation.

Safety of Genetically Engineered Foods

Safety of Genetically Engineered Foods

Author: National Research Council

Publisher: National Academies Press

Published: 2004-07-08

Total Pages: 254

ISBN-13: 0309166152

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Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.


Book Synopsis Safety of Genetically Engineered Foods by : National Research Council

Download or read book Safety of Genetically Engineered Foods written by National Research Council and published by National Academies Press. This book was released on 2004-07-08 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Genetically Engineered Crops

Genetically Engineered Crops

Author: National Academies of Sciences, Engineering, and Medicine

Publisher: National Academies Press

Published: 2017-01-28

Total Pages: 607

ISBN-13: 0309437385

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Genetically engineered (GE) crops were first introduced commercially in the 1990s. After two decades of production, some groups and individuals remain critical of the technology based on their concerns about possible adverse effects on human health, the environment, and ethical considerations. At the same time, others are concerned that the technology is not reaching its potential to improve human health and the environment because of stringent regulations and reduced public funding to develop products offering more benefits to society. While the debate about these and other questions related to the genetic engineering techniques of the first 20 years goes on, emerging genetic-engineering technologies are adding new complexities to the conversation. Genetically Engineered Crops builds on previous related Academies reports published between 1987 and 2010 by undertaking a retrospective examination of the purported positive and adverse effects of GE crops and to anticipate what emerging genetic-engineering technologies hold for the future. This report indicates where there are uncertainties about the economic, agronomic, health, safety, or other impacts of GE crops and food, and makes recommendations to fill gaps in safety assessments, increase regulatory clarity, and improve innovations in and access to GE technology.


Book Synopsis Genetically Engineered Crops by : National Academies of Sciences, Engineering, and Medicine

Download or read book Genetically Engineered Crops written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2017-01-28 with total page 607 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetically engineered (GE) crops were first introduced commercially in the 1990s. After two decades of production, some groups and individuals remain critical of the technology based on their concerns about possible adverse effects on human health, the environment, and ethical considerations. At the same time, others are concerned that the technology is not reaching its potential to improve human health and the environment because of stringent regulations and reduced public funding to develop products offering more benefits to society. While the debate about these and other questions related to the genetic engineering techniques of the first 20 years goes on, emerging genetic-engineering technologies are adding new complexities to the conversation. Genetically Engineered Crops builds on previous related Academies reports published between 1987 and 2010 by undertaking a retrospective examination of the purported positive and adverse effects of GE crops and to anticipate what emerging genetic-engineering technologies hold for the future. This report indicates where there are uncertainties about the economic, agronomic, health, safety, or other impacts of GE crops and food, and makes recommendations to fill gaps in safety assessments, increase regulatory clarity, and improve innovations in and access to GE technology.

Guidelines for Sample Preparation Procedures in GMO Analysis

Guidelines for Sample Preparation Procedures in GMO Analysis

Author: Gilbert Berben

Publisher:

Published: 2014

Total Pages: 36

ISBN-13: 9789279447044

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This document aims at providing guidelines for a correct sample preparation in GMO analysis on food, feed, seed, plants and propagating material samples. It has been developed by the European Network of GMO Laboratories (ENGL), specifically by the ENGL working group "sample preparation procedures" upon a mandate of the ENGL steering committee. The structure of the document is based on that of an existing ISO standard (ISO 6498:2012), dealing with sample preparation for feed; starting from this, the procedures were adapted to the needs of GMO detection and the scope concerning matrices was widen to food, feed and seeds. These guidelines cover the key steps of the sample preparation, i.e. the size of the laboratory sample, the mass reduction techniques as well as techniques for comminution and mixing; considerations about the test portion are included and some performance tests to be applied at different steps of the process are provided. While sample preparation, falling after sampling and before analytical testing for GMOs, has suffered from a lack of guidance and harmonization, this document provides detailed and practical hints on this important step, thus contributing to foster testing harmonisation.


Book Synopsis Guidelines for Sample Preparation Procedures in GMO Analysis by : Gilbert Berben

Download or read book Guidelines for Sample Preparation Procedures in GMO Analysis written by Gilbert Berben and published by . This book was released on 2014 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt: This document aims at providing guidelines for a correct sample preparation in GMO analysis on food, feed, seed, plants and propagating material samples. It has been developed by the European Network of GMO Laboratories (ENGL), specifically by the ENGL working group "sample preparation procedures" upon a mandate of the ENGL steering committee. The structure of the document is based on that of an existing ISO standard (ISO 6498:2012), dealing with sample preparation for feed; starting from this, the procedures were adapted to the needs of GMO detection and the scope concerning matrices was widen to food, feed and seeds. These guidelines cover the key steps of the sample preparation, i.e. the size of the laboratory sample, the mass reduction techniques as well as techniques for comminution and mixing; considerations about the test portion are included and some performance tests to be applied at different steps of the process are provided. While sample preparation, falling after sampling and before analytical testing for GMOs, has suffered from a lack of guidance and harmonization, this document provides detailed and practical hints on this important step, thus contributing to foster testing harmonisation.

Definition of Minimum Performance Requirements for Analytical Methods of GMO Testing

Definition of Minimum Performance Requirements for Analytical Methods of GMO Testing

Author:

Publisher:

Published: 2023

Total Pages: 0

ISBN-13: 9789268017456

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The document "Definition of Minimum Performance Requirements for analytical methods of GMO testing" (ENGL 2015) is a guidance developed by the ENGL and the EU Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF). It supports the development and validation of methods for GMO analysis submitted in the frame of applications for EU market authorisation of GMO products, as well as those to be used in the official control of food and feed. The guidance document provides definitions and requirements to assess the performance of the methods developed for the detection and quantification of GMOs based on real-time PCR. More recently, technologies like digital PCR were successfully applied in various fields for the quantification of nucleic acid molecules, including GMOs. Moreover, GM animals and products developed by means of so-called new genomic techniques (Broothaerts et al. 2021) are being released on the market and present additional challenges from the analytical viewpoint. Therefore, ENGL experts have extended the original method performance parameters for guiding users in the development and validation of digital PCR methods for GMO analysis. While the developments in the fields of GM animals and new genomic techniques are still evolving, in addition to the Minimum Performance Requirements guidelines also specific recommendations for methods for the detection and quantification of organisms with short genomic alterations and of GM animals are provided. Given that developments in the fields of GM animals and new genomic techniques are ongoing, these recommendations may be further elaborated in the future.


Book Synopsis Definition of Minimum Performance Requirements for Analytical Methods of GMO Testing by :

Download or read book Definition of Minimum Performance Requirements for Analytical Methods of GMO Testing written by and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The document "Definition of Minimum Performance Requirements for analytical methods of GMO testing" (ENGL 2015) is a guidance developed by the ENGL and the EU Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF). It supports the development and validation of methods for GMO analysis submitted in the frame of applications for EU market authorisation of GMO products, as well as those to be used in the official control of food and feed. The guidance document provides definitions and requirements to assess the performance of the methods developed for the detection and quantification of GMOs based on real-time PCR. More recently, technologies like digital PCR were successfully applied in various fields for the quantification of nucleic acid molecules, including GMOs. Moreover, GM animals and products developed by means of so-called new genomic techniques (Broothaerts et al. 2021) are being released on the market and present additional challenges from the analytical viewpoint. Therefore, ENGL experts have extended the original method performance parameters for guiding users in the development and validation of digital PCR methods for GMO analysis. While the developments in the fields of GM animals and new genomic techniques are still evolving, in addition to the Minimum Performance Requirements guidelines also specific recommendations for methods for the detection and quantification of organisms with short genomic alterations and of GM animals are provided. Given that developments in the fields of GM animals and new genomic techniques are ongoing, these recommendations may be further elaborated in the future.