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Tissue Engineering Methods and Protocols

Author: Jeffrey R. Morgan

Publisher: Springer Science & Business Media

Published: 1998-09-28

Total Pages: 1215

ISBN-13: 0896035166

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In recent years, the field of tissue engineering has begun, in part, to c- lesce around the important clinical goal of developing substitutes or repla- ments for defective tissues or organs. These efforts are focused on many tissues including skin, cartilage, liver, pancreas, bone, blood, muscle, the vascu- ture, and nerves. There is a staggering medical need for new and effective treatments for acquired as well as inherited defects of organs/tissues. Tissue engineering is at the interface of the life sciences, engineering, and clinical medicine and so draws upon advances in cell and molecular biology, mate- als sciences, and surgery, as well as chemical and mechanical engineering. Such an interdisciplinary field requires a broad knowledge base as well as the use of a wide assortment of methods and approaches. It is hoped that by bringing together these protocols, this book will help to form connections - tween the different disciplines and further stimulate the synergism underlying the foundation of the tissue engineering field.

Book Synopsis Tissue Engineering Methods and Protocols by : Jeffrey R. Morgan

Download or read book Tissue Engineering Methods and Protocols written by Jeffrey R. Morgan and published by Springer Science & Business Media. This book was released on 1998-09-28 with total page 1215 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the field of tissue engineering has begun, in part, to c- lesce around the important clinical goal of developing substitutes or repla- ments for defective tissues or organs. These efforts are focused on many tissues including skin, cartilage, liver, pancreas, bone, blood, muscle, the vascu- ture, and nerves. There is a staggering medical need for new and effective treatments for acquired as well as inherited defects of organs/tissues. Tissue engineering is at the interface of the life sciences, engineering, and clinical medicine and so draws upon advances in cell and molecular biology, mate- als sciences, and surgery, as well as chemical and mechanical engineering. Such an interdisciplinary field requires a broad knowledge base as well as the use of a wide assortment of methods and approaches. It is hoped that by bringing together these protocols, this book will help to form connections - tween the different disciplines and further stimulate the synergism underlying the foundation of the tissue engineering field.

Biomaterials for Tissue Engineering

Author: Kanika Chawla

Publisher: Humana

Published: 2019-05-05

Total Pages: 212

ISBN-13: 9781493992713

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This volume provides protocols for the generation of various biomaterials for tissue engineering and regenerative medicine applications. The chapters in this book include a look at a range of biomaterials including hydrogels and other matrices (natural, synthetic, self-healing) for various applications including drug and gene delivery, surface modification and functionalization of biomaterials. In addition, techniques described include those for controlling biomaterial geometry, such as three-dimensional printing and electrospinning. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Biomaterials for Tissue Engineering: Methods and Protocols is a valuable resource for scientists and engineers interested in this vital field of study.

Book Synopsis Biomaterials for Tissue Engineering by : Kanika Chawla

Download or read book Biomaterials for Tissue Engineering written by Kanika Chawla and published by Humana. This book was released on 2019-05-05 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides protocols for the generation of various biomaterials for tissue engineering and regenerative medicine applications. The chapters in this book include a look at a range of biomaterials including hydrogels and other matrices (natural, synthetic, self-healing) for various applications including drug and gene delivery, surface modification and functionalization of biomaterials. In addition, techniques described include those for controlling biomaterial geometry, such as three-dimensional printing and electrospinning. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Biomaterials for Tissue Engineering: Methods and Protocols is a valuable resource for scientists and engineers interested in this vital field of study.

Computer-Aided Tissue Engineering

Author: Alberto Rainer

Publisher: Humana

Published: 2020-07-19

Total Pages: 178

ISBN-13: 9781071606100

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This volume details protocols encompassing different aspects of computer aided design and manufacturing of 3D scaffolds and biofabricated constructs for tissue engineering applications. Chapters are divided into four parts covering optimization of scaffold architectures for computer aided tissue engineering, synthetic routes to biomaterials compatible, technological platforms and manufacturing processes, and relevant applicative scenarios. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computer-Aided Tissue Engineering: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.

Book Synopsis Computer-Aided Tissue Engineering by : Alberto Rainer

Download or read book Computer-Aided Tissue Engineering written by Alberto Rainer and published by Humana. This book was released on 2020-07-19 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details protocols encompassing different aspects of computer aided design and manufacturing of 3D scaffolds and biofabricated constructs for tissue engineering applications. Chapters are divided into four parts covering optimization of scaffold architectures for computer aided tissue engineering, synthetic routes to biomaterials compatible, technological platforms and manufacturing processes, and relevant applicative scenarios. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computer-Aided Tissue Engineering: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.

Vascular Tissue Engineering

Author: Feng Zhao

Publisher: Humana

Published: 2022-10-02

Total Pages: 0

ISBN-13: 9781071617106

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This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using various biomaterials and stem cells, stem cell-derived, or primary vascular cells; biomaterial modification by anticoagulation molecules; vascular bioengineering technologies such as 3D bioprinting; and fabrication of TEVGs with different geometry and multiphase structures. This book also features protocols for grafting and evaluation of vascular grafts in animal models, vascular imaging in animals, and the quantification of blood vessel permeability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Vascular Tissue Engineering: Methods and Protocols is a valuable resource for biomedical engineers, cell biologists, vascular surgeons, doctors, and nurses.

Book Synopsis Vascular Tissue Engineering by : Feng Zhao

Download or read book Vascular Tissue Engineering written by Feng Zhao and published by Humana. This book was released on 2022-10-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using various biomaterials and stem cells, stem cell-derived, or primary vascular cells; biomaterial modification by anticoagulation molecules; vascular bioengineering technologies such as 3D bioprinting; and fabrication of TEVGs with different geometry and multiphase structures. This book also features protocols for grafting and evaluation of vascular grafts in animal models, vascular imaging in animals, and the quantification of blood vessel permeability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Vascular Tissue Engineering: Methods and Protocols is a valuable resource for biomedical engineers, cell biologists, vascular surgeons, doctors, and nurses.

Skin Tissue Engineering

Author: Sophie Böttcher-Haberzeth

Publisher: Springer Nature

Published: 2019-05-30

Total Pages: 260

ISBN-13: 1493994735

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This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine.

Book Synopsis Skin Tissue Engineering by : Sophie Böttcher-Haberzeth

Download or read book Skin Tissue Engineering written by Sophie Böttcher-Haberzeth and published by Springer Nature. This book was released on 2019-05-30 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine.

Cardiac Tissue Engineering

Author: Milica Radisic

Publisher: Humana

Published: 2016-09-24

Total Pages: 0

ISBN-13: 9781493946136

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Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.

Book Synopsis Cardiac Tissue Engineering by : Milica Radisic

Download or read book Cardiac Tissue Engineering written by Milica Radisic and published by Humana. This book was released on 2016-09-24 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.

Methods of Tissue Engineering

Author: Anthony Atala

Publisher: Gulf Professional Publishing

Published: 2001-10-12

Total Pages: 580

ISBN-13: 0124366368

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This reference book combines the tools, experimental protocols, detailed descriptions and know-how for the successful engineering of tissues and organs in one volume.

Book Synopsis Methods of Tissue Engineering by : Anthony Atala

Download or read book Methods of Tissue Engineering written by Anthony Atala and published by Gulf Professional Publishing. This book was released on 2001-10-12 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference book combines the tools, experimental protocols, detailed descriptions and know-how for the successful engineering of tissues and organs in one volume.

Tissue Engineering

Author: Narine Sarvazyan

Publisher: Springer Nature

Published: 2020-04-02

Total Pages: 154

ISBN-13: 3030396983

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Tissue engineering and regenerative medicine is a new, interdisciplinary branch of science that combines knowledge from numerous scientific fields including biology, biochemistry, physics, chemistry, applied engineering, and medicine. It aims to restore damaged parts of the human body by rebuilding them in vitro using individual building blocks of biological tissues such as cells and the extracellular matrix that surrounds them. The authors hope to spark students’ interest in this exciting new field of science as well as give them a basic knowledge of its terminology. This book is based on a hands-on practical course in tissue engineering conducted by the Fulbright US Scholar recipient, Dr. Narine Sarvazyan (George Washington University, Washington USA). It provides an overview of the core topics of the tissue engineering field, including stem cell differentiation, the role of extracellular matrix and attachment proteins, scaffolds, and culturing of engineered tissues. Each chapter is accompanied by hands-on demonstrations and self-check questions. The text is easily readable for students of all backgrounds and the described protocols can be conducted using common lab equipment. This textbook is also useful for developing undergraduate and graduate courses that teach basic methods and approaches in this promising and rapidly developing field.

Book Synopsis Tissue Engineering by : Narine Sarvazyan

Download or read book Tissue Engineering written by Narine Sarvazyan and published by Springer Nature. This book was released on 2020-04-02 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue engineering and regenerative medicine is a new, interdisciplinary branch of science that combines knowledge from numerous scientific fields including biology, biochemistry, physics, chemistry, applied engineering, and medicine. It aims to restore damaged parts of the human body by rebuilding them in vitro using individual building blocks of biological tissues such as cells and the extracellular matrix that surrounds them. The authors hope to spark students’ interest in this exciting new field of science as well as give them a basic knowledge of its terminology. This book is based on a hands-on practical course in tissue engineering conducted by the Fulbright US Scholar recipient, Dr. Narine Sarvazyan (George Washington University, Washington USA). It provides an overview of the core topics of the tissue engineering field, including stem cell differentiation, the role of extracellular matrix and attachment proteins, scaffolds, and culturing of engineered tissues. Each chapter is accompanied by hands-on demonstrations and self-check questions. The text is easily readable for students of all backgrounds and the described protocols can be conducted using common lab equipment. This textbook is also useful for developing undergraduate and graduate courses that teach basic methods and approaches in this promising and rapidly developing field.

Standardisation in Cell and Tissue Engineering

Author: V Salih

Publisher: Elsevier

Published: 2013-07-31

Total Pages: 282

ISBN-13: 0857098721

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The increased use of biodegradable synthetic or natural scaffolds combined with cells and/or biological molecules, in order to create functional replacement tissue in a damaged tissue site, has led to the need for the development of ‘best practice’ methods in the area of tissue engineering to help ensure the creation of safe, high quality products. Standardisation in cell and tissue engineering introduces concepts and current practice in the field of cell and tissue engineering to a wide audience and aims to provide awareness of the importance of standardisation in this area while suggesting directions for further investigation. Part one provides an overview of methods for cell and tissue engineering and includes chapters on the fundamentals of cell and matrix biology for tissue engineering, 3D collagen biomatrix development, and control and vascularisation of tissue-engineered constructs. Part two begins with a chapter exploring the methods and protocols of standardisation in cell and tissue engineering before moving on to highlight issues of quality control in cell and tissue engineering, standardised chemical analysis and testing of biomaterials and principles of good laboratory practice (GLP) for in vitro cell culture applications. Standardisation in cell and tissue engineering is a standard reference for leading research groups, government agencies, regulatory bodies, and researchers and technicians at all levels across the whole range of disciplines using cell culture within the pharmaceutical, biotechnology and biomedical industries. Introduces concepts and current practice in the field of cell and tissue engineering Highlights the importance of standardisation in cell and tissue engineering and suggests directions for further investigation Explores methods and protocols of standardisation in cell and tissue engineering and issues of quality control in cell and tissue engineering

Book Synopsis Standardisation in Cell and Tissue Engineering by : V Salih

Download or read book Standardisation in Cell and Tissue Engineering written by V Salih and published by Elsevier. This book was released on 2013-07-31 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increased use of biodegradable synthetic or natural scaffolds combined with cells and/or biological molecules, in order to create functional replacement tissue in a damaged tissue site, has led to the need for the development of ‘best practice’ methods in the area of tissue engineering to help ensure the creation of safe, high quality products. Standardisation in cell and tissue engineering introduces concepts and current practice in the field of cell and tissue engineering to a wide audience and aims to provide awareness of the importance of standardisation in this area while suggesting directions for further investigation. Part one provides an overview of methods for cell and tissue engineering and includes chapters on the fundamentals of cell and matrix biology for tissue engineering, 3D collagen biomatrix development, and control and vascularisation of tissue-engineered constructs. Part two begins with a chapter exploring the methods and protocols of standardisation in cell and tissue engineering before moving on to highlight issues of quality control in cell and tissue engineering, standardised chemical analysis and testing of biomaterials and principles of good laboratory practice (GLP) for in vitro cell culture applications. Standardisation in cell and tissue engineering is a standard reference for leading research groups, government agencies, regulatory bodies, and researchers and technicians at all levels across the whole range of disciplines using cell culture within the pharmaceutical, biotechnology and biomedical industries. Introduces concepts and current practice in the field of cell and tissue engineering Highlights the importance of standardisation in cell and tissue engineering and suggests directions for further investigation Explores methods and protocols of standardisation in cell and tissue engineering and issues of quality control in cell and tissue engineering

Wound Regeneration and Repair

Author: Robert G. Gourdie

Publisher: Humana Press

Published: 2013-09-13

Total Pages: 0

ISBN-13: 9781627035040

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In Wound Regeneration and Repair: Methods and Protocols, expert researchers in the field detail classical and cutting-edge methods for studying wound healing and regeneration. These techniques include cellular and molecular methods, genetic approaches, surgical procedures, clinical advances, drug discovery and delivery modalities, animal and humanized models and new applications in the treatment of pathological wounds in a variety of organs and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, a detailed list of the necessary materials and reagents for each procedure, step-by-step, reproducible laboratory protocols, and a set notes, developed by the authors, for troubleshooting and avoiding known pitfalls. Authoritative and practical, Wound Regeneration and Repair: Methods and Protocols seeks to aid scientists and entrepreneurs in their further study of technologies, models, techniques, and critical new areas and approaches to clinical and commercial translation of research.

Book Synopsis Wound Regeneration and Repair by : Robert G. Gourdie

Download or read book Wound Regeneration and Repair written by Robert G. Gourdie and published by Humana Press. This book was released on 2013-09-13 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Wound Regeneration and Repair: Methods and Protocols, expert researchers in the field detail classical and cutting-edge methods for studying wound healing and regeneration. These techniques include cellular and molecular methods, genetic approaches, surgical procedures, clinical advances, drug discovery and delivery modalities, animal and humanized models and new applications in the treatment of pathological wounds in a variety of organs and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, a detailed list of the necessary materials and reagents for each procedure, step-by-step, reproducible laboratory protocols, and a set notes, developed by the authors, for troubleshooting and avoiding known pitfalls. Authoritative and practical, Wound Regeneration and Repair: Methods and Protocols seeks to aid scientists and entrepreneurs in their further study of technologies, models, techniques, and critical new areas and approaches to clinical and commercial translation of research.